FastQCFastQC Report
Fri 10 Feb 2017
ERR1632521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495634
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA21100.4257173640226457No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15410.31091490898525925No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12100.24413175851535607No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11260.22718376866800905No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11070.22335029477396628No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10340.20862168454948612No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10080.2033758781681644No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9590.19348955075721197No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT9530.19227898005383004No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT9430.19026136221486015No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9180.18521731761743546No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC8800.17755036982934988No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8700.17553275199038001No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8550.17250632523192516No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7920.1597953328464149No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7880.15898828571082696No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7610.15354071754560825No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7570.1527336704100203No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7400.1493037200837715No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC7370.14869843473208053No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7190.14506672262193473No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7170.14466319905414077No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7020.14163677229568594No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6840.13800506018554012No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6830.13780329840164315No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6410.12932930347796964No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6310.12731168563899975No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6220.12549582958392685No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6220.12549582958392685No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6180.1246887824483389No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5770.11641654930856238No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5760.11621478752466537No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5710.11520597860518043No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5680.11460069325348948No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5630.11359188433400452No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5560.1121795518467256No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5550.11197779006282861No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5340.10774079260099186No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5300.1069337454654039No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5290.10673198368150692No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5270.10632846011371296No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5070.10229322443577318No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5040.1016879390840822No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5020.10128441551628822No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4990.10067913016459727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTTA303.5965498E-430.83333424
ACTGTAG250.005493364829.5999988
AGTAGGA250.005493364829.59999832
ATTACCT405.933417E-527.7533
GGTATCA9050.025.7569071
CTTAAAC551.8998291E-523.5454543
GCTTAAA551.8998291E-523.5454542
TACGGCT1750.023.25714330
ATACGGC1750.023.25714329
TAAACCG652.6777343E-622.769235
TCCCATG652.6777343E-622.769232
AAACGGT502.6990983E-422.19999927
ACGGTAG502.6990983E-422.19999929
GTATCAA10950.021.1187212
GGGCTAA806.943683E-720.81251
TTCGTGT909.450923E-820.55555524
GTAGCTA450.00382278420.55555532
CGTGTTT909.450923E-820.55555526
GGGGCGT450.00382278420.5555556
GCGGTAA8300.020.28313323
Produced by FastQC (version 0.11.5)