##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632529.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151653
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05656333867447	34.0	33.0	34.0	31.0	34.0
2	33.19683092322605	34.0	33.0	34.0	31.0	34.0
3	33.27166623805662	34.0	34.0	34.0	31.0	34.0
4	36.553038845258584	37.0	37.0	37.0	35.0	37.0
5	36.51538710081568	37.0	37.0	37.0	35.0	37.0
6	36.56387938253777	37.0	37.0	37.0	35.0	37.0
7	36.557733773812586	37.0	37.0	37.0	35.0	37.0
8	36.53243918682782	37.0	37.0	37.0	35.0	37.0
9	38.316043863293174	39.0	39.0	39.0	37.0	39.0
10	38.35166465549643	39.0	39.0	39.0	37.0	39.0
11	38.425405366197836	39.0	39.0	39.0	37.0	39.0
12	38.39090555412685	39.0	39.0	39.0	37.0	39.0
13	38.41494728096378	39.0	39.0	39.0	37.0	39.0
14	39.988645130660124	41.0	40.0	41.0	38.0	41.0
15	39.97912998753734	41.0	40.0	41.0	38.0	41.0
16	39.94660837569979	41.0	40.0	41.0	38.0	41.0
17	39.936011816449394	41.0	40.0	41.0	38.0	41.0
18	39.933387404139715	41.0	40.0	41.0	38.0	41.0
19	39.938438408735735	41.0	40.0	41.0	38.0	41.0
20	39.90705096503201	41.0	40.0	41.0	38.0	41.0
21	39.87133126281709	41.0	40.0	41.0	38.0	41.0
22	39.853903318760594	41.0	40.0	41.0	38.0	41.0
23	39.80777828331784	41.0	40.0	41.0	38.0	41.0
24	39.799080796291534	41.0	40.0	41.0	38.0	41.0
25	39.745985902026334	41.0	40.0	41.0	38.0	41.0
26	39.67508720566029	41.0	40.0	41.0	37.0	41.0
27	39.59531957824771	41.0	40.0	41.0	37.0	41.0
28	39.546774544519394	41.0	40.0	41.0	37.0	41.0
29	39.506366507751245	41.0	40.0	41.0	37.0	41.0
30	39.47011928547408	41.0	40.0	41.0	37.0	41.0
31	39.41356913480116	41.0	39.0	41.0	37.0	41.0
32	39.34608613083817	41.0	39.0	41.0	36.0	41.0
33	39.28637085979176	41.0	39.0	41.0	36.0	41.0
34	39.23896658819806	41.0	39.0	41.0	36.0	41.0
35	39.16526544150132	41.0	39.0	41.0	35.0	41.0
36	39.11885686402511	41.0	39.0	41.0	35.0	41.0
37	39.07451220879244	41.0	39.0	41.0	35.0	41.0
38	39.000791280093374	41.0	39.0	41.0	35.0	41.0
39	38.92497345914687	40.0	39.0	41.0	35.0	41.0
40	38.8486940581459	40.0	39.0	41.0	35.0	41.0
41	38.80053807046349	40.0	38.0	41.0	35.0	41.0
42	38.736635608923	40.0	38.0	41.0	35.0	41.0
43	38.00801171094538	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	5.0
20	17.0
21	25.0
22	36.0
23	50.0
24	99.0
25	133.0
26	191.0
27	256.0
28	344.0
29	496.0
30	654.0
31	790.0
32	1164.0
33	1476.0
34	2196.0
35	3536.0
36	5697.0
37	11038.0
38	28970.0
39	94477.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.21378409922652	16.30102932352146	12.603773087245223	27.88141349000679
2	21.20103130172169	19.152275259968484	32.32774821467429	27.318945223635538
3	21.47138533362347	19.913882349838115	28.251336933657754	30.363395382880654
4	16.258168318463863	15.021793172571593	32.581617244630834	36.13842126433371
5	17.411459054552168	32.86779687840003	32.77811846781798	16.94262559922982
6	36.304590083941626	32.38841302183273	14.514714512736312	16.792282381489322
7	31.132915273684002	27.278062418811366	19.491866300040222	22.09715600746441
8	27.918999294441914	30.925863649251912	19.650122318714434	21.505014737591736
9	27.813495281992445	13.157669152604958	17.12791702109421	41.90091854430839
10	19.33558848159944	23.76543820432171	29.838512920944527	27.060460393134328
11	39.41827725135671	19.439773693893294	18.52650458612754	22.61544446862245
12	23.367819957402755	23.499699972964596	26.00542026863959	27.12705980099306
13	34.37254785596065	17.679834886220515	21.0295872814913	26.91802997632754
14	24.567268698937706	19.34745768300001	22.333221235320107	33.75205238274218
15	29.336050061653907	24.1228330464943	20.04444356524434	26.49667332660745
16	27.123762800604013	23.294626548765933	21.72987016412468	27.851740486505378
17	27.165964405583797	23.449585567051095	21.3948949245976	27.9895551027675
18	26.388531713846742	21.54655694249372	23.611797986192162	28.453113357467373
19	28.530922566648865	22.08858380645289	22.932615906048675	26.447877720849572
20	29.30835525838592	21.04475348328091	22.707760479515738	26.939130778817432
21	27.6169940588053	22.358278438276855	21.801744772605883	28.222982730311962
22	28.215729329456064	22.622697869478348	21.08629568818289	28.075277112882702
23	28.13132611949648	22.489499053760888	21.99758659571522	27.381588231027408
24	28.395086150620163	22.073417604663277	21.697559560312026	27.83393668440453
25	28.677309383922506	22.20331941999169	22.26398422715014	26.85538696893566
26	28.545429368360665	22.223101422325968	21.944175189412675	27.287294019900692
27	27.67634006580813	21.659973755876905	23.141645730714195	27.52204044760077
28	26.5929457379676	22.58181506465418	22.383335641233607	28.44190355614462
29	27.40137023336169	23.242533942619005	22.09715600746441	27.2589398165549
30	26.869234370569657	22.557417261775235	23.25374374394176	27.319604623713346
31	27.75942447561209	22.202660019913882	22.432790647069297	27.605124857404732
32	25.857055251132522	21.764818368248566	23.08691552425603	29.29121085636288
33	26.6147059405353	21.783940970505032	23.643449189927004	27.95790389903266
34	26.594923938201028	21.33686771775039	24.026560635134157	28.04164770891443
35	26.345670708789143	22.368828839521804	24.0298576355232	27.255642816165853
36	26.114221281478113	21.4305025287993	24.678707312087464	27.77656887763513
37	26.778896559909793	20.52646502212287	24.543530296136577	28.15110812183076
38	25.593295220008837	20.506023619710785	26.20587789229359	27.694803267986785
39	24.95565534476733	19.62836211614673	27.250367615543382	28.16561492354256
40	24.609470303917497	20.240285388353676	28.364753747040943	26.785490560687887
41	23.109994526979357	19.64352831793634	29.088115632397642	28.158361522686658
42	21.914502185911257	20.524486821889447	29.675641101725653	27.885369890473648
43	21.19575610109922	20.700546642664506	29.73300890849505	28.370688347741225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	7.5
7	12.0
8	9.0
9	6.0
10	11.0
11	16.0
12	16.0
13	26.5
14	37.0
15	66.0
16	95.0
17	102.0
18	109.0
19	109.0
20	144.0
21	179.0
22	175.0
23	171.0
24	213.0
25	255.0
26	255.0
27	353.5
28	452.0
29	679.5
30	907.0
31	1030.5
32	1154.0
33	1154.0
34	1479.5
35	1805.0
36	2018.5
37	2232.0
38	3093.5
39	3955.0
40	3955.0
41	4683.5
42	5412.0
43	6049.5
44	6687.0
45	8155.0
46	9623.0
47	9623.0
48	10932.5
49	12242.0
50	13744.0
51	15246.0
52	16382.5
53	17519.0
54	17519.0
55	16485.0
56	15451.0
57	14592.0
58	13733.0
59	12782.5
60	11832.0
61	11832.0
62	11020.5
63	10209.0
64	8545.0
65	6881.0
66	6039.0
67	5197.0
68	5197.0
69	4417.5
70	3638.0
71	3174.5
72	2711.0
73	2117.0
74	1523.0
75	1523.0
76	1239.5
77	956.0
78	780.0
79	604.0
80	495.0
81	386.0
82	386.0
83	294.0
84	202.0
85	152.0
86	102.0
87	79.5
88	57.0
89	57.0
90	46.5
91	36.0
92	20.5
93	5.0
94	7.0
95	9.0
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	151653.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.50949865812084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.11849143984305	53.58614732316539
2	6.029094885346426	7.416932075197985
3	2.052936825291324	3.788253447013907
4	1.0870380892143094	2.674526715594152
5	0.6903871099152025	2.1232682505456535
6	0.4738371158113657	1.748729006350023
7	0.3687782077807914	1.5878353873645759
8	0.2926641009423141	1.4401297699353128
9	0.2347744985581201	1.2996775533619513
>10	1.5276422851384526	17.873039109018617
>50	0.09219455194519785	3.7922098474807617
>100	0.03216089021344111	2.6692515149716787
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	283	0.1866102220200062	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	247	0.16287181921887467	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	194	0.1279236150949866	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	192	0.12660481493936815	RNA PCR Primer, Index 41 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	191	0.12594541486155897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	6.594000778092091E-4	0.0
18	0.0	0.0	0.0	6.594000778092091E-4	0.0
19	0.0	0.0	0.0	0.0013188001556184183	0.0
20	6.594000778092091E-4	0.0	0.0	0.0013188001556184183	0.0
21	6.594000778092091E-4	0.0	0.0	0.0026376003112368366	0.0
22	6.594000778092091E-4	0.0	0.0	0.006594000778092092	0.0
23	6.594000778092091E-4	0.0	0.0	0.006594000778092092	0.0
24	6.594000778092091E-4	0.0	0.0	0.007253400855901301	0.0
25	6.594000778092091E-4	0.0	0.0	0.007253400855901301	0.0
26	6.594000778092091E-4	0.0	0.0	0.012528601478374974	0.0
27	6.594000778092091E-4	0.0	0.0	0.044179805213217016	0.0
28	6.594000778092091E-4	0.0	0.0	0.1503432177404997	0.0
29	6.594000778092091E-4	0.0	0.0	0.331018839060223	0.0
30	6.594000778092091E-4	0.0	0.0	0.5288388624029857	0.0
31	6.594000778092091E-4	0.0	0.0	1.1585659367107806	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCCAA	20	0.0018369028	37.0	22
ACCTCTA	30	8.264462E-6	37.0	31
TACCAAA	25	1.2285789E-4	36.999996	18
CAAAAAC	30	3.584892E-4	30.833332	21
TTACCAA	30	3.584892E-4	30.833332	17
ACGAATG	25	0.005481497	29.599998	18
GGTATCA	135	0.0	27.407408	1
TCTTACC	35	8.833791E-4	26.428572	2
CATTACA	35	8.833791E-4	26.428572	37
GCATTAC	35	8.833791E-4	26.428572	36
ATCTTAC	35	8.833791E-4	26.428572	1
CCAAAAA	35	8.833791E-4	26.428572	20
GCCTGTT	40	0.0019235086	23.125	11
AGCGAAA	40	0.0019235086	23.125	11
ACCCCGC	40	0.0019235086	23.125	6
CCTGTTT	50	2.6871442E-4	22.199999	12
ACCATTG	60	3.698905E-5	21.583332	8
GTAATAC	105	9.622454E-10	21.142859	26
CCATTGC	70	5.053902E-6	21.142859	9
ACTTAGA	45	0.003810742	20.555555	1
>>END_MODULE