##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632521.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495634
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.026789929665846	34.0	33.0	34.0	31.0	34.0
2	33.16754702058374	34.0	33.0	34.0	31.0	34.0
3	33.23130374429518	34.0	33.0	34.0	31.0	34.0
4	36.51665140002502	37.0	37.0	37.0	35.0	37.0
5	36.48750489272326	37.0	37.0	37.0	35.0	37.0
6	36.53811481859598	37.0	37.0	37.0	35.0	37.0
7	36.54375809569158	37.0	37.0	37.0	35.0	37.0
8	36.515923039985154	37.0	37.0	37.0	35.0	37.0
9	38.31237969953635	39.0	39.0	39.0	37.0	39.0
10	38.34267221377065	39.0	39.0	39.0	37.0	39.0
11	38.40910026350089	39.0	39.0	39.0	37.0	39.0
12	38.36646396332778	39.0	39.0	39.0	37.0	39.0
13	38.40066258569832	39.0	39.0	39.0	37.0	39.0
14	39.93262366988544	41.0	40.0	41.0	38.0	41.0
15	39.94016552536751	41.0	40.0	41.0	38.0	41.0
16	39.88961209279428	41.0	40.0	41.0	38.0	41.0
17	39.89821521525965	41.0	40.0	41.0	38.0	41.0
18	39.904566676216724	41.0	40.0	41.0	38.0	41.0
19	39.90212535863157	41.0	40.0	41.0	38.0	41.0
20	39.8839748685522	41.0	40.0	41.0	38.0	41.0
21	39.841449537360226	41.0	40.0	41.0	38.0	41.0
22	39.807049153205796	41.0	40.0	41.0	38.0	41.0
23	39.772697191879494	41.0	40.0	41.0	38.0	41.0
24	39.755646303522354	41.0	40.0	41.0	38.0	41.0
25	39.714375930626225	41.0	40.0	41.0	38.0	41.0
26	39.61941472941727	41.0	40.0	41.0	37.0	41.0
27	39.508706020975154	41.0	39.0	41.0	37.0	41.0
28	39.46543618880061	41.0	39.0	41.0	37.0	41.0
29	39.41526610361678	41.0	39.0	41.0	37.0	41.0
30	39.39282010515824	41.0	39.0	41.0	36.0	41.0
31	39.31458697345219	41.0	39.0	41.0	36.0	41.0
32	39.27054035840963	41.0	39.0	41.0	36.0	41.0
33	39.19915703926688	41.0	39.0	41.0	35.0	41.0
34	39.15702514355351	41.0	39.0	41.0	35.0	41.0
35	39.07578979650307	41.0	39.0	41.0	35.0	41.0
36	39.01786600596408	40.0	39.0	41.0	35.0	41.0
37	38.96151999257517	40.0	39.0	41.0	35.0	41.0
38	38.88254034226869	40.0	39.0	41.0	35.0	41.0
39	38.77121827800352	40.0	38.0	41.0	35.0	41.0
40	38.688201374401274	40.0	38.0	41.0	35.0	41.0
41	38.63038653522559	40.0	38.0	41.0	35.0	41.0
42	38.558765137177836	40.0	38.0	41.0	35.0	41.0
43	37.81043875117526	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	7.0
19	16.0
20	24.0
21	37.0
22	99.0
23	166.0
24	295.0
25	411.0
26	615.0
27	844.0
28	1156.0
29	1619.0
30	2228.0
31	3004.0
32	3873.0
33	5188.0
34	8168.0
35	12743.0
36	19722.0
37	38002.0
38	101921.0
39	295493.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.10095756142637	16.26220154388117	12.43054350589346	28.206297388798994
2	22.391119253319992	19.509355693919304	30.921002191132974	27.178522861627734
3	22.11450384759722	19.19521259639169	28.68003405738912	30.010249498621967
4	16.05842214214521	15.889144005455638	34.57531162107523	33.47712223132392
5	17.825653607298932	32.465085123296625	32.23083969219222	17.478421577212217
6	33.2170512918807	33.985158403176534	15.577220287550894	17.220570017391864
7	29.512906701315888	27.09701110093335	19.5095574557032	23.88052474204756
8	27.125459512462825	29.75925783945411	20.630747688818765	22.484534959264295
9	28.13547900265115	12.433771694435814	17.144102301294907	42.28664700161813
10	20.136834841838937	21.917180822945966	28.738948498287044	29.207035836928057
11	40.47038742297744	18.94462446079163	18.26085377516474	22.32413434106619
12	22.796862200736832	23.145103039743038	26.523402349314214	27.534632410205916
13	36.63328181682451	17.198577982947093	19.60458725591868	26.563552944309716
14	24.266898558210293	19.844078493404407	22.5577744868189	33.331248461566396
15	30.449484902165715	23.434631199635213	19.038847213871527	27.07703668432755
16	25.628790599514968	23.47518531819851	22.27369389509194	28.622330187194585
17	27.639548537832354	23.073477606459605	20.907363094541537	28.379610761166507
18	27.005209489260217	20.234689307028976	22.8460920760077	29.914009127703107
19	28.490983265877645	22.16655838784264	22.698402450195104	26.644055896084613
20	30.704915320579296	19.914695117768353	21.981542832009104	27.398846729643246
21	28.06445885471941	21.314720136229557	21.289499913242434	29.331321095808597
22	28.934657428667123	22.372153645633674	20.354939330231584	28.338249595467623
23	29.104742612492284	21.184180262048205	21.430531400186428	28.280545725273083
24	28.57975845079232	20.596246423772378	21.864924520916645	28.959070604518654
25	29.306706158173167	21.563492415774544	21.734989932086986	27.394811493965303
26	29.165674671229176	22.062651069135693	21.267709640581558	27.503964619053576
27	27.825976426153172	21.230181948776718	23.891621640161894	27.05221998490822
28	26.792754330816692	22.862233018719458	20.9565929698124	29.388419680651452
29	27.779369454072967	23.342627826178187	21.272551923395085	27.605450796353757
30	27.3750388391434	21.446067057546497	23.60572519237986	27.57316891093024
31	28.931429240124768	21.666996210913698	21.790676184442553	27.61089836451898
32	26.12633515860494	21.454137528902375	22.064466925190764	30.35506038730192
33	26.649099940682035	21.400670656169673	23.581110254744427	28.369119148403865
34	26.526832299640464	21.308869044496547	23.208859763454484	28.955438892408512
35	26.336167413857808	23.364418098839064	23.648095166998228	26.6513193203049
36	26.863976240532327	21.607072961096293	24.159561289177095	27.369389509194285
37	27.749710471840107	21.23260309018348	23.55024070180819	27.467445736168223
38	26.517349495797305	20.647292155098317	24.991828647752172	27.84352970135221
39	25.71211821626442	19.641711424155726	26.153774761214926	28.492395598364922
40	25.59812280836262	20.698539648208154	27.006420059963602	26.696917483465622
41	23.773994520149948	19.74198703075253	27.947033496491365	28.536984952606158
42	21.904469830560455	20.647493916882215	29.648490620094663	27.799545632462664
43	21.21202338822599	21.501148024550375	28.494614977987787	28.792213609235844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.0
2	3.0
3	12.0
4	21.0
5	21.0
6	26.0
7	31.0
8	24.5
9	18.0
10	30.0
11	42.0
12	42.0
13	93.0
14	144.0
15	232.0
16	320.0
17	303.0
18	286.0
19	286.0
20	357.0
21	428.0
22	358.5
23	289.0
24	353.0
25	417.0
26	417.0
27	524.5
28	632.0
29	821.0
30	1010.0
31	1314.0
32	1618.0
33	1618.0
34	2337.5
35	3057.0
36	3478.0
37	3899.0
38	5635.5
39	7372.0
40	7372.0
41	9392.0
42	11412.0
43	14392.0
44	17372.0
45	25607.0
46	33842.0
47	33842.0
48	39811.5
49	45781.0
50	51321.5
51	56862.0
52	59090.0
53	61318.0
54	61318.0
55	57980.0
56	54642.0
57	53576.5
58	52511.0
59	48409.0
60	44307.0
61	44307.0
62	39613.5
63	34920.0
64	27810.5
65	20701.0
66	17870.0
67	15039.0
68	15039.0
69	12769.5
70	10500.0
71	8609.5
72	6719.0
73	5246.0
74	3773.0
75	3773.0
76	2989.5
77	2206.0
78	1831.5
79	1457.0
80	1269.0
81	1081.0
82	1081.0
83	928.0
84	775.0
85	558.5
86	342.0
87	313.0
88	284.0
89	284.0
90	196.5
91	109.0
92	88.0
93	67.0
94	41.0
95	15.0
96	15.0
97	10.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	495634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.59017501418866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.02147659759967	34.10446440047127
2	7.4630961415858135	6.058567570693684
3	2.3907322475803534	2.9112072102405344
4	1.2706324282193002	2.063007705604996
5	0.7904079150571779	1.6041397802385404
6	0.5664709323063929	1.3795892569660262
7	0.44120297311248763	1.2535954126791373
8	0.32078613681969254	1.041661234850943
9	0.2586389107075846	0.9448378785989977
>10	1.9710892247561789	16.529257992312758
>50	0.2707142675913167	7.597970469655803
>100	0.21277542065295107	17.552351825156343
>500	0.018480494282063827	5.10714502497792
>1k	0.0034963097290391025	1.852204237553026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2110	0.4257173640226457	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1541	0.31091490898525925	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1210	0.24413175851535607	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1126	0.22718376866800905	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1107	0.22335029477396628	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1034	0.20862168454948612	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1008	0.2033758781681644	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	959	0.19348955075721197	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	953	0.19227898005383004	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	943	0.19026136221486015	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	918	0.18521731761743546	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	880	0.17755036982934988	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	870	0.17553275199038001	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	855	0.17250632523192516	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	792	0.1597953328464149	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	788	0.15898828571082696	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	761	0.15354071754560825	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	757	0.1527336704100203	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	740	0.1493037200837715	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	737	0.14869843473208053	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	719	0.14506672262193473	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	717	0.14466319905414077	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	702	0.14163677229568594	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	684	0.13800506018554012	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	683	0.13780329840164315	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	641	0.12932930347796964	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	631	0.12731168563899975	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	622	0.12549582958392685	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	622	0.12549582958392685	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	618	0.1246887824483389	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	577	0.11641654930856238	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	576	0.11621478752466537	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	571	0.11520597860518043	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	568	0.11460069325348948	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	563	0.11359188433400452	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	556	0.1121795518467256	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	555	0.11197779006282861	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	534	0.10774079260099186	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	530	0.1069337454654039	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	529	0.10673198368150692	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	527	0.10632846011371296	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	507	0.10229322443577318	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	504	0.1016879390840822	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	502	0.10128441551628822	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	499	0.10067913016459727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	2.017617838969885E-4	0.0	0.0
12	0.0	0.0	2.017617838969885E-4	0.0	0.0
13	0.0	0.0	2.017617838969885E-4	0.0	0.0
14	0.0	0.0	2.017617838969885E-4	0.0	0.0
15	2.017617838969885E-4	0.0	2.017617838969885E-4	2.017617838969885E-4	0.0
16	2.017617838969885E-4	0.0	2.017617838969885E-4	4.03523567793977E-4	0.0
17	2.017617838969885E-4	0.0	2.017617838969885E-4	0.001614094271175908	0.0
18	2.017617838969885E-4	0.0	2.017617838969885E-4	0.0018158560550728966	0.0
19	2.017617838969885E-4	0.0	2.017617838969885E-4	0.002421141406763862	0.0
20	4.03523567793977E-4	0.0	2.017617838969885E-4	0.0030264267584548276	0.0
21	4.03523567793977E-4	0.0	2.017617838969885E-4	0.004640521029630735	0.0
22	4.03523567793977E-4	0.0	2.017617838969885E-4	0.00807047135587954	0.0
23	4.03523567793977E-4	0.0	2.017617838969885E-4	0.00928104205926147	0.0
24	4.03523567793977E-4	0.0	2.017617838969885E-4	0.011500421682128345	0.0
25	4.03523567793977E-4	0.0	2.017617838969885E-4	0.01351803952109823	0.0
26	4.03523567793977E-4	0.0	2.017617838969885E-4	0.018158560550728967	0.0
27	4.03523567793977E-4	0.0	2.017617838969885E-4	0.03833473894042782	0.0
28	4.03523567793977E-4	0.0	2.017617838969885E-4	0.13800506018554012	0.0
29	4.03523567793977E-4	0.0	2.017617838969885E-4	0.2842823535108568	0.0
30	4.03523567793977E-4	0.0	2.017617838969885E-4	0.47676309534858385	0.0
31	4.03523567793977E-4	0.0	2.017617838969885E-4	0.9420257690150393	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTTA	30	3.5965498E-4	30.833334	24
ACTGTAG	25	0.0054933648	29.599998	8
AGTAGGA	25	0.0054933648	29.599998	32
ATTACCT	40	5.933417E-5	27.75	33
GGTATCA	905	0.0	25.756907	1
CTTAAAC	55	1.8998291E-5	23.545454	3
GCTTAAA	55	1.8998291E-5	23.545454	2
TACGGCT	175	0.0	23.257143	30
ATACGGC	175	0.0	23.257143	29
TAAACCG	65	2.6777343E-6	22.76923	5
TCCCATG	65	2.6777343E-6	22.76923	2
AAACGGT	50	2.6990983E-4	22.199999	27
ACGGTAG	50	2.6990983E-4	22.199999	29
GTATCAA	1095	0.0	21.118721	2
GGGCTAA	80	6.943683E-7	20.8125	1
TTCGTGT	90	9.450923E-8	20.555555	24
GTAGCTA	45	0.003822784	20.555555	32
CGTGTTT	90	9.450923E-8	20.555555	26
GGGGCGT	45	0.003822784	20.555555	6
GCGGTAA	830	0.0	20.283133	23
>>END_MODULE