FastQCFastQC Report
Fri 10 Feb 2017
ERR1632522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13067
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA420.3214203719292875No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT370.28315604193770566TruSeq Adapter, Index 1 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA360.27550317593938933No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA310.23723884594780745No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC260.1989745159562256No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA250.19132164995790923No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT240.18366878395959285No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC230.1760159179612765No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC220.16836305196296011No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT200.15305731996632738No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC190.14540445396801102No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT190.14540445396801102No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA190.14540445396801102No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC180.13775158796969467No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT180.13775158796969467No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG180.13775158796969467No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA180.13775158796969467No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC170.13009872197137828No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC170.13009872197137828No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC170.13009872197137828No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC170.13009872197137828No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA170.13009872197137828No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT160.12244585597306192No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA160.12244585597306192No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA160.12244585597306192No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA160.12244585597306192No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG160.12244585597306192No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA160.12244585597306192No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA150.11479298997474553No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC150.11479298997474553RNA PCR Primer, Index 1 (95% over 22bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG150.11479298997474553No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA150.11479298997474553No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC150.11479298997474553No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA140.10714012397642918No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA140.10714012397642918No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC140.10714012397642918No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC140.10714012397642918No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA140.10714012397642918No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT140.10714012397642918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT450.00362980720.55555537
GTCTCTT956.2451305E-515.57894637
TGTCTCT1102.1780113E-413.45454536
CTGTCTC1308.920086E-411.38461535
Produced by FastQC (version 0.11.5)