FastQCFastQC Report
Fri 10 Feb 2017
ERR1632535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534049
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA67630.2668851312662068No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA59310.2340523012775207No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA47400.1870524208490049No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA46960.18531606926306476No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA46020.18160659087491993No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT41470.16365113697485723No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA38460.15177291362558498No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT33390.1317654078512294No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC30410.12000557211008943No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG30060.11862438334854615No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA29590.11676964415447373No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC28320.11175790207687382No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC27650.10911391216191953No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA27470.10840358651312584No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT27300.10773272340037625No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT27290.10769326086433213No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA27110.10698293521553845No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC26670.10524658362959832No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC26470.10445733290871645No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC26120.10307614414717316No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA25960.10244474357046765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25600.10102409227288028No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA25590.1009846297368362No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC25540.10078731705661571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.029.9881571
ATACGGC4550.026.83516329
ATAAGAC358.8723586E-426.428573
CTAAGTC4000.022.234
GCGGTAA27100.021.36715723
CTTATAC39850.021.16938637
CGCGGTA28250.020.56283222
CGTTATT1800.020.55555510
TAAGTCT4300.020.2209335
TTTTACG1101.7571438E-920.1818184
AGCCGCG29300.019.8890819
TACGGCT6250.019.83230
ATACACA5900.019.75423837
GGTAATA29600.019.7525
CCGCGGT29600.019.7521
CGGTAAT29450.019.72495824
TGCGGGT19350.019.59948321
AACTCCG30450.018.9556665
TGTTACG2050.018.95122116
CGTGCCA30800.018.92045410
Produced by FastQC (version 0.11.5)