##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632530.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	214173
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07787629626517	34.0	33.0	34.0	31.0	34.0
2	33.2138691618458	34.0	33.0	34.0	31.0	34.0
3	33.282962838453024	34.0	34.0	34.0	31.0	34.0
4	36.5608503406125	37.0	37.0	37.0	35.0	37.0
5	36.52614475213962	37.0	37.0	37.0	35.0	37.0
6	36.5791066100769	37.0	37.0	37.0	35.0	37.0
7	36.57360638362445	37.0	37.0	37.0	35.0	37.0
8	36.53989998739337	37.0	37.0	37.0	35.0	37.0
9	38.33536907079791	39.0	39.0	39.0	37.0	39.0
10	38.375388120818215	39.0	39.0	39.0	37.0	39.0
11	38.44891746391936	39.0	39.0	39.0	37.0	39.0
12	38.408384810410276	39.0	39.0	39.0	37.0	39.0
13	38.43585792793676	39.0	39.0	39.0	37.0	39.0
14	40.01304552861472	41.0	40.0	41.0	38.0	41.0
15	40.0048792331433	41.0	40.0	41.0	38.0	41.0
16	39.976346224780904	41.0	40.0	41.0	38.0	41.0
17	39.97617813636639	41.0	40.0	41.0	38.0	41.0
18	39.967699009679095	41.0	40.0	41.0	38.0	41.0
19	39.97487545115397	41.0	40.0	41.0	38.0	41.0
20	39.943886484290736	41.0	40.0	41.0	38.0	41.0
21	39.913443804774644	41.0	40.0	41.0	38.0	41.0
22	39.88567653252277	41.0	40.0	41.0	38.0	41.0
23	39.841058396716676	41.0	40.0	41.0	38.0	41.0
24	39.83355978578065	41.0	40.0	41.0	38.0	41.0
25	39.777805792513526	41.0	40.0	41.0	38.0	41.0
26	39.70443986870427	41.0	40.0	41.0	38.0	41.0
27	39.63134008488465	41.0	40.0	41.0	37.0	41.0
28	39.58413992426683	41.0	40.0	41.0	37.0	41.0
29	39.54118399611529	41.0	40.0	41.0	37.0	41.0
30	39.501561821518116	41.0	40.0	41.0	37.0	41.0
31	39.434849397449725	41.0	39.0	41.0	37.0	41.0
32	39.387541847011526	41.0	39.0	41.0	36.0	41.0
33	39.32550788381355	41.0	39.0	41.0	36.0	41.0
34	39.282365190757005	41.0	39.0	41.0	36.0	41.0
35	39.216231737894134	41.0	39.0	41.0	35.0	41.0
36	39.164885396385166	41.0	39.0	41.0	35.0	41.0
37	39.105017906085266	41.0	39.0	41.0	35.0	41.0
38	39.02253318578906	41.0	39.0	41.0	35.0	41.0
39	38.93502448954817	41.0	39.0	41.0	35.0	41.0
40	38.86282117727258	40.0	39.0	41.0	35.0	41.0
41	38.80806637624724	40.0	38.0	41.0	35.0	41.0
42	38.73785211020997	40.0	38.0	41.0	35.0	41.0
43	38.027888669440124	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	15.0
21	21.0
22	54.0
23	70.0
24	132.0
25	188.0
26	220.0
27	316.0
28	496.0
29	613.0
30	870.0
31	1155.0
32	1535.0
33	2035.0
34	3132.0
35	4893.0
36	8086.0
37	15251.0
38	40478.0
39	134606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.634818581240395	16.204190070643826	12.299869731478758	27.861121616637018
2	21.32901906402768	18.87726277355222	31.94567008913355	27.848048073286545
3	21.45555228716972	19.36892138598236	28.33970668571669	30.835819641131234
4	16.27842912038399	14.853412895182865	32.395773510199696	36.47238447423345
5	17.653952645758338	32.91731450743091	32.436861789301176	16.991871057509584
6	36.4182226517815	32.37056024802379	14.498092663407618	16.713124436787083
7	31.08888608741531	26.969319195230025	19.34184047475639	22.599954242598276
8	27.961974665340634	30.917529287071666	19.336237527606187	21.78425851998151
9	27.764003866033534	13.109028682420288	16.92463569170717	42.202331759839005
10	19.359583140732024	23.323668249499235	29.765656735442843	27.551091874325895
11	39.98123012704682	19.00426290895678	18.505133700326372	22.509373263670025
12	23.576267783520798	22.950138439485837	26.09946165016132	27.374132126832045
13	34.27789684040472	17.240735293431012	21.096496757294336	27.384871108869934
14	24.68098219663543	18.741858217422365	22.10315959528045	34.47399999066175
15	29.295476087088474	23.99275352168574	19.656072427430164	27.055697963795623
16	27.213514308526282	23.32320133723672	21.431272849518848	28.032011504718145
17	27.436231457746775	23.018307629813282	21.508313372834113	28.037147539605833
18	26.669094610431753	21.61757084226303	23.178458535856528	28.53487601144869
19	28.746387266368778	21.910324830861033	22.738160272303233	26.605127630466956
20	29.394461486742024	20.839695012910123	22.72415290442773	27.04169059592012
21	27.855518669486816	22.033589668165455	21.495706741746158	28.61518492060157
22	28.51619952094802	22.34595397178916	20.95082013138911	28.187026375873707
23	28.28694560005229	22.32727748128849	21.96355282878794	27.42222408987127
24	28.708100460842402	21.64932087611417	21.37477646575432	28.267802197289104
25	28.815023368958737	21.81274016799503	22.311402464362924	27.060833998683307
26	28.523670117148285	22.148450084744574	21.897251287510564	27.430628510596573
27	28.145471184509717	21.50271042568391	22.96554654414889	27.386271845657483
28	27.057098700583172	22.460347476105767	21.9929683013265	28.489585521984562
29	27.777077409384006	23.04305397972667	21.77445336246866	27.405415248420667
30	27.275146727178495	22.35202383120188	22.956208298898552	27.41662114272107
31	28.198699182436627	22.13490962913159	22.142847137594373	27.523544050837405
32	26.015884355170822	21.848692412208823	22.881035424633357	29.254387807987005
33	26.99126407156831	21.44201183155673	23.502028733780637	28.064695363094323
34	26.881539689876877	21.48963688233344	23.544050837407145	28.08477259038254
35	26.6368776643181	22.283854640874434	23.68085613032455	27.398411564482917
36	26.61446587571729	21.31034257352701	24.490481993528597	27.5847095572271
37	26.92729709160352	20.632385968352686	24.224808916156565	28.21550802388723
38	25.819314292651267	20.5100549555733	25.89775555275408	27.772875199021353
39	25.29310417279489	19.5986422191406	26.780219728910744	28.328033879153768
40	25.02042741148511	20.433014432258034	27.69956997380622	26.846988182450637
41	23.59354353723392	19.809686561798173	28.513398047372917	28.08337185359499
42	22.49863428163214	20.2425142291512	29.195089950647375	28.06376153856929
43	21.899585848823147	20.78273171688308	28.87665578761095	28.441026646682822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	4.0
6	5.0
7	6.0
8	6.0
9	6.0
10	17.5
11	29.0
12	29.0
13	44.5
14	60.0
15	100.0
16	140.0
17	139.0
18	138.0
19	138.0
20	176.0
21	214.0
22	213.0
23	212.0
24	272.0
25	332.0
26	332.0
27	438.5
28	545.0
29	763.0
30	981.0
31	1199.0
32	1417.0
33	1417.0
34	1823.5
35	2230.0
36	2647.5
37	3065.0
38	4205.5
39	5346.0
40	5346.0
41	6233.5
42	7121.0
43	8083.5
44	9046.0
45	11101.0
46	13156.0
47	13156.0
48	15151.5
49	17147.0
50	19488.0
51	21829.0
52	23013.5
53	24198.0
54	24198.0
55	22877.0
56	21556.0
57	20551.0
58	19546.0
59	18272.0
60	16998.0
61	16998.0
62	15936.0
63	14874.0
64	12679.5
65	10485.0
66	9135.0
67	7785.0
68	7785.0
69	6703.0
70	5621.0
71	4741.0
72	3861.0
73	3158.0
74	2455.0
75	2455.0
76	2013.5
77	1572.0
78	1269.0
79	966.0
80	774.0
81	582.0
82	582.0
83	461.0
84	340.0
85	261.0
86	182.0
87	126.5
88	71.0
89	71.0
90	52.0
91	33.0
92	26.0
93	19.0
94	12.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	214173.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.10867145694856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.10147663455292	45.5061916826326
2	7.6532486334196985	8.28214231768094
3	2.3720698595849696	3.8504864611563967
4	1.29830979169066	2.8099927187171696
5	0.8533494398723998	2.3086802240013378
6	0.5562056039516041	1.8057327772038594
7	0.4168278738929483	1.5787801737800347
8	0.3541379497016369	1.5329547176674596
9	0.2765426929898319	1.3467021946925943
>10	1.8910276091401212	20.05405183814256
>50	0.17209244139544214	6.364784631227518
>100	0.05471146980779952	4.55950026309753
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	438	0.2045075709823367	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	333	0.15548178341807792	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	251	0.11719497789170437	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	237	0.11065820621646985	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	226	0.10552217132878561	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	223	0.10412143454123536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.6691226251675046E-4	0.0
8	0.0	0.0	0.0	4.6691226251675046E-4	0.0
9	0.0	0.0	0.0	4.6691226251675046E-4	0.0
10	0.0	0.0	0.0	4.6691226251675046E-4	0.0
11	0.0	0.0	0.0	4.6691226251675046E-4	0.0
12	0.0	0.0	0.0	9.338245250335009E-4	0.0
13	0.0	0.0	0.0	9.338245250335009E-4	0.0
14	0.0	0.0	0.0	9.338245250335009E-4	0.0
15	0.0	0.0	0.0	0.0014007367875502514	0.0
16	0.0	0.0	0.0	0.0014007367875502514	0.0
17	0.0	0.0	0.0	0.0023345613125837525	0.0
18	0.0	0.0	0.0	0.002801473575100503	0.0
19	0.0	0.0	0.0	0.002801473575100503	0.0
20	0.0	0.0	0.0	0.002801473575100503	0.0
21	0.0	0.0	0.0	0.0032683858376172533	0.0
22	0.0	0.0	0.0	0.0037352981001340037	0.0
23	0.0	0.0	0.0	0.005602947150201006	0.0
24	0.0	0.0	0.0	0.008871332987818259	0.0
25	0.0	0.0	0.0	0.01073898203788526	0.0
26	0.0	0.0	0.0	0.014007367875502514	0.0
27	0.0	0.0	0.0	0.035485331951273034	0.0
28	0.0	0.0	0.0	0.12513248635448912	0.0
29	0.0	0.0	0.0	0.2554010075966625	0.0
30	0.0	0.0	0.0	0.44730194749104696	0.0
31	0.0	0.0	0.0	1.0388797840997699	4.6691226251675046E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCTT	20	0.0018385942	37.0	14
GGTATCA	260	0.0	29.884615	1
TTGAGTT	25	0.005486485	29.6	4
CCTTTGA	35	8.845751E-4	26.42857	1
TAGGACA	70	1.9077561E-7	23.785713	4
GTACTGG	65	2.6659563E-6	22.76923	1
TTAGGAC	90	3.785317E-9	22.61111	3
ATCTAAG	50	2.6921657E-4	22.2	32
CATCTAA	50	2.6921657E-4	22.2	31
GTATCAA	355	0.0	21.887325	2
CTTATAC	290	0.0	21.689655	37
GCTTAGG	70	5.0700073E-6	21.142857	1
TAGCGCA	100	1.2751116E-8	20.35	4
TTCTCGT	55	5.124337E-4	20.181818	11
CGGTAAT	185	0.0	20.0	24
TGAAACA	65	6.8686684E-5	19.923077	26
GGTAATA	205	0.0	19.853657	25
ATAGCGC	85	1.235654E-6	19.588236	3
GCGGTAA	190	0.0	19.473684	23
CATAGCG	95	1.6602644E-7	19.473684	2
>>END_MODULE