FastQCFastQC Report
Fri 10 Feb 2017
ERR1632526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157085
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6640.4227010853996244No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4840.3081134417671961No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3870.24636343380972084No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3660.23299487538593755No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3390.2158067288410733No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3180.20243817041729No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3140.19989177833656938No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3090.19670878823566856No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3080.1960721902154884No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3070.19543559219530826No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3000.19097940605404717No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2940.18715981793296624No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2740.17442785752936307No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2690.1712448674284623No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT2610.16615208326702105TruSeq Adapter, Index 10 (95% over 21bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2580.16424228920648057No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2560.16296909316612024No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2470.15723971098449885No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2470.15723971098449885No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2460.1566031129643187No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2460.1566031129643187No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2420.15405672088359804No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2330.14832733870197662No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2270.14450775058089568No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2260.14387115256071553No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2180.13877836839927427No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2180.13877836839927427No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2110.13432218225801318No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2110.13432218225801318No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2020.12859280007639176No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2010.1279562020562116No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2010.1279562020562116No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1990.1266830060158513No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1950.12413661393513066No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1910.12159022185441004No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1900.12095362383422988No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1890.12031702581404972No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1870.1190438297736894No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1860.11840723175350924No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1840.11713403571314894No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1780.11331444759206798No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1780.11331444759206798No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1770.11267784957188783No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1720.10949485947098704No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1680.10694846739026642No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1640.10440207530954579No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1630.10376547728936562No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1630.10376547728936562No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1610.10249228124900532No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1600.10185568322882517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCTC200.001837101937.09
TAAGGTA200.001837101937.05
GGCCTTT200.001837101937.025
TACGGCT251.2287786E-436.99999630
TACTATA303.585468E-430.8333342
ATACGGC303.585468E-430.83333429
GATTAGA303.585468E-430.8333342
GAAATCT250.00548208529.5999988
CCGTGTG250.00548208529.59999813
TTACTAT250.00548208529.5999981
AAGGTAG250.00548208529.5999986
GTAGCGA250.00548208529.5999989
TAACCCT250.00548208529.59999835
CCTTTCA250.00548208529.59999827
GGTATCA2550.029.0196081
ATTAGAT358.8352006E-426.428573
ACGGCTG358.8352006E-426.4285731
TAGATAC358.8352006E-426.428575
ATGCCGT601.3251629E-624.66666834
GCCGTCT601.3251629E-624.66666836
Produced by FastQC (version 0.11.5)