FastQCFastQC Report
Fri 10 Feb 2017
ERR1632534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151950
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3480.22902270483711748No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2680.1763738071734123No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2620.17242513984863442No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2440.16057913787430075No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2370.15597235932872655No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2100.13820335636722605No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2040.13425468904244817No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1980.13030602171767028No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1880.12372490950970715No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1840.12109246462652187No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1840.12109246462652187No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT1820.11977624218492924RNA PCR Primer, Index 29 (95% over 23bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1750.11516946363935504No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1710.11253701875616978No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1650.10858835143139191No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1600.10529779532741033No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1590.104639684106614No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1540.10134912800263245No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1530.10069101678183615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCT200.001836914237.016
TTTAACG200.001836914237.03
GTTTAAC200.001836914237.02
AACGGTA200.001836914237.021
CTAACCG200.001836914237.021
TAACCGT200.001836914237.022
TTAACGG200.001836914237.04
CTTCTGA200.001836914237.01
CGGTACC200.001836914237.014
CGGTAAC200.001836914237.023
AGTCGAA200.001836914237.016
ACGGTAA200.001836914237.022
GGTATCA1800.030.8333321
AACCGTG250.00548153129.59999823
GTCGAAC250.00548153129.59999817
GCGGTAC250.00548153129.59999813
CGTGCAA250.00548153129.59999826
ATTAATC250.00548153129.5999983
ACCCTAA250.00548153129.59999818
AACGGCC250.00548153129.5999986
Produced by FastQC (version 0.11.5)