FastQCFastQC Report
Fri 10 Feb 2017
ERR1632530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214173
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4380.2045075709823367No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3330.15548178341807792No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2510.11719497789170437No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2370.11065820621646985No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2260.10552217132878561No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2230.10412143454123536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTT200.001838594237.014
GGTATCA2600.029.8846151
TTGAGTT250.00548648529.64
CCTTTGA358.845751E-426.428571
TAGGACA701.9077561E-723.7857134
GTACTGG652.6659563E-622.769231
TTAGGAC903.785317E-922.611113
ATCTAAG502.6921657E-422.232
CATCTAA502.6921657E-422.231
GTATCAA3550.021.8873252
CTTATAC2900.021.68965537
GCTTAGG705.0700073E-621.1428571
TAGCGCA1001.2751116E-820.354
TTCTCGT555.124337E-420.18181811
CGGTAAT1850.020.024
TGAAACA656.8686684E-519.92307726
GGTAATA2050.019.85365725
ATAGCGC851.235654E-619.5882363
GCGGTAA1900.019.47368423
CATAGCG951.6602644E-719.4736842
Produced by FastQC (version 0.11.5)