FastQCFastQC Report
Fri 10 Feb 2017
ERR1632528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381956
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10290.2694027584329085No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8250.21599346521588872No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7020.18379080312915624No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6160.16127512069453026No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5610.14687555634680435No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5450.14268659217292046No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5330.1395448690425075No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5250.13745038695556555No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4860.12723978678172357No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4830.12645435599912033No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4700.12305082260783964No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4510.11807642765135251No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4480.11729099686874928No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4420.11572013530354282No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4240.11100755060792342No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4210.11022211982532018No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4210.11022211982532018No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4140.10838944799924598No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4060.10629496591230403No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4000.10472410434709757No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3970.10393867356449434No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3950.10341505304275886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5000.031.4499991
AAACCCG303.595019E-430.83333412
GATCTAG303.595019E-430.83333421
GTCATAG250.00549180829.5999981
TACCAAT250.00549180829.5999986
TAGATAA250.00549180829.5999984
CTCTACT250.00549180829.59999828
ATACGGC905.456968E-1226.72222129
ATCTAGC358.8585116E-426.4285722
TCTAGCC358.8585116E-426.4285723
TGATCTA358.8585116E-426.4285720
GCCGTCT509.070356E-625.89999836
TTCAAAT451.3209539E-424.66666628
CGTATGC551.8983494E-523.54545431
ATGCCGT551.8983494E-523.54545434
GACCCGA400.001928840223.1256
CAGGGGT400.001928840223.1254
ACGGACG400.001928840223.1258
CGTGTCT400.001928840223.12510
CGAGACA652.6750658E-622.7692313
Produced by FastQC (version 0.11.5)