##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632537.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50180
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9005380629733	34.0	31.0	34.0	31.0	34.0
2	33.02979274611399	34.0	33.0	34.0	31.0	34.0
3	33.096950976484656	34.0	33.0	34.0	31.0	34.0
4	36.443981666002394	37.0	37.0	37.0	35.0	37.0
5	36.403925866879234	37.0	37.0	37.0	35.0	37.0
6	36.44834595456357	37.0	37.0	37.0	35.0	37.0
7	36.45464328417696	37.0	37.0	37.0	35.0	37.0
8	36.427879633320046	37.0	37.0	37.0	35.0	37.0
9	38.20721402949382	39.0	39.0	39.0	37.0	39.0
10	38.220785173375845	39.0	39.0	39.0	37.0	39.0
11	38.290115583897965	39.0	39.0	39.0	37.0	39.0
12	38.23322040653647	39.0	39.0	39.0	37.0	39.0
13	38.282243921881225	39.0	39.0	39.0	37.0	39.0
14	39.72887604623356	41.0	40.0	41.0	38.0	41.0
15	39.729174970107614	41.0	40.0	41.0	38.0	41.0
16	39.6800518134715	41.0	40.0	41.0	38.0	41.0
17	39.708290155440416	41.0	40.0	41.0	38.0	41.0
18	39.71319250697489	41.0	40.0	41.0	38.0	41.0
19	39.71187724192905	41.0	40.0	41.0	38.0	41.0
20	39.6919689119171	41.0	40.0	41.0	38.0	41.0
21	39.646153846153844	41.0	40.0	41.0	37.0	41.0
22	39.61540454364289	41.0	40.0	41.0	37.0	41.0
23	39.568832204065366	41.0	40.0	41.0	37.0	41.0
24	39.52600637704265	41.0	39.0	41.0	37.0	41.0
25	39.49097249900359	41.0	39.0	41.0	37.0	41.0
26	39.388282184137104	41.0	39.0	41.0	37.0	41.0
27	39.28068951773615	40.0	39.0	41.0	36.0	41.0
28	39.227401355121565	40.0	39.0	41.0	36.0	41.0
29	39.180011956954964	40.0	39.0	41.0	36.0	41.0
30	39.13485452371463	40.0	39.0	41.0	35.0	41.0
31	39.07748106815464	40.0	39.0	41.0	35.0	41.0
32	39.02178158628936	40.0	39.0	41.0	35.0	41.0
33	38.93501394978079	40.0	39.0	41.0	35.0	41.0
34	38.90619768832204	40.0	39.0	41.0	35.0	41.0
35	38.827799920286964	40.0	38.0	41.0	35.0	41.0
36	38.766121960940616	40.0	38.0	41.0	35.0	41.0
37	38.71899163013153	40.0	38.0	41.0	35.0	41.0
38	38.64717018732563	40.0	38.0	41.0	35.0	41.0
39	38.525946592267836	40.0	38.0	41.0	35.0	41.0
40	38.44340374651255	40.0	38.0	41.0	35.0	41.0
41	38.40645675567956	40.0	38.0	41.0	35.0	41.0
42	38.318573136707855	40.0	38.0	41.0	34.0	41.0
43	37.544619370267036	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	1.0
21	4.0
22	10.0
23	6.0
24	37.0
25	54.0
26	75.0
27	105.0
28	136.0
29	207.0
30	275.0
31	363.0
32	485.0
33	628.0
34	943.0
35	1442.0
36	2386.0
37	4538.0
38	12445.0
39	26036.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.35552012754085	16.233559186927064	13.371861299322438	28.039059386209647
2	22.044639298525308	19.65524113192507	31.319250697489036	26.980868872060583
3	21.566361100039856	19.129135113591072	29.37425269031487	29.930251096054207
4	15.46233559186927	16.841371064168992	34.69111199681148	33.005181347150256
5	17.636508569151058	31.91311279394181	32.774013551215624	17.67636508569151
6	32.02271821442806	34.24671183738541	16.14188919888402	17.58868074930251
7	28.925866879234757	27.630530091669986	19.760860900757272	23.68274212833798
8	26.42487046632124	30.06576325229175	21.582303706656038	21.927062574730968
9	28.571143882024714	12.176165803108809	17.45715424471901	41.79553607014747
10	20.213232363491432	22.56476683937824	27.70625747309685	29.515743324033476
11	39.95815065763252	18.963730569948186	17.90952570745317	23.16859306496612
12	21.91311279394181	23.95177361498605	26.652052610601835	27.48306098047031
13	36.831406935033876	17.128337983260263	19.818652849740932	26.221602231964923
14	24.11917098445596	21.006377042646474	23.134715025906736	31.73973694699083
15	31.050219210840975	23.31805500199283	19.59744918294141	26.034276604224793
16	24.420087684336387	24.350338780390594	22.56476683937824	28.66480669589478
17	27.20605819051415	24.02750099641291	20.66560382622559	28.100836986847348
18	27.504982064567557	19.89637305699482	22.698286169788762	29.900358708648866
19	28.56516540454364	21.881227580709446	22.756078118772418	26.797528895974494
20	31.365085691510565	19.67915504184934	21.93503387803906	27.020725388601036
21	27.72020725388601	21.104025508170583	21.640095655639698	29.535671582303706
22	29.274611398963728	22.9912315663611	20.077720207253886	27.65643682742128
23	29.426066161817456	20.759266640095657	21.675966520526107	28.13870067756078
24	28.324033479473893	20.44041450777202	22.82981267437226	28.405739338381824
25	28.905938620964527	21.550418493423678	22.774013551215624	26.76962933439617
26	29.011558389796733	22.423276205659626	21.594260661618176	26.970904742925466
27	27.048624950179356	21.09206855320845	25.02989238740534	26.829414109206855
28	26.125946592267834	22.91151853328019	21.747708250298924	29.21482662415305
29	27.522917497010763	23.824232762056596	21.504583499402152	27.14826624153049
30	26.835392586687924	21.68593064966122	23.860103626943005	27.61857313670785
31	28.96771622160223	21.351135910721403	22.33160621761658	27.349541650059784
32	25.79912315663611	21.606217616580313	22.319649262654444	30.275009964129136
33	25.99043443603029	20.8330011956955	24.98605021921084	28.19051414906337
34	26.385013949780788	21.167795934635315	24.595456357114386	27.85173375846951
35	25.51016341171782	23.29214826624153	25.32084495815066	25.876843363889996
36	26.460741331207654	21.14986050219211	25.448385811080108	26.941012355520126
37	26.388999601434836	21.28537265842965	24.635312873654843	27.69031486648067
38	25.50617776006377	20.077720207253886	26.504583499402152	27.911518533280194
39	25.013949780789158	18.597050617776006	27.776006377042645	28.612993224392184
40	24.27461139896373	20.488242327620565	29.31446791550418	25.922678357911515
41	22.497010761259467	19.057393383818255	29.814667198086887	28.63092865683539
42	20.819051414906337	20.145476285372656	31.353128736548424	27.68234356317258
43	19.232762056596254	21.442805898764448	30.561976883220403	28.76245516141889
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	5.0
9	7.0
10	13.0
11	19.0
12	19.0
13	20.5
14	22.0
15	48.0
16	74.0
17	72.5
18	71.0
19	71.0
20	83.0
21	95.0
22	69.5
23	44.0
24	49.0
25	54.0
26	54.0
27	65.5
28	77.0
29	89.5
30	102.0
31	131.0
32	160.0
33	160.0
34	213.5
35	267.0
36	315.0
37	363.0
38	485.0
39	607.0
40	607.0
41	809.5
42	1012.0
43	1334.0
44	1656.0
45	2803.5
46	3951.0
47	3951.0
48	4666.5
49	5382.0
50	5934.5
51	6487.0
52	6619.5
53	6752.0
54	6752.0
55	6116.5
56	5481.0
57	5213.5
58	4946.0
59	4478.5
60	4011.0
61	4011.0
62	3571.5
63	3132.0
64	2484.0
65	1836.0
66	1574.5
67	1313.0
68	1313.0
69	1106.0
70	899.0
71	714.0
72	529.0
73	405.5
74	282.0
75	282.0
76	226.5
77	171.0
78	150.5
79	130.0
80	110.0
81	90.0
82	90.0
83	81.0
84	72.0
85	56.0
86	40.0
87	30.0
88	20.0
89	20.0
90	15.0
91	10.0
92	10.0
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	50180.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.521721801514545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2376914360905	45.38262255878836
2	7.354069958697321	8.019131127939417
3	2.9058079608172815	4.752889597449183
4	1.6594173763660953	3.618971701873256
5	0.975912862312219	2.660422479075329
6	0.6835045140538762	2.23595057791949
7	0.4934390876859534	1.8832204065364688
8	0.32530428743740636	1.4188919888401754
9	0.28144303519865493	1.3810282981267437
>10	1.886033846266311	19.83260263053009
>50	0.15716948718885923	5.876843363889996
>100	0.04020614788552213	2.9374252690314866
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	196	0.39059386209645275	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	155	0.30888800318852133	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	152	0.30290952570745316	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	149	0.296931048226385	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	144	0.2869669190912714	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	121	0.2411319250697489	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	119	0.23714627341570346	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	118	0.23515344758868076	TruSeq Adapter, Index 11 (95% over 21bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	111	0.22120366679952175	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	106	0.21123953766440814	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	103	0.20526106018333998	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	97	0.19330410522120367	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	96	0.19131127939418094	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	96	0.19131127939418094	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	93	0.1853328019131128	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	92	0.1833399760860901	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	89	0.17736149860502193	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	88	0.1753686727779992	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	84	0.16739736946990832	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	80	0.15942606616181745	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	80	0.15942606616181745	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	80	0.15942606616181745	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	79	0.15743324033479475	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	78	0.15544041450777202	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	75	0.14946193702670385	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	74	0.14746911119968115	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	72	0.1434834595456357	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	71	0.141490633718613	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	70	0.13949780789159028	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	69	0.13750498206456754	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	68	0.13551215623754484	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	68	0.13551215623754484	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	68	0.13551215623754484	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	66	0.1315265045834994	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	63	0.12554802710243124	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	62	0.12355520127540853	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	61	0.1215623754483858	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	60	0.11956954962136308	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	59	0.11757672379434038	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	58	0.11558389796731765	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	57	0.11359107214029494	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	57	0.11359107214029494	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	57	0.11359107214029494	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	56	0.11159824631327223	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	55	0.1096054204862495	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	54	0.10761259465922679	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	54	0.10761259465922679	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	53	0.10561976883220407	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	52	0.10362694300518134	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	52	0.10362694300518134	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	52	0.10362694300518134	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	52	0.10362694300518134	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	51	0.10163411717815864	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	51	0.10163411717815864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0019928258270227183	0.0	0.0	0.0	0.0
5	0.0019928258270227183	0.0	0.0	0.0	0.0
6	0.0019928258270227183	0.0	0.0	0.0	0.0
7	0.0019928258270227183	0.0	0.0	0.0	0.0
8	0.0019928258270227183	0.0	0.0	0.0	0.0
9	0.0019928258270227183	0.0	0.0	0.0	0.0
10	0.0019928258270227183	0.0	0.0	0.0	0.0
11	0.0019928258270227183	0.0	0.0	0.0	0.0
12	0.0019928258270227183	0.0	0.0	0.0	0.0
13	0.0019928258270227183	0.0	0.0	0.0	0.0
14	0.0019928258270227183	0.0	0.0	0.0	0.0
15	0.0019928258270227183	0.0	0.0	0.0	0.0
16	0.0019928258270227183	0.0	0.0	0.0	0.0
17	0.0019928258270227183	0.0	0.0	0.0	0.0
18	0.0019928258270227183	0.0	0.0	0.0	0.0
19	0.0019928258270227183	0.0	0.0	0.0	0.0
20	0.0019928258270227183	0.0	0.0	0.0	0.0
21	0.0039856516540454365	0.0	0.0	0.0039856516540454365	0.0
22	0.0039856516540454365	0.0	0.0	0.013949780789159028	0.0
23	0.0039856516540454365	0.0	0.0	0.017935432443204464	0.0
24	0.0039856516540454365	0.0	0.0	0.01992825827022718	0.0
25	0.0039856516540454365	0.0	0.0	0.01992825827022718	0.0
26	0.0039856516540454365	0.0	0.0	0.023913909924272617	0.0
27	0.0039856516540454365	0.0	0.0	0.06775607811877242	0.0
28	0.0039856516540454365	0.0	0.0	0.2391390992427262	0.0
29	0.0039856516540454365	0.0	0.0	0.5101634117178159	0.0
30	0.0039856516540454365	0.0	0.0	0.8748505380629733	0.0
31	0.0039856516540454365	0.0	0.0	1.8055001992825828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTTCT	35	5.48951E-7	37.0	15
GTCAATG	40	1.5600581E-6	32.375	31
TTCTGCG	40	1.5600581E-6	32.375	18
CTGCGGG	40	1.5600581E-6	32.375	20
CTTCTGC	40	1.5600581E-6	32.375	17
ATGAGCA	30	3.551048E-4	30.833332	35
GGTATCA	105	0.0	29.95238	1
AACGTCA	45	3.9087263E-6	28.777777	28
GGGTAAC	45	3.9087263E-6	28.777777	24
TCTGCGG	45	3.9087263E-6	28.777777	19
TCTTCTG	45	3.9087263E-6	28.777777	16
GTAACGT	45	3.9087263E-6	28.777777	26
TGCGGGT	45	3.9087263E-6	28.777777	21
GGTGCTT	45	3.9087263E-6	28.777777	10
GTGCTTC	45	3.9087263E-6	28.777777	11
TCAATGA	40	5.829341E-5	27.75	32
AATGAGC	40	5.829341E-5	27.75	34
TGAGCAA	35	8.7511813E-4	26.42857	36
CGTCAAT	50	8.866909E-6	25.899998	30
TAACGTC	50	8.866909E-6	25.899998	27
>>END_MODULE