FastQCFastQC Report
Fri 10 Feb 2017
ERR1632520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50292
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2490.49510856597470776No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1860.36984013361966117No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1840.3658633579893422No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1620.322118826055834No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1570.3121768869800366No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1540.30621172353455817No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1510.3002465600890798No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1480.29428139664360137No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1460.2903046210132824No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1240.24656008907977411No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1220.2425833134494552No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1210.2405949256342957No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1160.23065298655849836No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1130.22468782311301996No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1100.21872265966754156No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1070.21275749622206314No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1060.2107691084069037No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1060.2107691084069037No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1040.20679233277658476No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1020.2028155571462658No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC950.18889684244014954No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG940.18690845462499006No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT920.1829316789946711No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC860.1710013521037143No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC850.16901296428855483No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG850.16901296428855483No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT820.16304780084307643No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC800.1590710252127575No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG790.157082637397598No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA770.15310586176727908No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT750.14912908613696013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT740.14714069832180068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT740.14714069832180068No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC740.14714069832180068No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA670.1332219836156844No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA660.13123359580052493No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT650.12924520798536546No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC640.127256820170206No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG640.127256820170206No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC640.127256820170206No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA620.12328004453988706No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG620.12328004453988706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT620.12328004453988706No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT610.1212916567247276No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC610.1212916567247276No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC600.11930326890956812No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA600.11930326890956812No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT590.11731488109440866No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT590.11731488109440866No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC580.11532649327924918No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC580.11532649327924918No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT580.11532649327924918RNA PCR Primer, Index 46 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG580.11532649327924918No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT570.11333810546408972No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA550.10936132983377078No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC550.10936132983377078No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC550.10936132983377078No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA540.10737294201861132No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA540.10737294201861132No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG530.10538455420345184No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG530.10538455420345184No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG520.10339616638829238No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC520.10339616638829238No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG520.10339616638829238No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG510.1014077785731329No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA510.1014077785731329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGC200.001825238837.021
GCCTTAT200.001825238837.026
TGATTCC200.001825238837.017
GTGATTC200.001825238837.016
GATTCCT200.001825238837.018
ATTCCTC200.001825238837.019
TCAATGA303.5511487E-430.83333232
GATATGC250.005447087329.59999834
TTATTGA250.005447087329.59999829
TTCTGCG502.6342968E-729.59999818
TATTGAT250.005447087329.59999830
TCTGCGG502.6342968E-729.59999819
CTGCGGG502.6342968E-729.59999820
TTCTTCT502.6342968E-729.59999815
CTTATTG250.005447087329.59999828
ATTGATA250.005447087329.59999831
ATATGCC250.005447087329.59999835
CCTTATT250.005447087329.59999827
TGGGCAG250.005447087329.5999986
CTTCTGC502.6342968E-729.59999817
Produced by FastQC (version 0.11.5)