##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632531.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54364
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02529247296005	34.0	33.0	34.0	31.0	34.0
2	33.17171289824148	34.0	33.0	34.0	31.0	34.0
3	33.22857037745567	34.0	33.0	34.0	31.0	34.0
4	36.516279155323375	37.0	37.0	37.0	35.0	37.0
5	36.495235817820614	37.0	37.0	37.0	35.0	37.0
6	36.54304319034655	37.0	37.0	37.0	35.0	37.0
7	36.545949525421236	37.0	37.0	37.0	35.0	37.0
8	36.5182657641086	37.0	37.0	37.0	35.0	37.0
9	38.32747774262379	39.0	39.0	39.0	37.0	39.0
10	38.3563939371643	39.0	39.0	39.0	37.0	39.0
11	38.4177948642484	39.0	39.0	39.0	37.0	39.0
12	38.371293503053494	39.0	39.0	39.0	37.0	39.0
13	38.40418291516445	39.0	39.0	39.0	37.0	39.0
14	39.92060922669413	41.0	40.0	41.0	38.0	41.0
15	39.92736001765874	41.0	40.0	41.0	38.0	41.0
16	39.8736480023545	41.0	40.0	41.0	38.0	41.0
17	39.89379000809359	41.0	40.0	41.0	38.0	41.0
18	39.92141858582886	41.0	40.0	41.0	38.0	41.0
19	39.90550732102126	41.0	40.0	41.0	38.0	41.0
20	39.88834522846001	41.0	40.0	41.0	38.0	41.0
21	39.83980207490251	41.0	40.0	41.0	38.0	41.0
22	39.812706938415126	41.0	40.0	41.0	38.0	41.0
23	39.77352659848429	41.0	40.0	41.0	38.0	41.0
24	39.764237362960785	41.0	40.0	41.0	38.0	41.0
25	39.703369877124565	41.0	40.0	41.0	38.0	41.0
26	39.625193142520786	41.0	40.0	41.0	37.0	41.0
27	39.51699654182915	41.0	39.0	41.0	37.0	41.0
28	39.48539474652343	41.0	39.0	41.0	37.0	41.0
29	39.418677801486275	41.0	39.0	41.0	37.0	41.0
30	39.38462953425061	41.0	39.0	41.0	36.0	41.0
31	39.32065337355603	41.0	39.0	41.0	36.0	41.0
32	39.277867706570525	41.0	39.0	41.0	36.0	41.0
33	39.19816422632625	41.0	39.0	41.0	35.0	41.0
34	39.1403870208226	41.0	39.0	41.0	35.0	41.0
35	39.05790596718417	41.0	39.0	41.0	35.0	41.0
36	39.02271723934957	41.0	39.0	41.0	35.0	41.0
37	38.9680303141785	40.0	39.0	41.0	35.0	41.0
38	38.89382679714517	40.0	39.0	41.0	35.0	41.0
39	38.7846736811125	40.0	38.0	41.0	35.0	41.0
40	38.702836435876684	40.0	38.0	41.0	35.0	41.0
41	38.661779854315355	40.0	38.0	41.0	35.0	41.0
42	38.58424692811419	40.0	38.0	41.0	35.0	41.0
43	37.87951585608123	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	8.0
22	9.0
23	22.0
24	35.0
25	46.0
26	74.0
27	111.0
28	128.0
29	190.0
30	249.0
31	330.0
32	436.0
33	572.0
34	877.0
35	1332.0
36	2118.0
37	4145.0
38	10709.0
39	32969.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.754396291663596	15.190199396659555	13.302921050695312	28.75248326098153
2	22.50018394525789	18.80656316680156	30.956147450518724	27.73710543742182
3	21.591494371275108	18.44051210359797	29.017364432344934	30.950629092781988
4	15.45140166286513	16.205577220219265	34.55043778971378	33.792583327201825
5	17.046207048782282	32.31366345375616	32.817673460378195	17.822456037083363
6	32.39643881980723	33.93422117577809	15.942535501434774	17.726804502979913
7	29.289603414023986	27.093297034802443	19.667426973732617	23.949672577440953
8	27.05466853064528	28.98609373850342	21.093002722389816	22.866235008461484
9	27.746302700316384	11.829519534986389	17.143698035464645	43.28047972923258
10	19.963578838937533	21.998013391214773	27.593628136266645	30.44477963358105
11	40.33183724523582	18.823118240011773	17.399381943933484	23.44566257081892
12	22.28680744610404	23.83746597012729	26.061364138032523	27.81436244573615
13	38.02332425870061	16.529320874107867	19.027297476271062	26.42005739092046
14	24.291810757118682	19.96909719667427	22.836803767198884	32.90228827900817
15	31.169523949672577	22.96740490030167	18.82127878743286	27.041792362592894
16	25.0	23.703185931866678	22.299683614156425	28.997130453976894
17	28.294459568832313	23.155029063350746	20.6349790302406	27.915532337576337
18	27.571554705319695	19.468766095210064	22.1635641233169	30.796115076153335
19	29.320874107865496	22.00721065410934	22.32911485541903	26.342800382606136
20	32.34125524243985	19.065925980428226	21.078287101758516	27.514531675373412
21	28.66235008461482	20.66992862923994	20.71591494371275	29.95180634243249
22	29.475388124494152	21.955705981899786	19.941505407990583	28.62740048561548
23	29.688764623648	20.588992715767787	20.899860201604003	28.822382458980204
24	28.44897358546097	19.601206680891767	22.393495695680965	29.556324037966302
25	29.526892796703702	21.184975351335442	21.736811125009197	27.551320726951662
26	29.149805018026637	21.880288426164373	21.27694798028107	27.69295857552792
27	26.701493635494078	20.706717680818187	24.827091457582224	27.76469722610551
28	26.6021631962328	22.386137885365315	21.205209329703482	29.806489588698405
29	27.70767419615922	23.83746597012729	21.017585166654403	27.437274667059082
30	27.40600397321757	20.791332499448163	23.89448900007358	27.908174527260687
31	28.658671179456995	21.9869766757413	21.948348171584136	27.40600397321757
32	26.03929070708557	20.769259068501214	22.766904569200204	30.42454565521301
33	26.3961445073946	20.989993377970716	24.49598999337797	28.117872121256717
34	26.43109410639394	20.93480980060334	23.791479655654477	28.842616437348244
35	24.996321094842173	23.042822456037083	24.77558678537267	27.18526966374807
36	26.74747994996689	21.074608196600693	24.957692590685014	27.22021926274741
37	26.638952247811055	20.78765359429034	24.851004341108084	27.72238981679052
38	25.413876830255315	19.55154146126113	26.69781473033625	28.336766978147303
39	25.145316753734086	18.26760356118019	27.746302700316384	28.840776984769334
40	24.175925244647193	19.663748068574794	29.572879111176515	26.5874475756015
41	22.658376867044367	18.613420646015747	29.69796188654257	29.030240600397324
42	20.292840850562875	19.761239055257153	32.37252593628136	27.573394157898612
43	19.14134353616364	20.989993377970716	30.43926127584431	29.42940181002134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	2.5
9	4.0
10	5.5
11	7.0
12	7.0
13	12.0
14	17.0
15	34.0
16	51.0
17	52.0
18	53.0
19	53.0
20	69.5
21	86.0
22	75.0
23	64.0
24	56.5
25	49.0
26	49.0
27	55.5
28	62.0
29	80.0
30	98.0
31	112.5
32	127.0
33	127.0
34	182.5
35	238.0
36	290.5
37	343.0
38	465.0
39	587.0
40	587.0
41	788.5
42	990.0
43	1323.5
44	1657.0
45	2803.5
46	3950.0
47	3950.0
48	4575.5
49	5201.0
50	5970.5
51	6740.0
52	6888.5
53	7037.0
54	7037.0
55	6478.0
56	5919.0
57	5592.0
58	5265.0
59	5017.5
60	4770.0
61	4770.0
62	4423.5
63	4077.0
64	3236.5
65	2396.0
66	2003.0
67	1610.0
68	1610.0
69	1401.5
70	1193.0
71	985.0
72	777.0
73	594.5
74	412.0
75	412.0
76	334.5
77	257.0
78	199.0
79	141.0
80	118.5
81	96.0
82	96.0
83	68.5
84	41.0
85	31.5
86	22.0
87	17.0
88	12.0
89	12.0
90	7.0
91	2.0
92	5.5
93	9.0
94	4.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	54364.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.526524906187916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.71549086863818	51.61871826944301
2	5.889805630233924	7.01199323081451
3	2.2619820153888943	4.039437863291885
4	1.2422360248447204	2.957839746891325
5	0.7169123327462068	2.133764991538518
6	0.5376842495596551	1.9203884923846664
7	0.4078983962176694	1.6996541829151643
8	0.3399153301813912	1.6187182694430136
9	0.2193998949352616	1.1754101979250975
>10	1.501807731528692	16.92480317857406
>50	0.11433515651555885	4.4294018100213375
>100	0.05253236920985137	4.469869766757413
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	281	0.5168861746744169	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	171	0.3145463909940402	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	159	0.29247296004709	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	155	0.2851151497314399	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	147	0.2703995291001398	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	146	0.26856007652122726	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	145	0.26672062394231477	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	141	0.2593628136266647	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	141	0.2593628136266647	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	138	0.25384445588992716	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	134	0.24648664557427707	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	121	0.2225737620484144	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	119	0.21889485689058938	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	112	0.20601868883820176	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	111	0.20417923625928924	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	106	0.19498197336472667	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	103	0.1894636156279891	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	97	0.178426900154514	Illumina PCR Primer Index 10 (95% over 21bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	94	0.17290854241777648	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	92	0.16922963725995144	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	91	0.16739018468103892	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	90	0.16555073210212642	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	84	0.15451401662865133	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	79	0.14531675373408873	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	73	0.13428003826061363	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	72	0.13244058568170114	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	71	0.1306011331027886	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	69	0.12692222794496358	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	68	0.12508277536605106	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	68	0.12508277536605106	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	65	0.11956441762931351	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	65	0.11956441762931351	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	64	0.117724965050401	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	62	0.11404605989257598	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	62	0.11404605989257598	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	61	0.11220660731366344	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	60	0.11036715473475094	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	60	0.11036715473475094	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	58	0.10668824957692591	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	57	0.10484879699801339	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTTAT	56	0.10300934441910088	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	56	0.10300934441910088	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	55	0.10116989184018835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0018394525789125157	0.0
20	0.0	0.0	0.0	0.005518357736737547	0.0
21	0.0	0.0	0.0	0.01287616805238761	0.0
22	0.0	0.0	0.0	0.018394525789125156	0.0
23	0.0	0.0	0.0	0.020233978368037673	0.0
24	0.0	0.0	0.0	0.02943124126260025	0.0
25	0.0	0.0	0.0	0.031270693841512764	0.0
26	0.0	0.0	0.0	0.038628504157162825	0.0
27	0.0	0.0	0.0	0.08093591347215069	0.0
28	0.0	0.0	0.0	0.23912883525862702	0.0
29	0.0	0.0	0.0	0.5389596056213671	0.0
30	0.0	0.0	0.0	1.0006622029284085	0.0
31	0.0	0.0	0.0	1.8486498418070783	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCCCT	20	0.0018265519	37.0	14
CCCCTCG	20	0.0018265519	37.0	16
TTACGAG	20	0.0018265519	37.0	1
AATGAGC	25	1.2182182E-4	36.999996	34
GGTATCA	110	0.0	35.31818	1
GAGGCCA	35	2.3470095E-5	31.714287	5
TCAATGA	35	2.3470095E-5	31.714287	32
CAATGAG	30	3.5549433E-4	30.833332	33
AGGCCAA	30	3.5549433E-4	30.833332	6
TGAGCAA	30	3.5549433E-4	30.833332	36
ATGAGCA	30	3.5549433E-4	30.833332	35
GGCATGC	25	0.005450962	29.599998	22
TCGGCAT	25	0.005450962	29.599998	20
CCGCCCC	25	0.005450962	29.599998	13
TACGAGG	25	0.005450962	29.599998	2
ACCTTTG	50	2.641027E-7	29.599998	7
ACCTTGG	25	0.005450962	29.599998	29
GCACCTT	25	0.005450962	29.599998	27
TACCTTT	50	2.641027E-7	29.599998	6
ATACCTT	50	2.641027E-7	29.599998	5
>>END_MODULE