FastQCFastQC Report
Fri 10 Feb 2017
ERR1632531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54364
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2810.5168861746744169No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1710.3145463909940402No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1590.29247296004709No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1550.2851151497314399No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1470.2703995291001398No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1460.26856007652122726No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1450.26672062394231477No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1410.2593628136266647No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1410.2593628136266647No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1380.25384445588992716No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1340.24648664557427707No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1210.2225737620484144No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1190.21889485689058938No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1120.20601868883820176No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1110.20417923625928924No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1060.19498197336472667No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1030.1894636156279891No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT970.178426900154514Illumina PCR Primer Index 10 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC940.17290854241777648No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT920.16922963725995144No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT910.16739018468103892No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA900.16555073210212642No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT840.15451401662865133No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA790.14531675373408873No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA730.13428003826061363No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA720.13244058568170114No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC710.1306011331027886No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC690.12692222794496358No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC680.12508277536605106No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA680.12508277536605106No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA650.11956441762931351No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT650.11956441762931351No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG640.117724965050401No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC620.11404605989257598No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG620.11404605989257598No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC610.11220660731366344No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC600.11036715473475094No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG600.11036715473475094No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA580.10668824957692591No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC570.10484879699801339No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTTAT560.10300934441910088No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC560.10300934441910088No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA550.10116989184018835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCCT200.001826551937.014
CCCCTCG200.001826551937.016
TTACGAG200.001826551937.01
AATGAGC251.2182182E-436.99999634
GGTATCA1100.035.318181
GAGGCCA352.3470095E-531.7142875
TCAATGA352.3470095E-531.71428732
CAATGAG303.5549433E-430.83333233
AGGCCAA303.5549433E-430.8333326
TGAGCAA303.5549433E-430.83333236
ATGAGCA303.5549433E-430.83333235
GGCATGC250.00545096229.59999822
TCGGCAT250.00545096229.59999820
CCGCCCC250.00545096229.59999813
TACGAGG250.00545096229.5999982
ACCTTTG502.641027E-729.5999987
ACCTTGG250.00545096229.59999829
GCACCTT250.00545096229.59999827
TACCTTT502.641027E-729.5999986
ATACCTT502.641027E-729.5999985
Produced by FastQC (version 0.11.5)