FastQCFastQC Report
Fri 10 Feb 2017
ERR1632529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151653
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2830.1866102220200062No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2470.16287181921887467No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1940.1279236150949866No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT1920.12660481493936815RNA PCR Primer, Index 41 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1910.12594541486155897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCAA200.001836902837.022
ACCTCTA308.264462E-637.031
TACCAAA251.2285789E-436.99999618
CAAAAAC303.584892E-430.83333221
TTACCAA303.584892E-430.83333217
ACGAATG250.00548149729.59999818
GGTATCA1350.027.4074081
TCTTACC358.833791E-426.4285722
CATTACA358.833791E-426.42857237
GCATTAC358.833791E-426.42857236
ATCTTAC358.833791E-426.4285721
CCAAAAA358.833791E-426.42857220
GCCTGTT400.001923508623.12511
AGCGAAA400.001923508623.12511
ACCCCGC400.001923508623.1256
CCTGTTT502.6871442E-422.19999912
ACCATTG603.698905E-521.5833328
GTAATAC1059.622454E-1021.14285926
CCATTGC705.053902E-621.1428599
ACTTAGA450.00381074220.5555551
Produced by FastQC (version 0.11.5)