##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632523.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332995
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59804501569093	34.0	31.0	34.0	31.0	34.0
2	32.76659709605249	34.0	31.0	34.0	31.0	34.0
3	32.84944818991276	34.0	31.0	34.0	31.0	34.0
4	36.31691767143651	37.0	37.0	37.0	35.0	37.0
5	36.17111368038559	37.0	35.0	37.0	35.0	37.0
6	36.18718599378369	37.0	36.0	37.0	35.0	37.0
7	36.201591615489725	37.0	36.0	37.0	35.0	37.0
8	36.15968708238862	37.0	36.0	37.0	35.0	37.0
9	37.87022327662577	39.0	38.0	39.0	35.0	39.0
10	37.848027748164384	39.0	38.0	39.0	35.0	39.0
11	37.929920869682725	39.0	38.0	39.0	35.0	39.0
12	37.87774891514888	39.0	38.0	39.0	35.0	39.0
13	37.926236129671615	39.0	38.0	39.0	35.0	39.0
14	39.29388128950885	40.0	39.0	41.0	36.0	41.0
15	39.28638568146669	40.0	39.0	41.0	36.0	41.0
16	39.24079941140258	40.0	39.0	41.0	36.0	41.0
17	39.2362227661076	40.0	39.0	41.0	36.0	41.0
18	39.21622546885089	40.0	39.0	41.0	36.0	41.0
19	39.22133965975465	40.0	39.0	41.0	36.0	41.0
20	39.199768765296774	40.0	39.0	41.0	36.0	41.0
21	39.14815537770838	40.0	39.0	41.0	36.0	41.0
22	39.10729890839202	40.0	39.0	41.0	36.0	41.0
23	39.07460472379465	40.0	39.0	41.0	36.0	41.0
24	39.05040916530278	40.0	38.0	41.0	36.0	41.0
25	38.996939893992405	40.0	38.0	41.0	36.0	41.0
26	38.88415441673298	40.0	38.0	41.0	35.0	41.0
27	38.75871709785432	40.0	38.0	41.0	35.0	41.0
28	38.714398114085796	40.0	38.0	41.0	35.0	41.0
29	38.66237030586045	40.0	38.0	41.0	35.0	41.0
30	38.61369990540398	40.0	38.0	41.0	35.0	41.0
31	38.55509542185318	40.0	38.0	41.0	35.0	41.0
32	38.48753284583852	40.0	38.0	41.0	35.0	41.0
33	38.41445967657172	40.0	38.0	41.0	34.0	41.0
34	38.36276820973288	40.0	38.0	41.0	34.0	41.0
35	38.263051997777744	40.0	38.0	41.0	34.0	41.0
36	38.214114326040935	40.0	38.0	41.0	34.0	41.0
37	38.14969894442859	40.0	38.0	41.0	34.0	41.0
38	38.06424420787099	40.0	37.0	41.0	34.0	41.0
39	37.95725461343263	40.0	37.0	41.0	33.0	41.0
40	37.857946816018256	40.0	37.0	41.0	33.0	41.0
41	37.80344449616361	40.0	37.0	41.0	33.0	41.0
42	37.71750927191099	40.0	37.0	41.0	33.0	41.0
43	36.913785492274656	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	1.0
16	1.0
17	4.0
18	6.0
19	11.0
20	28.0
21	61.0
22	103.0
23	175.0
24	316.0
25	422.0
26	618.0
27	887.0
28	1235.0
29	1710.0
30	2322.0
31	3037.0
32	4229.0
33	5894.0
34	8699.0
35	13901.0
36	23266.0
37	46773.0
38	105155.0
39	114139.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.0976441087704	16.403249298037508	12.422108440066667	28.07699815312542
2	22.210243397048004	19.40209312452139	31.360530938902986	27.02713253952762
3	22.254988813645852	19.416507755371704	28.39381972702293	29.934683703959518
4	16.27141548671902	15.782519257045902	33.72813405606691	34.217931200168174
5	17.925194071983064	32.34943467619634	32.113695400831844	17.611675850988753
6	34.06537635700236	33.41761888316641	15.381011726902807	17.135993032928422
7	30.06891995375306	26.969474016126366	19.346837039595187	23.614768990525384
8	27.254763585038816	30.026877280439646	20.26667067073079	22.45168846379075
9	28.00702713253953	12.74433550053304	17.06211804982057	42.186519317106864
10	20.115016741993124	22.358593972882478	28.734665685670958	28.791723599453444
11	39.902400936951004	19.23602456493341	18.44712383068815	22.414450667427438
12	23.02647186894698	23.06551149416658	26.470968032553046	27.4370486043334
13	35.87831649123861	17.144401567591107	20.073874983107853	26.90340695806243
14	24.314178891574947	19.743539692788183	22.63757714079791	33.30470427483896
15	30.29174612231415	23.575729365305786	19.189777624288652	26.942746888091413
16	25.62440877490653	23.622877220378687	22.01024039399991	28.742473610714875
17	27.51572846439136	23.133980990705567	21.439661256175018	27.91062928872806
18	26.6601600624634	20.687097403864925	23.11325995885824	29.539482574813437
19	28.511539212300484	22.23156503851409	22.6198591570444	26.637036592141023
20	29.762308743374525	20.59850748509737	22.233667172179764	27.40551659934834
21	28.54186999804802	21.35737773840448	21.35347377588252	28.74727848766498
22	28.73977086743044	22.59853751557831	20.82313548251475	27.838556134476494
23	29.262601540563672	21.387408219342632	21.412633823330683	27.937356416763016
24	28.62565503986546	20.95647081788015	21.776002642682325	28.641871499572062
25	28.941575699334827	21.855283112359043	22.02645685370651	27.17668433459962
26	29.055991831709182	22.12675866003994	21.637862430366823	27.179387077884055
27	27.888707037643208	21.415036261805735	23.624979354044353	27.071277346506704
28	26.91511884562831	22.788630459916813	21.601225243622277	28.695025450832595
29	27.74846469166204	23.12707398008979	21.64416883136383	27.48029249688434
30	27.399210198351327	21.478400576585237	23.664319284073336	27.458069940990104
31	28.476403549602846	21.612937131188158	22.135467499512004	27.775191819696992
32	26.035225754140452	21.69251790567426	22.500638147719936	29.771618192465354
33	26.984188951786063	21.662487424736106	23.55230559017403	27.801018033303805
34	26.78388564392859	21.75978618297572	23.1480953167465	28.308232856349196
35	26.69769816363609	23.13308007627742	23.51927206114206	26.649949698944432
36	26.903707262871812	21.42584723494347	23.943302451988767	27.727143050195952
37	27.106413009204346	21.07509121758585	23.88624453820628	27.93225123500353
38	26.46285980269974	20.48168891424796	25.404585654439256	27.65086562861304
39	25.58146518716497	19.500292797189147	26.604303367918437	28.313938647727444
40	25.488971305875463	20.38018588867701	27.595909848496227	26.53493295695131
41	23.7559122509347	19.675670805867956	28.233156654003817	28.33526028919353
42	21.80453159957357	20.70421477799967	29.488430757218577	28.002822865208188
43	21.14596315259989	21.220438745326504	28.82085316596345	28.812744936110153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.5
4	4.0
5	4.0
6	8.0
7	12.0
8	17.5
9	23.0
10	31.5
11	40.0
12	40.0
13	77.0
14	114.0
15	195.0
16	276.0
17	264.0
18	252.0
19	252.0
20	323.5
21	395.0
22	372.0
23	349.0
24	419.5
25	490.0
26	490.0
27	598.5
28	707.0
29	915.5
30	1124.0
31	1476.5
32	1829.0
33	1829.0
34	2399.5
35	2970.0
36	3315.5
37	3661.0
38	4912.5
39	6164.0
40	6164.0
41	7477.0
42	8790.0
43	10902.0
44	13014.0
45	17656.5
46	22299.0
47	22299.0
48	25645.0
49	28991.0
50	32483.0
51	35975.0
52	37701.0
53	39427.0
54	39427.0
55	37110.5
56	34794.0
57	33809.5
58	32825.0
59	30417.0
60	28009.0
61	28009.0
62	25782.5
63	23556.0
64	18831.5
65	14107.0
66	12193.0
67	10279.0
68	10279.0
69	8796.5
70	7314.0
71	6373.0
72	5432.0
73	4750.0
74	4068.0
75	4068.0
76	3724.5
77	3381.0
78	2332.5
79	1284.0
80	891.5
81	499.0
82	499.0
83	381.5
84	264.0
85	218.5
86	173.0
87	119.0
88	65.0
89	65.0
90	49.0
91	33.0
92	19.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	332995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.10298150172406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.87253923679607	39.12877106438359
2	6.650793836881394	6.132428504670466
3	2.374667053790807	3.284376937610135
4	1.2805378679515982	2.361464545537188
5	0.8269578282291634	1.9062610728777514
6	0.5764414120419162	1.5945400653717696
7	0.40071099679778055	1.2931780171033842
8	0.3368021896782874	1.2422068096382597
9	0.27183886847214245	1.1279324092158705
>10	2.012502859071556	18.63666115432227
>50	0.22593670801617965	7.314999131128731
>100	0.16503203020312254	14.27916331961376
>500	0.004584223061197848	1.3411435587119789
>1k	6.548890087425498E-4	0.35687340981484894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1182	0.3549602846889593	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	908	0.2726767669184222	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	686	0.20600909923572425	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	615	0.1846874577696362	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	597	0.17928197120076877	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	590	0.1771798375350981	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	540	0.16216459706602201	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	506	0.15195423354705026	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	496	0.14895118545323502	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	471	0.14144356521869697	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	463	0.1390411267436448	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	459	0.13783990750611871	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	444	0.13333533536539588	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	430	0.12913106803405455	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	429	0.12883076322467304	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	428	0.1285304584152915	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	426	0.1279298487965285	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	421	0.12642832474962085	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	418	0.1255274103214763	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	407	0.12222405741827956	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	399	0.11982161894322736	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	392	0.11771948527755673	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	390	0.11711887565879367	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	380	0.11411582756497844	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	372	0.11171338908992627	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	370	0.11111277947116324	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	361	0.10841003618672954	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	353	0.10600759771167735	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	352	0.10570729290229582	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	351	0.10540698809291431	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	347	0.10420576885538822	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	345	0.10360515923662518	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	341	0.10240393999909908	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	339	0.10180333038033605	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	338	0.10150302557095452	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	334	0.10030180633342843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.0030480938152224E-4	0.0
16	0.0	0.0	0.0	3.0030480938152224E-4	0.0
17	0.0	0.0	0.0	6.006096187630445E-4	0.0
18	0.0	0.0	0.0	0.001201219237526089	0.0
19	0.0	0.0	0.0	0.001201219237526089	0.0
20	0.0	0.0	0.0	0.0015015240469076112	0.0
21	0.0	0.0	0.0	0.0033033529031967447	0.0
22	0.0	0.0	0.0	0.004504572140722834	0.0
23	0.0	0.0	0.0	0.006306400997011967	3.0030480938152224E-4
24	0.0	0.0	0.0	0.006907010615775012	3.0030480938152224E-4
25	0.0	0.0	0.0	0.00930944909082719	3.0030480938152224E-4
26	0.0	0.0	0.0	0.013513716422168502	3.0030480938152224E-4
27	0.0	0.0	0.0	0.03903962521959789	3.0030480938152224E-4
28	0.0	0.0	0.0	0.14474691812189372	3.0030480938152224E-4
29	0.0	0.0	0.0	0.29670115166894395	3.0030480938152224E-4
30	0.0	0.0	0.0	0.4952026306701302	3.0030480938152224E-4
31	0.0	0.0	0.0	1.0228381807534648	3.0030480938152224E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGC	105	0.0	29.952383	29
TTAAACC	50	9.065789E-6	25.900002	4
GGTATCA	450	0.0	25.48889	1
GCCGTCT	45	1.320456E-4	24.666666	36
TACGGCT	135	0.0	23.296297	30
TAAACCG	40	0.0019283226	23.125002	5
TACACAG	40	0.0019283226	23.125002	5
ACACGAA	50	2.69652E-4	22.2	17
CTACCGT	50	2.69652E-4	22.2	8
GCTACCG	60	3.716063E-5	21.583332	7
TACACTG	60	3.716063E-5	21.583332	5
CGCGGTA	480	0.0	21.197916	22
GCGGTAA	480	0.0	21.197916	23
CACTGTG	70	5.0839844E-6	21.142857	7
GGTAATA	490	0.0	20.765305	25
GTGCAAA	45	0.0038201876	20.555555	11
AACAACG	45	0.0038201876	20.555555	10
GCTTAAA	45	0.0038201876	20.555555	2
GGACAGC	45	0.0038201876	20.555555	6
GCGTAGA	45	0.0038201876	20.555555	1
>>END_MODULE