##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632528.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381956
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05123364994921	34.0	33.0	34.0	31.0	34.0
2	33.18442176585785	34.0	33.0	34.0	31.0	34.0
3	33.25882300579124	34.0	34.0	34.0	31.0	34.0
4	36.53254039732325	37.0	37.0	37.0	35.0	37.0
5	36.50985192011645	37.0	37.0	37.0	35.0	37.0
6	36.562541235116086	37.0	37.0	37.0	35.0	37.0
7	36.55954350762915	37.0	37.0	37.0	35.0	37.0
8	36.53758548105017	37.0	37.0	37.0	35.0	37.0
9	38.32897768329337	39.0	39.0	39.0	37.0	39.0
10	38.363829865220076	39.0	39.0	39.0	37.0	39.0
11	38.428664558221364	39.0	39.0	39.0	37.0	39.0
12	38.39346678675031	39.0	39.0	39.0	37.0	39.0
13	38.424889254259654	39.0	39.0	39.0	37.0	39.0
14	39.97715705473929	41.0	40.0	41.0	38.0	41.0
15	39.96788111719675	41.0	40.0	41.0	38.0	41.0
16	39.937272879598694	41.0	40.0	41.0	38.0	41.0
17	39.94187288588214	41.0	40.0	41.0	38.0	41.0
18	39.93981243912911	41.0	40.0	41.0	38.0	41.0
19	39.94700174889254	41.0	40.0	41.0	38.0	41.0
20	39.9165165621171	41.0	40.0	41.0	38.0	41.0
21	39.87988407041649	41.0	40.0	41.0	38.0	41.0
22	39.85711705013143	41.0	40.0	41.0	38.0	41.0
23	39.825773125700344	41.0	40.0	41.0	38.0	41.0
24	39.80722386871786	41.0	40.0	41.0	38.0	41.0
25	39.76178931604687	41.0	40.0	41.0	38.0	41.0
26	39.68236655531003	41.0	40.0	41.0	38.0	41.0
27	39.59407628103761	41.0	40.0	41.0	37.0	41.0
28	39.5554854485857	41.0	40.0	41.0	37.0	41.0
29	39.526353820858944	41.0	40.0	41.0	37.0	41.0
30	39.47711778320016	41.0	40.0	41.0	37.0	41.0
31	39.41355025186147	41.0	39.0	41.0	36.0	41.0
32	39.37107939134351	41.0	39.0	41.0	36.0	41.0
33	39.314030935500426	41.0	39.0	41.0	36.0	41.0
34	39.2757359486433	41.0	39.0	41.0	36.0	41.0
35	39.19242268742997	41.0	39.0	41.0	35.0	41.0
36	39.148014430981576	41.0	39.0	41.0	35.0	41.0
37	39.0916597723298	41.0	39.0	41.0	35.0	41.0
38	39.01700719454597	41.0	39.0	41.0	35.0	41.0
39	38.92578988155704	40.0	39.0	41.0	35.0	41.0
40	38.84652420697672	40.0	39.0	41.0	35.0	41.0
41	38.78310067128151	40.0	38.0	41.0	35.0	41.0
42	38.716420215941106	40.0	38.0	41.0	35.0	41.0
43	38.019263998994646	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	2.0
17	2.0
18	2.0
19	14.0
20	21.0
21	49.0
22	88.0
23	120.0
24	219.0
25	323.0
26	505.0
27	624.0
28	849.0
29	1177.0
30	1610.0
31	2130.0
32	2817.0
33	3788.0
34	5742.0
35	8900.0
36	14180.0
37	27143.0
38	73319.0
39	238331.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.08349652839594	16.601388641623643	12.339117594696772	27.975997235283646
2	21.65458848663197	19.35222905256103	31.982479657342733	27.010702803464277
3	21.5893977316759	19.97036307846977	28.70173527840903	29.738503911445296
4	15.67798385154311	15.64656662023898	34.388777764978165	34.286671763239745
5	17.25146351935825	32.8901758317712	33.01584475698772	16.842515891882833
6	34.01308004063296	34.09319398045848	15.090219815895026	16.80350616301354
7	30.058174239964814	27.403941815287624	19.6454565447329	22.89242740001466
8	26.850736734074083	30.747258846568716	20.188189215511734	22.21381520384547
9	27.770999801024203	12.814565028432595	17.56930117605169	41.84513399449151
10	19.694153253254303	22.647111185581586	29.735362188314884	27.92337337284923
11	39.35374755207406	19.554608384211793	18.822586894825584	22.269057168888562
12	23.040350197404937	23.778393322791107	26.25119123668695	26.930065243117006
13	34.659489574715415	17.924315889788353	20.790876436029283	26.625318099466956
14	24.247819120526973	19.781597880384126	22.747908136015667	33.22267486307323
15	29.71598822901067	23.81242865670391	20.14106336855554	26.330519745729873
16	26.054571730775272	23.64670276157463	22.45075348993078	27.847972017719318
17	26.935039638073494	23.371278367141766	21.669249861240562	28.02443213354418
18	26.277372262773724	21.363193666286172	23.73859816313921	28.620835907800902
19	28.247494475803496	22.06275068332478	23.25582004209909	26.433934798772636
20	29.522772256490278	20.7639099791599	22.919132046623172	26.79418571772665
21	27.544533925373603	22.207269947323777	22.01326854402078	28.234927583281845
22	28.206128454586395	23.079621736535096	21.13437149828776	27.57987831059075
23	28.338080826063734	22.25544303532344	22.305972415670915	27.10050372294191
24	28.278126276325022	21.792824304370136	21.80382033532658	28.125229083978258
25	28.503283100671283	22.274555184366783	22.586633015321137	26.635528699640798
26	28.30535454345527	22.85996292766706	22.005152425933876	26.829530102943792
27	27.590088910764592	21.851469802804512	23.462911958445474	27.095529327985425
28	26.373456628512187	23.026997874100683	22.37718480662694	28.22236069076019
29	27.7620982521547	23.252678318968677	22.14207919236771	26.843144236508916
30	27.068301060855177	22.142341002628573	24.011404454963397	26.777953481552846
31	27.652399752851114	22.3263936160186	22.688477206798687	27.332729424331596
32	25.357632816345337	21.945197876195163	23.138005424708606	29.559163882750894
33	26.188880394600428	21.851207992543646	24.41040329252584	27.54950832033009
34	26.13808920399208	21.55536239776309	24.363539255830517	27.94300914241431
35	26.276848642251988	23.11679879357832	24.117437610614836	26.48891495355486
36	26.198305563991664	21.711663123501136	25.001047241043473	27.088984071463727
37	26.81041795390045	21.116044780027018	24.470357842264555	27.603179423807976
38	25.661856339473655	20.6251505409	26.127093173035636	27.585899946590708
39	25.02879912869545	19.862235440731393	27.246070227984376	27.862895202588778
40	24.894752275131165	20.97204913654976	27.983066112327077	26.150132475992
41	23.400601116358953	19.91643016473102	28.932390118233513	27.75057860067652
42	21.77161767323985	20.924923289593565	29.871241713705242	27.432217323461337
43	21.32366031689514	21.232288535852298	29.115395490580063	28.328655656672495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	6.0
4	11.0
5	11.0
6	14.0
7	17.0
8	21.5
9	26.0
10	40.5
11	55.0
12	55.0
13	94.0
14	133.0
15	224.5
16	316.0
17	333.5
18	351.0
19	351.0
20	416.0
21	481.0
22	437.0
23	393.0
24	486.0
25	579.0
26	579.0
27	701.0
28	823.0
29	1232.5
30	1642.0
31	2103.0
32	2564.0
33	2564.0
34	3396.5
35	4229.0
36	4955.5
37	5682.0
38	7622.0
39	9562.0
40	9562.0
41	11358.5
42	13155.0
43	15502.5
44	17850.0
45	22051.0
46	26252.0
47	26252.0
48	30047.5
49	33843.0
50	37498.5
51	41154.0
52	42787.5
53	44421.0
54	44421.0
55	42188.5
56	39956.0
57	38017.5
58	36079.0
59	32991.0
60	29903.0
61	29903.0
62	27056.5
63	24210.0
64	19947.5
65	15685.0
66	13494.5
67	11304.0
68	11304.0
69	9587.5
70	7871.0
71	6738.0
72	5605.0
73	4369.5
74	3134.0
75	3134.0
76	2479.5
77	1825.0
78	1515.5
79	1206.0
80	947.5
81	689.0
82	689.0
83	558.0
84	427.0
85	341.0
86	255.0
87	201.5
88	148.0
89	148.0
90	108.0
91	68.0
92	52.0
93	36.0
94	22.5
95	9.0
96	9.0
97	6.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	381956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.80345751580544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.88341632738035	36.305802058612194
2	7.482416686929757	6.555114429229627
3	2.6645467928666204	3.5014908672062592
4	1.4769721470078012	2.5878594677393667
5	0.9338186730002598	2.0452243285116345
6	0.6779014077626292	1.7816655308901017
7	0.49348917530822645	1.513157248758663
8	0.39845299765964004	1.3962895164023492
9	0.2771787284468799	1.0927247990227091
>10	2.2666759763080178	19.694588351270074
>50	0.2577074716120292	7.7308972492226475
>100	0.18261788198148457	14.39109943693791
>500	0.0042050170193105	1.1333211181893106
>1k	6.007167170443572E-4	0.2707655980071513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1029	0.2694027584329085	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	825	0.21599346521588872	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	702	0.18379080312915624	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	616	0.16127512069453026	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	561	0.14687555634680435	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	545	0.14268659217292046	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	533	0.1395448690425075	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	525	0.13745038695556555	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	486	0.12723978678172357	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	483	0.12645435599912033	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	470	0.12305082260783964	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	451	0.11807642765135251	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	448	0.11729099686874928	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	442	0.11572013530354282	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	424	0.11100755060792342	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	421	0.11022211982532018	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	421	0.11022211982532018	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	414	0.10838944799924598	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	406	0.10629496591230403	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	400	0.10472410434709757	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	397	0.10393867356449434	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	395	0.10341505304275886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.236205217354879E-4	0.0	0.0	0.0	0.0
9	5.236205217354879E-4	0.0	0.0	0.0	0.0
10	5.236205217354879E-4	0.0	0.0	0.0	0.0
11	5.236205217354879E-4	0.0	0.0	0.0	0.0
12	5.236205217354879E-4	0.0	0.0	0.0	0.0
13	5.236205217354879E-4	0.0	0.0	0.0	2.618102608677439E-4
14	5.236205217354879E-4	0.0	0.0	0.0	2.618102608677439E-4
15	5.236205217354879E-4	0.0	0.0	5.236205217354879E-4	2.618102608677439E-4
16	5.236205217354879E-4	0.0	0.0	7.854307826032318E-4	2.618102608677439E-4
17	5.236205217354879E-4	0.0	0.0	0.0010472410434709757	2.618102608677439E-4
18	5.236205217354879E-4	0.0	0.0	0.0013090513043387197	2.618102608677439E-4
19	5.236205217354879E-4	0.0	0.0	0.0018326718260742074	2.618102608677439E-4
20	5.236205217354879E-4	0.0	0.0	0.0028799128695451834	2.618102608677439E-4
21	5.236205217354879E-4	0.0	0.0	0.004712584695619391	2.618102608677439E-4
22	5.236205217354879E-4	0.0	0.0	0.00863973860863555	2.618102608677439E-4
23	5.236205217354879E-4	0.0	0.0	0.010210600173842014	2.618102608677439E-4
24	5.236205217354879E-4	0.0	0.0	0.01204327199991622	2.618102608677439E-4
25	5.236205217354879E-4	0.0	0.0	0.01256689252165171	2.618102608677439E-4
26	5.236205217354879E-4	0.0	0.0	0.01466137460859366	2.618102608677439E-4
27	5.236205217354879E-4	0.0	0.0	0.041104210956235794	2.618102608677439E-4
28	5.236205217354879E-4	0.0	0.0	0.14085392034684624	2.618102608677439E-4
29	5.236205217354879E-4	0.0	0.0	0.2947983537370797	2.618102608677439E-4
30	5.236205217354879E-4	0.0	0.0	0.4982249264313167	2.618102608677439E-4
31	5.236205217354879E-4	0.0	0.0	1.08389447999246	2.618102608677439E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	500	0.0	31.449999	1
AAACCCG	30	3.595019E-4	30.833334	12
GATCTAG	30	3.595019E-4	30.833334	21
GTCATAG	25	0.005491808	29.599998	1
TACCAAT	25	0.005491808	29.599998	6
TAGATAA	25	0.005491808	29.599998	4
CTCTACT	25	0.005491808	29.599998	28
ATACGGC	90	5.456968E-12	26.722221	29
ATCTAGC	35	8.8585116E-4	26.42857	22
TCTAGCC	35	8.8585116E-4	26.42857	23
TGATCTA	35	8.8585116E-4	26.42857	20
GCCGTCT	50	9.070356E-6	25.899998	36
TTCAAAT	45	1.3209539E-4	24.666666	28
CGTATGC	55	1.8983494E-5	23.545454	31
ATGCCGT	55	1.8983494E-5	23.545454	34
GACCCGA	40	0.0019288402	23.125	6
CAGGGGT	40	0.0019288402	23.125	4
ACGGACG	40	0.0019288402	23.125	8
CGTGTCT	40	0.0019288402	23.125	10
CGAGACA	65	2.6750658E-6	22.76923	13
>>END_MODULE