##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632534.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151950
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85472852912142	31.0	31.0	34.0	30.0	34.0
2	32.047792036854226	33.0	31.0	34.0	30.0	34.0
3	32.13698585060875	33.0	31.0	34.0	30.0	34.0
4	35.88532411977624	37.0	35.0	37.0	35.0	37.0
5	35.31238565317539	37.0	35.0	37.0	32.0	37.0
6	35.22585718986509	37.0	35.0	37.0	32.0	37.0
7	35.23913787430076	37.0	35.0	37.0	32.0	37.0
8	35.16485686080948	37.0	35.0	37.0	32.0	37.0
9	36.689068772622576	38.0	35.0	39.0	32.0	39.0
10	36.5728134254689	38.0	35.0	39.0	32.0	39.0
11	36.66933201711089	38.0	35.0	39.0	32.0	39.0
12	36.59948667324778	38.0	35.0	39.0	32.0	39.0
13	36.69046396841066	38.0	35.0	39.0	32.0	39.0
14	37.7635538005923	39.0	37.0	40.0	33.0	41.0
15	37.72135570911484	39.0	37.0	40.0	33.0	41.0
16	37.64287594603488	39.0	37.0	40.0	33.0	41.0
17	37.63934846989141	39.0	37.0	40.0	33.0	41.0
18	37.63672918723264	39.0	37.0	40.0	33.0	41.0
19	37.63517604475156	39.0	37.0	40.0	33.0	41.0
20	37.61944718657453	39.0	37.0	40.0	33.0	41.0
21	37.56204672589668	39.0	37.0	40.0	33.0	41.0
22	37.505389930898325	39.0	37.0	40.0	32.0	41.0
23	37.4241329384666	39.0	36.0	40.0	32.0	41.0
24	37.362757486015134	39.0	36.0	40.0	32.0	41.0
25	37.304383020730505	39.0	36.0	40.0	32.0	41.0
26	37.15630141493912	39.0	36.0	40.0	32.0	41.0
27	37.02048042119118	39.0	36.0	40.0	31.0	41.0
28	36.96310628496216	39.0	36.0	40.0	31.0	41.0
29	36.88089503126028	38.0	36.0	40.0	31.0	41.0
30	36.821612372490954	38.0	36.0	40.0	31.0	41.0
31	36.71973017439947	38.0	35.0	40.0	31.0	41.0
32	36.67673576834485	38.0	35.0	40.0	31.0	41.0
33	36.57028627838105	38.0	35.0	40.0	30.0	41.0
34	36.523349786113855	38.0	35.0	40.0	30.0	41.0
35	36.413037183283976	38.0	35.0	40.0	30.0	41.0
36	36.3470220467259	38.0	35.0	40.0	30.0	41.0
37	36.290793024021056	38.0	35.0	40.0	30.0	41.0
38	36.19952615992103	38.0	35.0	40.0	30.0	41.0
39	36.07722935176045	38.0	35.0	40.0	30.0	41.0
40	35.95049029285949	38.0	34.0	40.0	29.0	41.0
41	35.901316222441594	38.0	34.0	40.0	29.0	41.0
42	35.802402105955906	38.0	34.0	40.0	29.0	41.0
43	34.93095755182626	37.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	0.0
15	0.0
16	2.0
17	6.0
18	12.0
19	30.0
20	33.0
21	86.0
22	124.0
23	217.0
24	304.0
25	411.0
26	652.0
27	888.0
28	1369.0
29	1921.0
30	2585.0
31	3692.0
32	5084.0
33	7102.0
34	10987.0
35	16005.0
36	22224.0
37	29143.0
38	31956.0
39	17114.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.49489963803883	16.802895689371503	13.204343534057255	27.497861138532414
2	21.64988483053636	19.51563014149391	31.946034879894704	26.888450148075027
3	21.70187561697927	19.4814083580125	28.719315564330373	30.097400460677854
4	16.6067785455742	16.215202369200394	32.93780848963475	34.24021059559065
5	17.79993418887792	32.12767357683449	32.504113195129975	17.568279039157616
6	34.61138532411977	33.20500164527805	15.264889766370517	16.918723264231655
7	30.846331029944064	27.430733794011186	19.314906219151037	22.408028956893716
8	27.551168147416917	30.404738400789732	20.013820335636723	22.030273116156632
9	28.2329713721619	13.182625863770978	17.446528463310297	41.13787430075683
10	19.833497861138532	23.072063178677197	29.18789075353735	27.906548206646924
11	39.33135899967095	19.275419545903258	18.562685093780846	22.83053636064495
12	22.66403422178348	23.546561368871338	26.318525830865415	27.470878578479763
13	35.20500164527805	17.30898321816387	20.674564001316224	26.811451135241853
14	24.41855873642646	19.938795656465942	22.93056926620599	32.71207634090161
15	29.971043106284963	23.823626192826588	19.750575847318196	26.454754853570257
16	25.959855215531423	23.829549193813754	22.309312273774268	27.901283316880555
17	27.236590983876276	23.161566304705495	21.770319183942085	27.831523527476143
18	27.047713063507732	20.922671931556433	23.04310628496216	28.986508719973674
19	28.494899638038827	22.256663376110563	22.66271799934189	26.585718986508724
20	30.017110891740707	20.656794998354723	22.365251727541953	26.96084238236262
21	27.7143797301744	22.143468246133597	21.522869364922673	28.61928265876933
22	28.56268509378085	22.955577492596248	20.84435669628167	27.63738071734123
23	28.771964461994077	21.869035867061534	21.810463968410662	27.548535702533727
24	28.003948667324778	21.326094109904574	22.415268180322474	28.254689042448174
25	28.547548535702532	21.747285291214215	22.575189206975978	27.12997696610727
26	28.128989799276077	22.851595919710434	21.77360974004607	27.245804540967423
27	27.35373478117802	21.979598552155313	23.731490621915103	26.935176044751564
28	26.8035538005923	22.979927607765713	21.589996709443895	28.62652188219809
29	27.313589996709442	23.277393879565647	21.84995064165844	27.55906548206647
30	27.136558078315236	22.419216847647252	23.179993418887793	27.264231655149718
31	28.19019414281013	21.810463968410662	22.22507403751234	27.774267851266863
32	25.866403422178347	22.182954919381377	22.794998354721947	29.155643303718325
33	26.060546232313257	21.61237249095097	24.343534057255674	27.983547219480094
34	26.38696939782823	21.60184271141823	23.66567949983547	28.345508390918067
35	25.964461994077	22.790391576176376	24.44422507403751	26.800921355709118
36	26.204672589667656	21.367555116814742	25.039157617637382	27.388614675880223
37	26.785126686410006	20.999012833168806	24.517275419545904	27.698585060875285
38	25.52681803224745	20.227706482395526	26.145442579795986	28.10003290556104
39	25.329384666008554	19.341230667982888	27.26620598881211	28.063178677196447
40	24.627838104639686	20.254689042448174	28.3751233958539	26.742349457058246
41	22.956235603817046	19.672260612043434	29.10562685093781	28.26587693320171
42	21.65909838762751	20.29680816057914	30.343534057255678	27.700559394537677
43	21.104310628496215	21.07272128989799	29.25830865416255	28.56465942744324
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	2.0
2	0.0
3	1.5
4	3.0
5	3.0
6	8.0
7	13.0
8	11.5
9	10.0
10	16.0
11	22.0
12	22.0
13	46.0
14	70.0
15	132.0
16	194.0
17	182.5
18	171.0
19	171.0
20	215.5
21	260.0
22	241.5
23	223.0
24	267.5
25	312.0
26	312.0
27	365.0
28	418.0
29	545.0
30	672.0
31	844.0
32	1016.0
33	1016.0
34	1274.0
35	1532.0
36	1776.5
37	2021.0
38	2545.5
39	3070.0
40	3070.0
41	3705.5
42	4341.0
43	5206.5
44	6072.0
45	8260.0
46	10448.0
47	10448.0
48	12034.5
49	13621.0
50	14978.5
51	16336.0
52	16952.5
53	17569.0
54	17569.0
55	16741.5
56	15914.0
57	15145.5
58	14377.0
59	13447.0
60	12517.0
61	12517.0
62	11289.0
63	10061.0
64	8434.0
65	6807.0
66	5864.5
67	4922.0
68	4922.0
69	4200.5
70	3479.0
71	2952.5
72	2426.0
73	1868.0
74	1310.0
75	1310.0
76	1031.5
77	753.0
78	592.0
79	431.0
80	350.0
81	269.0
82	269.0
83	204.5
84	140.0
85	112.5
86	85.0
87	59.0
88	33.0
89	33.0
90	26.0
91	19.0
92	11.5
93	4.0
94	4.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	151950.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.27278710102008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.67185083347654	53.71898650871998
2	5.80963223921636	7.11944718657453
3	1.9633957724694964	3.609081934846989
4	1.0504382196253652	2.574531095755183
5	0.744328922495274	2.28035538005923
6	0.4876267399896889	1.7926949654491608
7	0.3469238700807699	1.4879894702204672
8	0.27925760439938135	1.3688713392563343
9	0.2201838803918199	1.2142152023692006
>10	1.2491407458326174	14.408028956893714
>50	0.12351778656126483	5.348469891411649
>100	0.053703385461419485	5.077328068443567
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	348	0.22902270483711748	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	268	0.1763738071734123	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	262	0.17242513984863442	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	244	0.16057913787430075	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	237	0.15597235932872655	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	210	0.13820335636722605	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	204	0.13425468904244817	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	198	0.13030602171767028	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	188	0.12372490950970715	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	184	0.12109246462652187	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	184	0.12109246462652187	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	182	0.11977624218492924	RNA PCR Primer, Index 29 (95% over 23bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	175	0.11516946363935504	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	171	0.11253701875616978	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	165	0.10858835143139191	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	160	0.10529779532741033	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	159	0.104639684106614	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	154	0.10134912800263245	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	153	0.10069101678183615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	6.581112207963146E-4	0.0
17	0.0	0.0	0.0	0.0013162224415926291	0.0
18	0.0	0.0	0.0	0.0013162224415926291	0.0
19	0.0	0.0	0.0	0.0019743336623889436	0.0
20	0.0	0.0	0.0	0.0019743336623889436	0.0
21	0.0	0.0	0.0	0.0026324448831852583	0.0
22	0.0	0.0	0.0	0.004606778545574202	0.0
23	0.0	0.0	0.0	0.004606778545574202	0.0
24	0.0	0.0	0.0	0.009871668311944718	0.0
25	0.0	0.0	0.0	0.011846001974333662	0.0
26	0.0	0.0	0.0	0.018427114182296808	0.0
27	0.0	0.0	0.0	0.0539651201052978	0.0
28	0.0	0.0	0.0	0.18427114182296808	0.0
29	0.0	0.0	0.0	0.37314906219151034	0.0
30	0.0	0.0	0.0	0.6311286607436657	0.0
31	0.0	0.0	0.0	1.3320171108917407	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCT	20	0.0018369142	37.0	16
TTTAACG	20	0.0018369142	37.0	3
GTTTAAC	20	0.0018369142	37.0	2
AACGGTA	20	0.0018369142	37.0	21
CTAACCG	20	0.0018369142	37.0	21
TAACCGT	20	0.0018369142	37.0	22
TTAACGG	20	0.0018369142	37.0	4
CTTCTGA	20	0.0018369142	37.0	1
CGGTACC	20	0.0018369142	37.0	14
CGGTAAC	20	0.0018369142	37.0	23
AGTCGAA	20	0.0018369142	37.0	16
ACGGTAA	20	0.0018369142	37.0	22
GGTATCA	180	0.0	30.833332	1
AACCGTG	25	0.005481531	29.599998	23
GTCGAAC	25	0.005481531	29.599998	17
GCGGTAC	25	0.005481531	29.599998	13
CGTGCAA	25	0.005481531	29.599998	26
ATTAATC	25	0.005481531	29.599998	3
ACCCTAA	25	0.005481531	29.599998	18
AACGGCC	25	0.005481531	29.599998	6
>>END_MODULE