FastQCFastQC Report
Fri 10 Feb 2017
ERR1632532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147074
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4520.30732828372112No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3850.2617729850279451No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3210.21825747582849447No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3210.21825747582849447No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3160.21485782667228742No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3140.2134979670098046No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3100.2107782476848389No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2980.2026190897099419No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2820.19174021241007927No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2810.19106028257883786No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2800.19038035274759646No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2670.18154126494145806No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT2660.18086133511021663TruSeq Adapter, Index 6 (95% over 21bp)
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2650.1801814052789752No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2610.17746168595400955No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2350.15978351034173274No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2210.1502644927043529No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2150.14618491371690442No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2020.13734582591076602No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2000.13598596624828319No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1980.13462610658580035No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1930.13122645742959327No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1930.13122645742959327No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1900.12918666793586903No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1890.12850673810462762No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1880.1278268082733862No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1860.12646694861090335No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1780.12102750996097203No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1770.12034758012973061No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1750.11898772046724777No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1750.11898772046724777No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1740.11830779063600637No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1710.11626800114228211No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1700.11558807131104071No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1660.11286835198607503No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1590.10810884316738512No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1570.1067489835049023No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1560.10606905367366087No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1550.10538912384241945No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1520.10334933434869521No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1510.10266940451745381No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1500.10198947468621239No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1480.10062961502372955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2550.030.4705891
GTGAGTA250.00548096229.62
CCAGTAC405.9055074E-527.7531
AGTACCG358.832509E-426.42857233
TACCGTG358.832509E-426.42857235
TTATACA1000.025.90000237
CAGTACC451.3155617E-424.66666632
GTATCAA3300.023.5454542
GCGAGAT400.001923232823.12518
GATCAGG400.001923232823.1251
ACGGGTC400.001923232823.12517
GCCGTCT400.001923232823.12536
GCGTAAT400.001923232823.1251
GTCCTGC603.6979207E-521.5833321
GGTAATA1700.020.6764725
GCGGTAA1700.020.6764723
GTACCGT450.003810199720.55555534
CAGCCTT450.003810199720.55555525
ATCAGGA450.003810199720.5555552
ACCGTGA450.003810199720.55555536
Produced by FastQC (version 0.11.5)