FastQCFastQC Report
Fri 10 Feb 2017
ERR1632525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552191
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16010.28993590985727763No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10600.1919625636781476No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9600.17385288785945444No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9320.16878217863022035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9160.16588463049922944No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8460.1532078574261442No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8240.14922372874603174No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7970.14433411627498455No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT7390.13383050430014254No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7350.1331061172673948No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7000.12676773073085218No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6940.1256811501817306No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6480.11735069930513173No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT6290.11390986089958005No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC6260.11336657062501924No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC6140.11119340952677606No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6130.11101231276858914No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5900.10684708733028969No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5900.10684708733028969No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5860.10612270029754196No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5780.10467392623204651No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5620.10177637810105562No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5570.10087089431012096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAAG250.005493900329.63
TCAGTAC250.005493900329.63
ATACGGC1000.027.75000229
GTTAAGA358.8635343E-426.42857228
TCTATAC358.8635343E-426.4285723
GGTATCA7750.024.8258081
TACGGCT1200.024.66666630
TAAGGTA1450.024.2413795
GACAGTT400.001929923423.1257
GCCTTAT903.8180588E-922.6111126
AGTCGGT1155.456968E-1222.5217411
CTAAGGT1650.022.4242424
TATACCC502.6996384E-422.25
CTCTATG1800.021.5833321
GCGGTAA7100.021.36619823
TCTATGG1750.021.1428572
GTCCTAA1850.021.01
GCAGTCG1251.8189894E-1120.7200019
TAGCGAA1700.020.6764710
TCGGGTA1800.020.55555525
Produced by FastQC (version 0.11.5)