##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632524.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6128
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97568537859008	34.0	33.0	34.0	31.0	34.0
2	33.087956919060055	34.0	33.0	34.0	31.0	34.0
3	33.14719321148825	34.0	33.0	34.0	31.0	34.0
4	36.47340078328982	37.0	37.0	37.0	35.0	37.0
5	36.44370104438642	37.0	37.0	37.0	35.0	37.0
6	36.492493472584854	37.0	37.0	37.0	35.0	37.0
7	36.51289164490861	37.0	37.0	37.0	35.0	37.0
8	36.46817885117493	37.0	37.0	37.0	35.0	37.0
9	38.2253590078329	39.0	39.0	39.0	37.0	39.0
10	38.25913838120105	39.0	39.0	39.0	37.0	39.0
11	38.326860313315926	39.0	39.0	39.0	37.0	39.0
12	38.29846605744125	39.0	39.0	39.0	37.0	39.0
13	38.359986945169716	39.0	39.0	39.0	37.0	39.0
14	39.87238903394256	41.0	40.0	41.0	38.0	41.0
15	39.860150130548305	41.0	40.0	41.0	38.0	41.0
16	39.82588120104439	41.0	40.0	41.0	38.0	41.0
17	39.849543080939945	41.0	40.0	41.0	38.0	41.0
18	39.83240861618799	41.0	40.0	41.0	38.0	41.0
19	39.82865535248042	41.0	40.0	41.0	38.0	41.0
20	39.81201044386423	41.0	40.0	41.0	38.0	41.0
21	39.7746409921671	41.0	40.0	41.0	38.0	41.0
22	39.73596605744125	41.0	40.0	41.0	38.0	41.0
23	39.68652088772846	41.0	40.0	41.0	37.0	41.0
24	39.677219321148826	41.0	40.0	41.0	37.0	41.0
25	39.66090078328982	41.0	40.0	41.0	37.0	41.0
26	39.567558746736296	41.0	40.0	41.0	37.0	41.0
27	39.34611618798956	41.0	39.0	41.0	36.0	41.0
28	39.36161879895561	41.0	39.0	41.0	36.0	41.0
29	39.347584856396864	41.0	39.0	41.0	36.0	41.0
30	39.34807441253264	41.0	39.0	41.0	36.0	41.0
31	39.346932114882506	41.0	39.0	41.0	36.0	41.0
32	39.28753263707572	41.0	39.0	41.0	36.0	41.0
33	39.139686684073105	41.0	39.0	41.0	35.0	41.0
34	39.18701044386423	41.0	39.0	41.0	35.0	41.0
35	39.130058746736296	40.0	39.0	41.0	35.0	41.0
36	39.00930156657964	40.0	39.0	41.0	35.0	41.0
37	38.92150783289817	40.0	39.0	41.0	35.0	41.0
38	38.80107702349869	40.0	38.0	41.0	35.0	41.0
39	38.67819843342036	40.0	38.0	41.0	35.0	41.0
40	38.55172976501306	40.0	38.0	41.0	35.0	41.0
41	38.525620104438644	40.0	38.0	41.0	35.0	41.0
42	38.41302219321149	40.0	37.0	41.0	35.0	41.0
43	37.713120104438644	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	1.0
23	2.0
24	3.0
25	8.0
26	8.0
27	6.0
28	15.0
29	24.0
30	23.0
31	47.0
32	56.0
33	81.0
34	93.0
35	194.0
36	256.0
37	464.0
38	1294.0
39	3552.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.16710182767624	15.486292428198434	13.430156657963446	28.916449086161876
2	22.552219321148826	19.74543080939948	30.531984334203656	27.17036553524804
3	22.291122715404697	18.472584856396868	29.3733681462141	29.86292428198433
4	15.094647519582246	16.77545691906005	35.003263707571804	33.126631853785895
5	18.390992167101828	32.40861618798956	31.804830287206265	17.39556135770235
6	31.347911227154047	34.61161879895561	16.073759791122715	17.966710182767624
7	28.459530026109658	27.431462140992167	19.74543080939948	24.363577023498696
8	26.109660574412537	30.140339425587463	22.176892950391643	21.573107049608357
9	27.039817232375977	12.58159268929504	17.118146214099216	43.260443864229764
10	20.33289817232376	21.2467362924282	28.639033942558743	29.7813315926893
11	39.49086161879896	19.19060052219321	18.619451697127936	22.699086161879894
12	22.095300261096607	22.486945169712794	27.137728459530024	28.28002610966057
13	37.255221932114885	16.987597911227155	18.864229765013054	26.89295039164491
14	24.592036553524803	19.402741514360315	22.405352480417754	33.59986945169713
15	31.31527415143603	22.405352480417754	18.896866840731068	27.382506527415146
16	25.016318537859007	23.92297650130548	22.97650130548303	28.08420365535248
17	26.990861618798956	23.515013054830288	19.990208877284594	29.50391644908616
18	25.636422976501304	19.48433420365535	24.02088772845953	30.85835509138381
19	27.26827676240209	21.654699738903393	23.596605744125327	27.480417754569192
20	30.221932114882506	19.81070496083551	22.176892950391643	27.790469973890342
21	27.007180156657963	20.92036553524804	21.573107049608357	30.49934725848564
22	28.736945169712797	21.458877284595303	20.463446475195823	29.340731070496084
23	29.3733681462141	21.32832898172324	21.197780678851174	28.100522193211486
24	28.198433420365536	20.283942558746737	22.66644908616188	28.851174934725847
25	29.38968668407311	22.617493472584858	22.06266318537859	25.930156657963447
26	29.52023498694517	21.81788511749347	21.116187989556135	27.54569190600522
27	27.023498694516974	22.470626631853786	24.640992167101828	25.864882506527415
28	26.142297650130548	23.825065274151434	21.785248041775457	28.24738903394256
29	26.41971279373368	23.71083550913838	22.11161879895561	27.757832898172325
30	26.35443864229765	21.54046997389034	24.428851174934728	27.676240208877285
31	28.508485639686683	21.89947780678851	21.230417754569192	28.36161879895561
32	26.403394255874673	21.344647519582246	21.964751958224543	30.287206266318538
33	26.41971279373368	21.54046997389034	23.515013054830288	28.524804177545693
34	25.718015665796344	22.68276762402089	22.470626631853786	29.12859007832898
35	26.028067885117494	24.70626631853786	23.20496083550914	26.06070496083551
36	26.58289817232376	21.85052219321149	24.461488250652742	27.10509138381201
37	29.50391644908616	21.279373368146214	23.237597911227155	25.979112271540473
38	27.96997389033943	20.202349869451698	24.330939947780678	27.4967362924282
39	26.795039164490863	19.288511749347258	25.45691906005222	28.459530026109658
40	27.056135770234985	21.06723237597911	26.827676240208874	25.04895561357702
41	24.23302872062663	20.137075718015666	26.86031331592689	28.769582245430808
42	21.752610966057443	21.752610966057443	29.650783289817234	26.843994778067888
43	21.181462140992167	21.83420365535248	28.62271540469974	28.36161879895561
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	1.0
11	2.0
12	2.0
13	2.5
14	3.0
15	7.0
16	11.0
17	10.5
18	10.0
19	10.0
20	10.0
21	10.0
22	9.0
23	8.0
24	7.5
25	7.0
26	7.0
27	6.5
28	6.0
29	7.5
30	9.0
31	7.5
32	6.0
33	6.0
34	11.0
35	16.0
36	18.5
37	21.0
38	40.5
39	60.0
40	60.0
41	94.0
42	128.0
43	162.0
44	196.0
45	318.5
46	441.0
47	441.0
48	515.0
49	589.0
50	682.0
51	775.0
52	760.0
53	745.0
54	745.0
55	758.5
56	772.0
57	727.5
58	683.0
59	610.0
60	537.0
61	537.0
62	489.5
63	442.0
64	336.0
65	230.0
66	195.0
67	160.0
68	160.0
69	134.0
70	108.0
71	89.5
72	71.0
73	56.5
74	42.0
75	42.0
76	31.5
77	21.0
78	15.5
79	10.0
80	6.5
81	3.0
82	3.0
83	3.5
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6128.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.43276762402088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.24023581429624	54.63446475195822
2	8.867600098255956	11.781984334203656
3	3.217882584131663	6.413185378590078
4	1.7194792434291328	4.569190600522193
5	1.0808155244411692	3.5900783289817233
6	0.7123556865634979	2.839425587467363
7	0.49127978383689513	2.2845953002610964
8	0.39302382706951605	2.088772845953003
9	0.09825595676737901	0.5874673629242819
>10	1.1790714812085483	11.21083550913838
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	41	0.6690600522193211	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	35	0.5711488250652741	RNA PCR Primer, Index 39 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	31	0.5058746736292429	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	22	0.35900783289817234	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	21	0.3426892950391645	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	18	0.29373368146214096	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	17	0.27741514360313313	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	17	0.27741514360313313	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	16	0.26109660574412535	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	16	0.26109660574412535	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	16	0.26109660574412535	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	16	0.26109660574412535	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	15	0.2447780678851175	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	15	0.2447780678851175	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	15	0.2447780678851175	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	15	0.2447780678851175	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	14	0.22845953002610966	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	14	0.22845953002610966	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	14	0.22845953002610966	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	13	0.21214099216710186	RNA PCR Primer, Index 39 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	13	0.21214099216710186	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	12	0.19582245430809397	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	12	0.19582245430809397	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	12	0.19582245430809397	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	12	0.19582245430809397	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	12	0.19582245430809397	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	12	0.19582245430809397	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	12	0.19582245430809397	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	11	0.17950391644908617	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	11	0.17950391644908617	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	11	0.17950391644908617	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	11	0.17950391644908617	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	11	0.17950391644908617	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	11	0.17950391644908617	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	11	0.17950391644908617	No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT	11	0.17950391644908617	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	11	0.17950391644908617	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	10	0.1631853785900783	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	10	0.1631853785900783	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	10	0.1631853785900783	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	10	0.1631853785900783	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	10	0.1631853785900783	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	10	0.1631853785900783	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	10	0.1631853785900783	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	10	0.1631853785900783	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	10	0.1631853785900783	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	10	0.1631853785900783	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	10	0.1631853785900783	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	9	0.14686684073107048	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	9	0.14686684073107048	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	9	0.14686684073107048	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	9	0.14686684073107048	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	8	0.13054830287206268	No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA	8	0.13054830287206268	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	8	0.13054830287206268	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	8	0.13054830287206268	No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGG	8	0.13054830287206268	No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA	8	0.13054830287206268	No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC	8	0.13054830287206268	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	8	0.13054830287206268	No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT	8	0.13054830287206268	No Hit
TCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCG	8	0.13054830287206268	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	8	0.13054830287206268	No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC	8	0.13054830287206268	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	8	0.13054830287206268	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	8	0.13054830287206268	No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCC	8	0.13054830287206268	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	8	0.13054830287206268	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	7	0.11422976501305483	No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC	7	0.11422976501305483	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC	7	0.11422976501305483	No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAA	7	0.11422976501305483	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG	7	0.11422976501305483	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	7	0.11422976501305483	No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC	7	0.11422976501305483	No Hit
TCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCA	7	0.11422976501305483	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.11422976501305483	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	7	0.11422976501305483	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.11422976501305483	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	7	0.11422976501305483	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	7	0.11422976501305483	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	7	0.11422976501305483	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	7	0.11422976501305483	No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT	7	0.11422976501305483	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	7	0.11422976501305483	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	7	0.11422976501305483	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	7	0.11422976501305483	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	7	0.11422976501305483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.03263707571801567	0.0
25	0.0	0.0	0.0	0.03263707571801567	0.0
26	0.0	0.0	0.0	0.03263707571801567	0.0
27	0.0	0.0	0.0	0.03263707571801567	0.0
28	0.0	0.0	0.0	0.13054830287206268	0.0
29	0.0	0.0	0.0	0.2774151436031332	0.0
30	0.0	0.0	0.0	0.391644908616188	0.0
31	0.0	0.0	0.0	0.7180156657963447	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE