FastQCFastQC Report
Fri 10 Feb 2017
ERR1632527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168058
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2600.15470849349629295No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2410.14340287281771769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGAC250.005483165429.5999983
GGTATCA1550.028.645161
AAATTTC358.837791E-426.4285727
TAGGACA358.837791E-426.428574
CGTCAAT701.9026811E-723.78571330
GTCCTAA551.8901563E-523.5454541
CTAAGGA551.8901563E-523.5454543
TGCGGGT802.690831E-823.12521
TCCTAAA400.001924370823.1252
CCTCTAG400.001924370823.1251
AGATGGC400.001924370823.1258
GTATCAA1850.023.02
GGTAACG753.701589E-722.225
TTCTGCG753.701589E-722.218
AAATCTC502.6888232E-422.1999999
GAGGGGT603.7019745E-521.5833348
ACGTCAA806.88764E-720.812529
GTAACGT806.88764E-720.812526
GAAATCT555.118022E-420.1818188
GGGAAAT555.118022E-420.1818186
Produced by FastQC (version 0.11.5)