FastQCFastQC Report
Fri 10 Feb 2017
ERR1632523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332995
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11820.3549602846889593No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9080.2726767669184222No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6860.20600909923572425No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6150.1846874577696362No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5970.17928197120076877No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5900.1771798375350981No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5400.16216459706602201No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5060.15195423354705026No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4960.14895118545323502No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4710.14144356521869697No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4630.1390411267436448No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4590.13783990750611871No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4440.13333533536539588No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4300.12913106803405455No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4290.12883076322467304No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4280.1285304584152915No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4260.1279298487965285No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4210.12642832474962085No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4180.1255274103214763No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4070.12222405741827956No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3990.11982161894322736No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3920.11771948527755673No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3900.11711887565879367No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3800.11411582756497844No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3720.11171338908992627No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3700.11111277947116324No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3610.10841003618672954No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3530.10600759771167735No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3520.10570729290229582No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3510.10540698809291431No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3470.10420576885538822No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3450.10360515923662518No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3410.10240393999909908No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3390.10180333038033605No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3380.10150302557095452No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3340.10030180633342843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC1050.029.95238329
TTAAACC509.065789E-625.9000024
GGTATCA4500.025.488891
GCCGTCT451.320456E-424.66666636
TACGGCT1350.023.29629730
TAAACCG400.001928322623.1250025
TACACAG400.001928322623.1250025
ACACGAA502.69652E-422.217
CTACCGT502.69652E-422.28
GCTACCG603.716063E-521.5833327
TACACTG603.716063E-521.5833325
CGCGGTA4800.021.19791622
GCGGTAA4800.021.19791623
CACTGTG705.0839844E-621.1428577
GGTAATA4900.020.76530525
GTGCAAA450.003820187620.55555511
AACAACG450.003820187620.55555510
GCTTAAA450.003820187620.5555552
GGACAGC450.003820187620.5555556
GCGTAGA450.003820187620.5555551
Produced by FastQC (version 0.11.5)