##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632533.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1620382
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.023100725631366	34.0	33.0	34.0	31.0	34.0
2	33.15719688320409	34.0	33.0	34.0	31.0	34.0
3	33.22644845474709	34.0	33.0	34.0	31.0	34.0
4	36.50538268136773	37.0	37.0	37.0	35.0	37.0
5	36.480597784966754	37.0	37.0	37.0	35.0	37.0
6	36.533693289607015	37.0	37.0	37.0	35.0	37.0
7	36.541219292734674	37.0	37.0	37.0	35.0	37.0
8	36.517523028520436	37.0	37.0	37.0	35.0	37.0
9	38.29982744809557	39.0	39.0	39.0	37.0	39.0
10	38.34078445699841	39.0	39.0	39.0	37.0	39.0
11	38.40310432971978	39.0	39.0	39.0	37.0	39.0
12	38.365608850258766	39.0	39.0	39.0	37.0	39.0
13	38.39830175847423	39.0	39.0	39.0	37.0	39.0
14	39.92726653344705	41.0	40.0	41.0	38.0	41.0
15	39.93051700154655	41.0	40.0	41.0	38.0	41.0
16	39.880881174932824	41.0	40.0	41.0	38.0	41.0
17	39.89400585787796	41.0	40.0	41.0	38.0	41.0
18	39.90406459711352	41.0	40.0	41.0	38.0	41.0
19	39.8962226191108	41.0	40.0	41.0	38.0	41.0
20	39.876403835638754	41.0	40.0	41.0	38.0	41.0
21	39.83552767186997	41.0	40.0	41.0	38.0	41.0
22	39.79977128849864	41.0	40.0	41.0	38.0	41.0
23	39.771581022252775	41.0	40.0	41.0	38.0	41.0
24	39.74465527264559	41.0	40.0	41.0	38.0	41.0
25	39.7094006228161	41.0	40.0	41.0	38.0	41.0
26	39.607886288541835	41.0	40.0	41.0	37.0	41.0
27	39.50285673378253	41.0	39.0	41.0	37.0	41.0
28	39.47140427380704	41.0	39.0	41.0	37.0	41.0
29	39.43660136930674	41.0	39.0	41.0	37.0	41.0
30	39.384530931595144	41.0	39.0	41.0	36.0	41.0
31	39.31622728467732	41.0	39.0	41.0	36.0	41.0
32	39.27369904133717	41.0	39.0	41.0	36.0	41.0
33	39.203071868238474	41.0	39.0	41.0	35.0	41.0
34	39.16459267012346	41.0	39.0	41.0	35.0	41.0
35	39.08707514647781	41.0	39.0	41.0	35.0	41.0
36	39.01393745425462	40.0	39.0	41.0	35.0	41.0
37	38.95786240528468	40.0	39.0	41.0	35.0	41.0
38	38.88483950080907	40.0	39.0	41.0	35.0	41.0
39	38.772619049088426	40.0	38.0	41.0	35.0	41.0
40	38.69105248021763	40.0	38.0	41.0	35.0	41.0
41	38.61455014928578	40.0	38.0	41.0	35.0	41.0
42	38.52797488493454	40.0	38.0	41.0	35.0	41.0
43	37.78561783579428	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	0.0
15	2.0
16	1.0
17	0.0
18	7.0
19	30.0
20	68.0
21	140.0
22	317.0
23	594.0
24	966.0
25	1337.0
26	1945.0
27	2883.0
28	3911.0
29	5591.0
30	7446.0
31	9851.0
32	12878.0
33	17192.0
34	25991.0
35	41555.0
36	63350.0
37	125952.0
38	334306.0
39	964066.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.68247734176262	16.125333409035647	12.475206463661038	27.716982785540694
2	22.004687783497964	19.633641943689824	32.01782048924266	26.34384978356955
3	22.736367103559534	19.37839348993015	28.555735622834614	29.329503783675705
4	15.820528739519446	16.439518582655204	34.64713876110695	33.092813916718406
5	18.346599752404064	32.06206931451966	31.695674229903815	17.89565670317246
6	32.13026311079733	33.92434623440646	16.09743875209673	17.84795190269949
7	29.361594981924018	27.617808640184844	19.266753148331688	23.75384322955945
8	26.457279826608787	30.194793573367267	20.577370027561404	22.770556572462546
9	27.92693327869601	13.04710864475167	17.719525395863446	41.30643268068888
10	20.46813652583156	22.12984345666639	28.611710078240808	28.79030993926124
11	39.082265786709556	20.003678145029998	18.907578583321712	22.006477484938735
12	22.203529784951943	23.510814116671256	27.039673360972905	27.245982737403896
13	35.797978501365726	17.868811181560893	20.492390066046156	25.840820251027225
14	23.98878782904278	20.689504079902147	23.37029169664931	31.95141639440576
15	30.559707525756274	23.545559010159334	19.81569777990622	26.079035684178177
16	24.660975004659395	23.62757670722089	23.307467004693954	28.40398128342576
17	26.435618267791178	23.31567494578439	22.08868032352865	28.160026462895786
18	26.571080152704734	20.889086647469547	23.541115613478798	28.998717586346924
19	28.331652659681485	22.53820395437619	23.283028322951008	25.84711506299132
20	30.064453937404885	20.462335424609755	22.75562182250852	26.717588815476844
21	27.844607012420532	21.758079267728228	22.081891800822277	28.31542191902897
22	27.889349548439814	23.265871874656714	21.463210526900447	27.381568050003025
23	28.49871203210107	21.59188388910763	22.454396555873863	27.455007522917434
24	27.92421786961346	21.081880692330575	23.00377318434789	27.990128253708075
25	28.443971853550583	22.43557383382437	22.83424525821689	26.28620905440816
26	28.318631038853802	22.708842729677322	22.39206557466079	26.580460656808086
27	27.455254378288572	21.808067480384256	24.54044786969986	26.196230271627307
28	26.411364727576586	23.620788184514517	21.775544285236446	28.192302802672458
29	27.480063343088233	23.65287938276283	21.764620935063462	27.10243633908547
30	26.878538517460697	22.49753453198073	24.219721028745074	26.4042059218135
31	28.332208084266547	21.67544443223882	22.61886394689647	27.373483536598158
32	25.087479372148046	22.013142579959542	23.424661592143085	29.474716455749324
33	26.03083717296292	21.693588302017673	24.588831522443474	27.686743002575938
34	25.401294262710895	21.77301401768225	24.509158951407755	28.316532768199103
35	26.027751480823657	23.09708451463914	24.411157369064824	26.464006635472376
36	26.211473590795258	21.562940096841363	25.35056548394144	26.875020828421942
37	26.901928063876294	20.912476193885148	24.643941984050674	27.541653758187884
38	25.65524672577207	20.250471802328093	26.079961391819957	28.01432008007988
39	24.861730135239714	19.10950627691495	27.799123910287822	28.229639677557515
40	24.761630282242088	20.562003280707884	28.34115659147041	26.33520984557962
41	23.085914309095017	19.668571978706257	29.3687537876871	27.876759924511628
42	21.44056154659827	20.603228127688407	30.434798707958986	27.52141161775433
43	20.68586296317782	21.739565114892663	29.197868157014828	28.37670376491469
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	16.5
2	16.0
3	78.5
4	141.0
5	141.0
6	164.5
7	188.0
8	206.0
9	224.0
10	346.5
11	469.0
12	469.0
13	966.5
14	1464.0
15	2465.5
16	3467.0
17	3252.0
18	3037.0
19	3037.0
20	3605.0
21	4173.0
22	3237.5
23	2302.0
24	2346.0
25	2390.0
26	2390.0
27	2712.0
28	3034.0
29	3651.5
30	4269.0
31	5010.5
32	5752.0
33	5752.0
34	8403.0
35	11054.0
36	11579.0
37	12104.0
38	17919.5
39	23735.0
40	23735.0
41	31778.5
42	39822.0
43	50600.5
44	61379.0
45	91872.5
46	122366.0
47	122366.0
48	145930.0
49	169494.0
50	185023.5
51	200553.0
52	204950.0
53	209347.0
54	209347.0
55	197139.5
56	184932.0
57	174842.0
58	164752.0
59	146327.0
60	127902.0
61	127902.0
62	112129.5
63	96357.0
64	75951.5
65	55546.0
66	48045.5
67	40545.0
68	40545.0
69	34254.0
70	27963.0
71	22993.0
72	18023.0
73	13788.5
74	9554.0
75	9554.0
76	7774.5
77	5995.0
78	4772.5
79	3550.0
80	2811.0
81	2072.0
82	2072.0
83	1656.5
84	1241.0
85	928.5
86	616.0
87	457.0
88	298.0
89	298.0
90	230.0
91	162.0
92	110.0
93	58.0
94	35.0
95	12.0
96	12.0
97	9.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1620382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.52437247708225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07465684717309	24.232027396846476
2	8.514952511727213	5.027972591618026
3	2.647240576925702	2.344743504888016
4	1.3394622629667956	1.581871310833087
5	0.8684177651976636	1.2819744782705587
6	0.5688903048545582	1.0077677555476108
7	0.43278447234853507	0.8944382974740958
8	0.3617106579087051	0.8543424154421669
9	0.2706816881016386	0.7192536284014363
>10	2.1965512642827116	13.316934428668276
>50	0.33570877310907876	7.015665384517758
>100	0.3182259891516261	19.137829633595654
>500	0.04461898775466528	9.215281533332451
>1k	0.025887431574640702	13.022291859770881
>5k	2.1046692337106264E-4	0.34760578079353294
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	5594	0.34522723654051946	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	4775	0.29468359929942445	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	4543	0.28036598777325344	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	3773	0.23284632882863426	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	3729	0.23013091974608454	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	3648	0.22513209848048177	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	3553	0.21926928341588586	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	2810	0.17341589822646758	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	2807	0.17323075669811192	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2608	0.1609497019838532	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	2563	0.1581725790585183	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	2342	0.14453381980298474	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	2326	0.1435463983184212	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	2277	0.14052242002194543	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	2214	0.13663444792647658	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	2200	0.1357704541274835	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	2193	0.13533845722798699	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	2181	0.13459789111456433	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	2178	0.1344127495862087	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2171	0.13398075268671214	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	2146	0.13243790661708166	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	2136	0.13182076818922944	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	2098	0.12947564216339108	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2078	0.1282413653076867	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	2074	0.12799450993654585	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	2039	0.12583452543906312	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2034	0.12552595622513704	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2032	0.1254025285395666	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	2029	0.12521738701121093	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2006	0.12379796862715088	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	1984	0.12244026408587605	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	1974	0.12182312565802386	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	1951	0.1204037072739638	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	1950	0.12034199343117857	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	1938	0.11960142731775594	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	1894	0.11688601823520625	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	1885	0.11633059365013929	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1871	0.11546659985114621	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	1864	0.11503460295164966	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	1853	0.11435575068101227	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	1829	0.11287461845416698	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1822	0.11244262155467044	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	1795	0.11077634779946952	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1794	0.11071463395668428	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1789	0.11040606474275819	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1771	0.10929521557262424	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	1756	0.10836950793084593	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1725	0.10645637880450412	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	1713	0.10571581269108149	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1707	0.10534552963437017	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	1706	0.10528381579158494	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1704	0.10516038810601451	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	1692	0.10441982199259187	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	1685	0.10398782509309533	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	1674	0.10330897282245792	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1649	0.10176612675282742	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	1643	0.1013958436961161	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	1640	0.10121070216776043	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	1626	0.10034670836876736	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.8514152835565935E-4	0.0	0.0	0.0	0.0
9	1.8514152835565935E-4	0.0	0.0	0.0	0.0
10	1.8514152835565935E-4	0.0	0.0	0.0	0.0
11	1.8514152835565935E-4	0.0	0.0	0.0	0.0
12	1.8514152835565935E-4	0.0	0.0	0.0	0.0
13	1.8514152835565935E-4	0.0	0.0	0.0	0.0
14	1.8514152835565935E-4	0.0	0.0	2.4685537114087913E-4	0.0
15	1.8514152835565935E-4	0.0	0.0	8.63993798993077E-4	0.0
16	1.8514152835565935E-4	0.0	0.0	0.0016662737552009341	0.0
17	1.8514152835565935E-4	0.0	0.0	0.002591981396979231	6.171384278521978E-5
18	2.4685537114087913E-4	0.0	0.0	0.003147405982046209	6.171384278521978E-5
19	2.4685537114087913E-4	0.0	0.0	0.0038879720954688463	6.171384278521978E-5
20	2.4685537114087913E-4	0.0	0.0	0.005060535108388022	6.171384278521978E-5
21	2.4685537114087913E-4	0.0	0.0	0.007405661134226374	6.171384278521978E-5
22	2.4685537114087913E-4	0.0	0.0	0.011046777858554341	6.171384278521978E-5
23	2.4685537114087913E-4	0.0	0.0	0.013391903884392693	6.171384278521978E-5
24	2.4685537114087913E-4	0.0	0.0	0.01697130676593544	6.171384278521978E-5
25	2.4685537114087913E-4	0.0	0.0	0.01894614973506247	6.171384278521978E-5
26	2.4685537114087913E-4	0.0	0.0	0.024685537114087913	6.171384278521978E-5
27	2.4685537114087913E-4	0.0	0.0	0.06449096571055467	6.171384278521978E-5
28	3.702830567113187E-4	0.0	0.0	0.21143162538216298	6.171384278521978E-5
29	3.702830567113187E-4	0.0	0.0	0.42471466604788255	6.171384278521978E-5
30	4.937107422817583E-4	0.0	0.0	0.7122394595842215	6.171384278521978E-5
31	4.937107422817583E-4	0.0	0.0	1.486501331167589	6.171384278521978E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3005	0.0	27.95008	1
GTGTAAT	40	5.94161E-5	27.75	1
GGTTTAT	40	0.0019315268	23.125	1
CGTAGGG	40	0.0019315268	23.125	3
TTACGAG	140	0.0	22.464285	6
ATACACA	445	0.0	22.449436	37
ATACGGC	390	0.0	22.294872	29
ATTACCT	135	0.0	21.925926	33
TTTTACG	145	0.0	21.689655	4
GTATACC	70	5.104055E-6	21.142857	31
CGTTATT	230	0.0	20.108694	10
GTATCAA	4205	0.0	20.017836	2
TACGAGG	160	0.0	19.65625	7
TCAATGC	200	0.0	19.425	32
TACGGCT	460	0.0	19.304346	30
CTTATAC	2565	0.0	19.185184	37
TTTACGA	165	0.0	19.060608	5
GCGGTAA	2350	0.0	18.73617	23
CAATGCT	210	0.0	18.5	33
CCTATTC	70	1.219589E-4	18.5	3
>>END_MODULE