FastQCFastQC Report
Fri 10 Feb 2017
ERR1632536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032484
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA25330.24533067824779853No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20630.1998093917193874No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15690.15196361396399363No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15550.1506076607482537No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15480.14992968414038377No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14830.14363418706730566No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13490.13065577771665227No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13400.12978409350653378No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12300.11913017538286307No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12100.1171930993603775No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12040.11661197655363183No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11530.11167243269629361No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA11060.10712030404345248No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10620.1028587367939842No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10430.10101851457262291No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT10370.10043739176587724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13550.026.8966771
TCTATAC502.7018448E-422.1999993
CGTATGC855.1793904E-821.76470631
GACCGTT1301.8189894E-1221.3461517
GTATCAA17350.020.6858792
ATACGGC2200.020.18181829
AACGACC1500.019.73333437
GCGGTAA10550.018.93838723
ACACGAA1800.018.517
CGTAGCG609.235947E-418.515
TATAGTG500.007034410718.4999985
GGTCTAT500.007034410718.4999981
GGTAATA11450.018.41921225
CGCGGTA11250.018.25333222
TAATACG10250.018.22926927
CTTATAC13200.018.21969637
GGACCGT2150.018.0697676
CGGTAAT11250.017.92444424
TAGAGAG1451.546141E-1017.8620685
CTCGTAT1054.796093E-717.61904729
Produced by FastQC (version 0.11.5)