##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632525.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	552191
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03938673393807	34.0	33.0	34.0	31.0	34.0
2	33.17525276580024	34.0	33.0	34.0	31.0	34.0
3	33.243850406833864	34.0	34.0	34.0	31.0	34.0
4	36.52882788745199	37.0	37.0	37.0	35.0	37.0
5	36.498367412724946	37.0	37.0	37.0	35.0	37.0
6	36.54933890628424	37.0	37.0	37.0	35.0	37.0
7	36.54967936818963	37.0	37.0	37.0	35.0	37.0
8	36.522273995773205	37.0	37.0	37.0	35.0	37.0
9	38.31735939194952	39.0	39.0	39.0	37.0	39.0
10	38.349361000088734	39.0	39.0	39.0	37.0	39.0
11	38.42125279115378	39.0	39.0	39.0	37.0	39.0
12	38.37711045634572	39.0	39.0	39.0	37.0	39.0
13	38.41247684225205	39.0	39.0	39.0	37.0	39.0
14	39.94748737302854	41.0	40.0	41.0	38.0	41.0
15	39.94675393115788	41.0	40.0	41.0	38.0	41.0
16	39.90672249276066	41.0	40.0	41.0	38.0	41.0
17	39.90871274613313	41.0	40.0	41.0	38.0	41.0
18	39.91055993306664	41.0	40.0	41.0	38.0	41.0
19	39.915981970006754	41.0	40.0	41.0	38.0	41.0
20	39.89231624564689	41.0	40.0	41.0	38.0	41.0
21	39.85798030029464	41.0	40.0	41.0	38.0	41.0
22	39.824390473586135	41.0	40.0	41.0	38.0	41.0
23	39.78484618546843	41.0	40.0	41.0	38.0	41.0
24	39.764911054327214	41.0	40.0	41.0	38.0	41.0
25	39.71792006751287	41.0	40.0	41.0	38.0	41.0
26	39.63695351789507	41.0	40.0	41.0	37.0	41.0
27	39.545952396906145	41.0	40.0	41.0	37.0	41.0
28	39.50407377157541	41.0	40.0	41.0	37.0	41.0
29	39.46647084070548	41.0	40.0	41.0	37.0	41.0
30	39.41509369040785	41.0	39.0	41.0	36.0	41.0
31	39.35030632516647	41.0	39.0	41.0	36.0	41.0
32	39.30018598637066	41.0	39.0	41.0	36.0	41.0
33	39.238868435016144	41.0	39.0	41.0	36.0	41.0
34	39.19260364620213	41.0	39.0	41.0	35.0	41.0
35	39.12512337216651	41.0	39.0	41.0	35.0	41.0
36	39.06371889436807	41.0	39.0	41.0	35.0	41.0
37	39.011889002174975	41.0	39.0	41.0	35.0	41.0
38	38.93789829968254	40.0	39.0	41.0	35.0	41.0
39	38.84629231552126	40.0	39.0	41.0	35.0	41.0
40	38.767826712134024	40.0	38.0	41.0	35.0	41.0
41	38.7068695433283	40.0	38.0	41.0	35.0	41.0
42	38.63468799745016	40.0	38.0	41.0	35.0	41.0
43	37.89324889395155	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	3.0
17	1.0
18	10.0
19	16.0
20	39.0
21	65.0
22	104.0
23	223.0
24	335.0
25	483.0
26	677.0
27	910.0
28	1363.0
29	1868.0
30	2452.0
31	3218.0
32	4283.0
33	5675.0
34	8864.0
35	13262.0
36	21317.0
37	41432.0
38	109137.0
39	336452.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.984583232975545	16.426019257829267	12.535879795215784	28.053517713979403
2	21.67275453602105	19.387856737976534	31.787551771035748	27.15183695496667
3	21.65283389262049	19.494341631790448	28.708544688341536	30.14427978724753
4	16.118516962427858	15.682979258988286	33.672406830245336	34.526096948338534
5	17.824267327790565	32.42664223067743	32.34659746355881	17.402492977973203
6	34.18817039756171	33.39460440318658	15.239654394946674	17.17757080430503
7	29.853800587115693	27.453363057347914	19.504845243765292	23.187991111771108
8	27.285848556024998	30.513354980432496	20.25494801617556	21.945848447366945
9	27.91063237176991	12.793761578873978	17.553889867817478	41.741716181538635
10	19.637951361032684	22.781972179916007	29.328076698099025	28.25199976095228
11	39.64479681849215	19.520238468211183	18.45774378792845	22.377220925368217
12	22.89388997647553	23.514146373265774	26.418213987551407	27.173749662707287
13	35.53390040764881	17.620533474830268	20.47697264171274	26.36859347580819
14	24.191086055368523	20.096307256003808	22.788853856727112	32.92375283190056
15	29.974048834551816	23.9710534941714	19.512451307609144	26.542446363667644
16	25.75123462714894	23.689448035190722	22.301703577204265	28.257613760456074
17	27.022352772863012	23.32707342205867	21.594520736484295	28.056053068594018
18	26.66957628791487	21.035475043961238	23.35206477468847	28.942883893435422
19	28.49376393313183	22.19684855421403	22.909645394437796	26.39974211821634
20	29.92388503253403	20.66712423780902	22.647960578857678	26.76103015079927
21	27.826060185696615	21.897314516172848	21.634724216801796	28.64190108132874
22	28.519117479278005	22.81438849963147	20.940037052396725	27.726456968693803
23	28.475292063796765	21.605748735491886	22.087466112269123	27.831493088442222
24	28.081225517982	21.254239927851053	22.327600413624996	28.336934140541953
25	28.33639085026739	22.244477001617195	22.279247579189086	27.13988456892633
26	28.38945220041616	22.474650981272784	21.87829935656322	27.257597461747835
27	27.401750481264635	21.696840404859913	23.83179008712565	27.069619026749802
28	26.582649843985145	22.928660554047422	21.797168008895472	28.69152159307196
29	27.45390634762247	23.26441394372599	21.94675393115788	27.33492577749366
30	26.840531627643333	22.38048066701558	23.675684681568516	27.103303023772575
31	28.135916738954457	22.04418398706245	22.375409957786346	27.44448931619675
32	25.41113491527388	21.97210747730405	22.794648952989093	29.822108654432977
33	26.406442698269256	21.541640483093712	24.176417217955382	27.875499600681646
34	26.080830727049154	21.441131782299966	24.034618456295014	28.443419034355866
35	26.164678526089702	22.837568884679396	24.26913875814709	26.72861383108381
36	26.107270853744446	21.32396217975302	24.970707599363266	27.59805936713927
37	26.662694611103767	20.778679840852167	24.711014848123202	27.84761069992086
38	25.704873857053084	20.085260353754407	26.251423873261242	27.958441915931264
39	24.92778766767296	19.147903533378848	27.63989271828045	28.28441608066774
40	24.689645430657144	20.28935640023108	28.360295622348065	26.66070254676371
41	23.028082674292047	19.35725138584294	29.586682868789964	28.027983071075045
42	21.41795139725204	20.131983317366636	30.574022394425114	27.87604289095621
43	20.574945987891873	20.97516982348499	29.892193099851323	28.55769108877182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	5.0
2	5.0
3	14.5
4	24.0
5	24.0
6	25.5
7	27.0
8	33.0
9	39.0
10	57.0
11	75.0
12	75.0
13	150.5
14	226.0
15	392.0
16	558.0
17	570.0
18	582.0
19	582.0
20	694.0
21	806.0
22	787.5
23	769.0
24	905.0
25	1041.0
26	1041.0
27	1252.0
28	1463.0
29	2066.5
30	2670.0
31	3140.0
32	3610.0
33	3610.0
34	4572.0
35	5534.0
36	6373.0
37	7212.0
38	9437.5
39	11663.0
40	11663.0
41	13728.0
42	15793.0
43	18722.5
44	21652.0
45	29355.0
46	37058.0
47	37058.0
48	43038.0
49	49018.0
50	54705.5
51	60393.0
52	62948.5
53	65504.0
54	65504.0
55	61916.5
56	58329.0
57	56278.0
58	54227.0
59	50026.5
60	45826.0
61	45826.0
62	41407.5
63	36989.0
64	30213.0
65	23437.0
66	20059.5
67	16682.0
68	16682.0
69	14148.0
70	11614.0
71	9799.5
72	7985.0
73	6289.0
74	4593.0
75	4593.0
76	3624.5
77	2656.0
78	2182.5
79	1709.0
80	1360.0
81	1011.0
82	1011.0
83	837.0
84	663.0
85	518.0
86	373.0
87	292.0
88	211.0
89	211.0
90	159.0
91	107.0
92	71.5
93	36.0
94	25.0
95	14.0
96	14.0
97	7.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	552191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.54110952083787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.03562872031874	40.902292476868176
2	6.497098577425989	6.177585500741777
3	2.0746166708393576	2.958887350863898
4	1.1382189438412011	2.1644876587138784
5	0.7197004979079061	1.7107680096620657
6	0.4777812879398732	1.362855152217389
7	0.3862185707382294	1.2852881559313343
8	0.2619107381558625	0.9961221669881081
9	0.22519390593855418	0.9635371331085059
>10	1.825696741029811	17.621388227088744
>50	0.19868282836068915	6.559142577632116
>100	0.14853359805642088	13.66955160381645
>500	0.009953282343986966	3.1438021329577723
>1k	7.656371033836128E-4	0.48429185340978537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1601	0.28993590985727763	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1060	0.1919625636781476	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	960	0.17385288785945444	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	932	0.16878217863022035	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	916	0.16588463049922944	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	846	0.1532078574261442	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	824	0.14922372874603174	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	797	0.14433411627498455	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	739	0.13383050430014254	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	735	0.1331061172673948	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	700	0.12676773073085218	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	694	0.1256811501817306	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	648	0.11735069930513173	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	629	0.11390986089958005	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	626	0.11336657062501924	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	614	0.11119340952677606	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	613	0.11101231276858914	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	590	0.10684708733028969	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	590	0.10684708733028969	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	586	0.10612270029754196	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	578	0.10467392623204651	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	562	0.10177637810105562	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	557	0.10087089431012096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.810967581869317E-4
2	0.0	0.0	0.0	0.0	1.810967581869317E-4
3	0.0	0.0	0.0	0.0	1.810967581869317E-4
4	0.0	0.0	0.0	0.0	1.810967581869317E-4
5	0.0	0.0	0.0	0.0	1.810967581869317E-4
6	0.0	0.0	0.0	0.0	1.810967581869317E-4
7	0.0	0.0	0.0	0.0	1.810967581869317E-4
8	0.0	0.0	0.0	0.0	1.810967581869317E-4
9	0.0	0.0	0.0	0.0	1.810967581869317E-4
10	0.0	0.0	0.0	0.0	1.810967581869317E-4
11	0.0	0.0	0.0	0.0	1.810967581869317E-4
12	0.0	0.0	0.0	0.0	1.810967581869317E-4
13	0.0	0.0	0.0	1.810967581869317E-4	1.810967581869317E-4
14	0.0	0.0	0.0	1.810967581869317E-4	1.810967581869317E-4
15	0.0	0.0	0.0	5.432902745607951E-4	1.810967581869317E-4
16	0.0	0.0	0.0	0.0014487740654954536	1.810967581869317E-4
17	0.0	0.0	0.0	0.001810967581869317	1.810967581869317E-4
18	0.0	0.0	0.0	0.0021731610982431804	1.810967581869317E-4
19	0.0	0.0	0.0	0.0025353546146170435	1.810967581869317E-4
20	0.0	0.0	0.0	0.0039841286801124975	1.810967581869317E-4
21	0.0	0.0	0.0	0.005795096261981814	1.810967581869317E-4
22	0.0	0.0	0.0	0.008330450876598857	1.810967581869317E-4
23	0.0	0.0	0.0	0.009054837909346585	1.810967581869317E-4
24	0.0	0.0	0.0	0.011952386040337493	1.810967581869317E-4
25	0.0	0.0	0.0	0.014668837413141468	1.810967581869317E-4
26	0.0	0.0	0.0	0.020645030433310212	1.810967581869317E-4
27	0.0	0.0	0.0	0.0519747695996494	1.810967581869317E-4
28	0.0	0.0	0.0	0.19015159609627827	1.810967581869317E-4
29	0.0	0.0	0.0	0.39044461065102476	1.810967581869317E-4
30	0.0	0.0	0.0	0.6517672327147672	1.810967581869317E-4
31	0.0	0.0	0.0	1.3555092350291837	1.810967581869317E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAAAG	25	0.0054939003	29.6	3
TCAGTAC	25	0.0054939003	29.6	3
ATACGGC	100	0.0	27.750002	29
GTTAAGA	35	8.8635343E-4	26.428572	28
TCTATAC	35	8.8635343E-4	26.428572	3
GGTATCA	775	0.0	24.825808	1
TACGGCT	120	0.0	24.666666	30
TAAGGTA	145	0.0	24.241379	5
GACAGTT	40	0.0019299234	23.125	7
GCCTTAT	90	3.8180588E-9	22.61111	26
AGTCGGT	115	5.456968E-12	22.52174	11
CTAAGGT	165	0.0	22.424242	4
TATACCC	50	2.6996384E-4	22.2	5
CTCTATG	180	0.0	21.583332	1
GCGGTAA	710	0.0	21.366198	23
TCTATGG	175	0.0	21.142857	2
GTCCTAA	185	0.0	21.0	1
GCAGTCG	125	1.8189894E-11	20.720001	9
TAGCGAA	170	0.0	20.67647	10
TCGGGTA	180	0.0	20.555555	25
>>END_MODULE