FastQCFastQC Report
Fri 10 Feb 2017
ERR1632524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6128
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA410.6690600522193211No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT350.5711488250652741RNA PCR Primer, Index 39 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA310.5058746736292429No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA220.35900783289817234No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA210.3426892950391645No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC180.29373368146214096No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA170.27741514360313313No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA170.27741514360313313No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC160.26109660574412535No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA160.26109660574412535No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC160.26109660574412535No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA160.26109660574412535No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC150.2447780678851175No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC150.2447780678851175No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT150.2447780678851175No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA150.2447780678851175No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT140.22845953002610966No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC140.22845953002610966No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA140.22845953002610966No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC130.21214099216710186RNA PCR Primer, Index 39 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT130.21214099216710186No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT120.19582245430809397No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT120.19582245430809397No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT120.19582245430809397No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT120.19582245430809397No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT120.19582245430809397No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA120.19582245430809397No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA120.19582245430809397No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC110.17950391644908617No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG110.17950391644908617No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC110.17950391644908617No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA110.17950391644908617No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC110.17950391644908617No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA110.17950391644908617No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA110.17950391644908617No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT110.17950391644908617No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG110.17950391644908617No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA100.1631853785900783No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA100.1631853785900783No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG100.1631853785900783No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG100.1631853785900783No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT100.1631853785900783No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC100.1631853785900783No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT100.1631853785900783No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC100.1631853785900783No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC100.1631853785900783No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT100.1631853785900783No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC100.1631853785900783No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC90.14686684073107048No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT90.14686684073107048No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT90.14686684073107048No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG90.14686684073107048No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT80.13054830287206268No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA80.13054830287206268No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC80.13054830287206268No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC80.13054830287206268No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGG80.13054830287206268No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA80.13054830287206268No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC80.13054830287206268No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC80.13054830287206268No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT80.13054830287206268No Hit
TCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCG80.13054830287206268No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC80.13054830287206268No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC80.13054830287206268No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA80.13054830287206268No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG80.13054830287206268No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCC80.13054830287206268No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG80.13054830287206268No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA70.11422976501305483No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC70.11422976501305483No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC70.11422976501305483No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAA70.11422976501305483No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG70.11422976501305483No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC70.11422976501305483No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC70.11422976501305483No Hit
TCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCA70.11422976501305483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.11422976501305483No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA70.11422976501305483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.11422976501305483No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT70.11422976501305483No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC70.11422976501305483No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC70.11422976501305483No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC70.11422976501305483No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT70.11422976501305483No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG70.11422976501305483No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC70.11422976501305483No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG70.11422976501305483No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG70.11422976501305483No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers