FastQCFastQC Report
Fri 10 Feb 2017
ERR1632533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620382
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA55940.34522723654051946No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA47750.29468359929942445No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA45430.28036598777325344No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA37730.23284632882863426No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT37290.23013091974608454No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA36480.22513209848048177No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA35530.21926928341588586No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT28100.17341589822646758No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA28070.17323075669811192No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA26080.1609497019838532No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA25630.1581725790585183No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG23420.14453381980298474No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC23260.1435463983184212No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC22770.14052242002194543No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG22140.13663444792647658No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC22000.1357704541274835No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC21930.13533845722798699No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA21810.13459789111456433No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC21780.1344127495862087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21710.13398075268671214No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA21460.13243790661708166No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT21360.13182076818922944No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC20980.12947564216339108No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA20780.1282413653076867No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA20740.12799450993654585No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC20390.12583452543906312No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20340.12552595622513704No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20320.1254025285395666No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC20290.12521738701121093No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA20060.12379796862715088No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG19840.12244026408587605No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC19740.12182312565802386No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC19510.1204037072739638No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC19500.12034199343117857No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG19380.11960142731775594No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT18940.11688601823520625No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA18850.11633059365013929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.11546659985114621No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG18640.11503460295164966No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT18530.11435575068101227No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC18290.11287461845416698No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT18220.11244262155467044No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC17950.11077634779946952No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC17940.11071463395668428No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17890.11040606474275819No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT17710.10929521557262424No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG17560.10836950793084593No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG17250.10645637880450412No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA17130.10571581269108149No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT17070.10534552963437017No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG17060.10528381579158494No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17040.10516038810601451No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG16920.10441982199259187No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA16850.10398782509309533No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT16740.10330897282245792No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT16490.10176612675282742No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC16430.1013958436961161No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG16400.10121070216776043No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG16260.10034670836876736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30050.027.950081
GTGTAAT405.94161E-527.751
GGTTTAT400.001931526823.1251
CGTAGGG400.001931526823.1253
TTACGAG1400.022.4642856
ATACACA4450.022.44943637
ATACGGC3900.022.29487229
ATTACCT1350.021.92592633
TTTTACG1450.021.6896554
GTATACC705.104055E-621.14285731
CGTTATT2300.020.10869410
GTATCAA42050.020.0178362
TACGAGG1600.019.656257
TCAATGC2000.019.42532
TACGGCT4600.019.30434630
CTTATAC25650.019.18518437
TTTACGA1650.019.0606085
GCGGTAA23500.018.7361723
CAATGCT2100.018.533
CCTATTC701.219589E-418.53
Produced by FastQC (version 0.11.5)