##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632527.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	168058
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.844803579716526	34.0	31.0	34.0	31.0	34.0
2	33.0005712313606	34.0	33.0	34.0	31.0	34.0
3	33.07558699972628	34.0	33.0	34.0	31.0	34.0
4	36.44868438277261	37.0	37.0	37.0	35.0	37.0
5	36.369491485082534	37.0	37.0	37.0	35.0	37.0
6	36.39985005176784	37.0	37.0	37.0	35.0	37.0
7	36.40089730926228	37.0	37.0	37.0	35.0	37.0
8	36.371550298111366	37.0	37.0	37.0	35.0	37.0
9	38.1274678979876	39.0	38.0	39.0	37.0	39.0
10	38.12162467719478	39.0	38.0	39.0	37.0	39.0
11	38.20290613954706	39.0	38.0	39.0	37.0	39.0
12	38.14722298254174	39.0	38.0	39.0	37.0	39.0
13	38.19002368230016	39.0	38.0	39.0	37.0	39.0
14	39.683002296826096	41.0	40.0	41.0	38.0	41.0
15	39.662943745611635	41.0	40.0	41.0	38.0	41.0
16	39.63138916326506	41.0	40.0	41.0	37.0	41.0
17	39.61837579883136	41.0	40.0	41.0	37.0	41.0
18	39.59806733389663	41.0	40.0	41.0	37.0	41.0
19	39.62045246284021	41.0	40.0	41.0	37.0	41.0
20	39.581793190446156	41.0	40.0	41.0	37.0	41.0
21	39.54503207226077	41.0	39.0	41.0	37.0	41.0
22	39.519761034880815	41.0	39.0	41.0	37.0	41.0
23	39.46410762950886	41.0	39.0	41.0	37.0	41.0
24	39.45323638267741	41.0	39.0	41.0	37.0	41.0
25	39.39063894607814	40.0	39.0	41.0	37.0	41.0
26	39.29674874150591	40.0	39.0	41.0	36.0	41.0
27	39.232140094491186	40.0	39.0	41.0	36.0	41.0
28	39.1752847231313	40.0	39.0	41.0	36.0	41.0
29	39.1322162586726	40.0	39.0	41.0	36.0	41.0
30	39.07659855526068	40.0	39.0	41.0	36.0	41.0
31	39.03018005688512	40.0	39.0	41.0	35.0	41.0
32	38.96932011567435	40.0	39.0	41.0	35.0	41.0
33	38.89879089362006	40.0	39.0	41.0	35.0	41.0
34	38.85963179378548	40.0	38.0	41.0	35.0	41.0
35	38.77370312630163	40.0	38.0	41.0	35.0	41.0
36	38.72355377310215	40.0	38.0	41.0	35.0	41.0
37	38.66869176117769	40.0	38.0	41.0	35.0	41.0
38	38.578139689868976	40.0	38.0	41.0	35.0	41.0
39	38.51106760761166	40.0	38.0	41.0	35.0	41.0
40	38.44578062335622	40.0	38.0	41.0	35.0	41.0
41	38.389758297730545	40.0	38.0	41.0	35.0	41.0
42	38.31702150448059	40.0	38.0	41.0	34.0	41.0
43	37.50684882600055	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	9.0
20	17.0
21	25.0
22	65.0
23	92.0
24	147.0
25	193.0
26	269.0
27	332.0
28	467.0
29	714.0
30	885.0
31	1172.0
32	1641.0
33	2219.0
34	3252.0
35	5118.0
36	8101.0
37	16208.0
38	44733.0
39	82393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.556986278546695	16.252127241785573	12.014899617989027	28.1759868616787
2	21.13199014625903	19.12196979614181	32.01394756572136	27.7320924918778
3	21.046305442168777	19.811017624867606	28.352711563864858	30.789965369098766
4	16.409810898618336	14.857965702317058	32.20197788858608	36.53024551047853
5	17.379714146306632	32.725606635804304	33.111782836877744	16.782896381011316
6	36.96045412893168	32.619095788358784	14.206404931630749	16.214045151078793
7	31.651572671339657	27.155505837270468	19.146961168168133	22.045960323221745
8	28.12838424829523	31.196967713527474	19.257637244284712	21.417010793892587
9	27.88204072403575	13.106784562472479	16.807887753037644	42.20328696045413
10	19.08210260743315	23.68527532161516	30.23182472717752	27.000797343774174
11	39.72676099917886	19.199919075557247	18.462673600780683	22.610646324483213
12	23.681110092944103	23.199728665103713	26.05231527210844	27.066845969843744
13	34.38753287555487	17.38804460364874	20.77497054588297	27.449451974913426
14	24.80691189946328	18.7328184317319	22.125694700639066	34.334574968165754
15	29.110188149329396	23.882826167156576	19.77472063216271	27.232265051351316
16	27.31913982077616	23.23364552713944	21.241476157041024	28.205738495043377
17	27.386973544847613	23.14201049637625	21.629437456116342	27.841578502659797
18	26.565233431315377	21.803782027633318	23.38775898796844	28.243225553082862
19	28.77101952897214	21.93825941044163	22.432731556962477	26.85798950362375
20	29.300003570196004	20.923133680039037	22.55590331909222	27.220959430672742
21	27.532756548334504	22.161991693343968	21.541372621356913	28.763879136964622
22	28.587154434778466	22.41428554427638	20.868985707315332	28.129574313629817
23	28.29737352580657	22.407740184936152	21.808542288971662	27.486344000285616
24	28.609765676135616	21.725237715550584	21.196848706994015	28.468147901319785
25	28.52527103737995	21.803782027633318	22.08285234859394	27.588094586392792
26	28.50622999202656	22.19412345737781	21.763914838924656	27.53573171167097
27	28.03972438086851	21.51995144533435	22.49163979102453	27.948684382772615
28	26.830618000928254	22.32027038284402	22.210784372062026	28.63832724416571
29	27.62022635042664	22.730842923276487	21.96682097847172	27.682109747825155
30	27.192397862642657	22.422020968951195	23.014673505575455	27.37090766283069
31	27.917742684073353	22.029894441204824	22.00549810184579	28.046864772876027
32	25.644717895012437	22.072736793249952	22.782015732663723	29.50052957907389
33	26.448607028525867	21.956110390460438	23.58709493151174	28.00818764950196
34	26.935343750371892	21.346201906484666	23.646598198241083	28.07185614490236
35	26.70744623879851	22.291708814813934	23.74358852300991	27.257256423377648
36	26.550952647300335	21.357507527163243	24.3880089016887	27.70353092384772
37	26.65746349474586	20.558973687655453	24.3880089016887	28.395553915909982
38	25.619726522986113	20.392959573480585	26.02613383474753	27.961180068785772
39	25.04194980304419	19.744968998798036	26.812171988242156	28.400909209915625
40	24.715871901367382	20.182913041926	28.03317902152828	27.068036035178334
41	23.312784871889466	19.90919801497102	28.65498815884992	28.12302895428959
42	22.43332658962977	20.39593473681705	29.3458210855776	27.824917587975577
43	21.666924514155827	20.5637339489938	29.308929060205408	28.46041247664497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	3.5
7	6.0
8	6.0
9	6.0
10	6.5
11	7.0
12	7.0
13	15.5
14	24.0
15	40.5
16	57.0
17	67.5
18	78.0
19	78.0
20	97.0
21	116.0
22	126.5
23	137.0
24	196.0
25	255.0
26	255.0
27	338.0
28	421.0
29	637.5
30	854.0
31	1079.5
32	1305.0
33	1305.0
34	1726.5
35	2148.0
36	2501.5
37	2855.0
38	3736.0
39	4617.0
40	4617.0
41	5303.0
42	5989.0
43	6669.0
44	7349.0
45	8693.0
46	10037.0
47	10037.0
48	11506.5
49	12976.0
50	14461.0
51	15946.0
52	17144.5
53	18343.0
54	18343.0
55	17476.5
56	16610.0
57	16096.5
58	15583.0
59	14571.5
60	13560.0
61	13560.0
62	12633.5
63	11707.0
64	10077.0
65	8447.0
66	7319.5
67	6192.0
68	6192.0
69	5310.0
70	4428.0
71	3763.0
72	3098.0
73	2549.0
74	2000.0
75	2000.0
76	1578.5
77	1157.0
78	941.0
79	725.0
80	605.5
81	486.0
82	486.0
83	355.5
84	225.0
85	196.0
86	167.0
87	123.5
88	80.0
89	80.0
90	61.5
91	43.0
92	28.0
93	13.0
94	11.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	168058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.21109971582703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31810690366414	55.19473562484759
2	6.123904609095776	7.741974897915317
3	2.0447644593766734	3.8775543041111415
4	1.0374415717777454	2.6231129057194953
5	0.6695782446820543	2.116238859607288
6	0.4347221769242916	1.6487560124545677
7	0.37076270972903286	1.6405423030914492
8	0.26483048239659956	1.3392180824449622
9	0.20386950153197936	1.159813385131851
>10	1.4200909871557197	16.955006113733443
>50	0.0909417873535999	3.945007942605044
>100	0.020986566312369205	1.7580395683378447
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	260	0.15470849349629295	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	241	0.14340287281771769	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	5.950326672934344E-4	0.0
18	0.0	0.0	0.0	0.0011900653345868688	0.0
19	0.0	0.0	0.0	0.0023801306691737375	0.0
20	0.0	0.0	0.0	0.0035701960037606063	0.0
21	0.0	0.0	0.0	0.004760261338347475	0.0
22	0.0	0.0	0.0	0.0071403920075212126	0.0
23	0.0	0.0	0.0	0.008330457342108081	0.0
24	0.0	0.0	0.0	0.010115555343988385	0.0
25	0.0	0.0	0.0	0.012495686013162123	0.0
26	0.0	0.0	0.0	0.01487581668233586	0.0
27	0.0	0.0	0.0	0.03927215604136667	0.0
28	0.0	0.0	0.0	0.14994823215794548	0.0
29	0.0	0.0	0.0	0.2987063989813041	0.0
30	0.0	0.0	0.0	0.5016125385283652	0.0
31	0.0	0.0	0.0	1.0960501731545063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGAC	25	0.0054831654	29.599998	3
GGTATCA	155	0.0	28.64516	1
AAATTTC	35	8.837791E-4	26.42857	27
TAGGACA	35	8.837791E-4	26.42857	4
CGTCAAT	70	1.9026811E-7	23.785713	30
GTCCTAA	55	1.8901563E-5	23.545454	1
CTAAGGA	55	1.8901563E-5	23.545454	3
TGCGGGT	80	2.690831E-8	23.125	21
TCCTAAA	40	0.0019243708	23.125	2
CCTCTAG	40	0.0019243708	23.125	1
AGATGGC	40	0.0019243708	23.125	8
GTATCAA	185	0.0	23.0	2
GGTAACG	75	3.701589E-7	22.2	25
TTCTGCG	75	3.701589E-7	22.2	18
AAATCTC	50	2.6888232E-4	22.199999	9
GAGGGGT	60	3.7019745E-5	21.583334	8
ACGTCAA	80	6.88764E-7	20.8125	29
GTAACGT	80	6.88764E-7	20.8125	26
GAAATCT	55	5.118022E-4	20.181818	8
GGGAAAT	55	5.118022E-4	20.181818	6
>>END_MODULE