##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632520.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50292
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.972600015907105	34.0	33.0	34.0	31.0	34.0
2	33.11574405472043	34.0	33.0	34.0	31.0	34.0
3	33.142090193271294	34.0	33.0	34.0	31.0	34.0
4	36.45816432036904	37.0	37.0	37.0	35.0	37.0
5	36.46577984570111	37.0	37.0	37.0	35.0	37.0
6	36.52706195816432	37.0	37.0	37.0	35.0	37.0
7	36.53571144516027	37.0	37.0	37.0	35.0	37.0
8	36.49363715899149	37.0	37.0	37.0	35.0	37.0
9	38.31092420265649	39.0	39.0	39.0	37.0	39.0
10	38.34454784061083	39.0	39.0	39.0	37.0	39.0
11	38.41962936451125	39.0	39.0	39.0	37.0	39.0
12	38.363616479758214	39.0	39.0	39.0	37.0	39.0
13	38.41362443330947	39.0	39.0	39.0	37.0	39.0
14	39.869104430128054	41.0	40.0	41.0	38.0	41.0
15	39.87578541318699	41.0	40.0	41.0	38.0	41.0
16	39.779388371908055	41.0	40.0	41.0	38.0	41.0
17	39.838105464089715	41.0	40.0	41.0	38.0	41.0
18	39.88688061719558	41.0	40.0	41.0	38.0	41.0
19	39.89722023383441	41.0	40.0	41.0	38.0	41.0
20	39.892686709615845	41.0	40.0	41.0	38.0	41.0
21	39.83890081921578	41.0	40.0	41.0	38.0	41.0
22	39.808518253400145	41.0	40.0	41.0	38.0	41.0
23	39.74329913306291	41.0	40.0	41.0	38.0	41.0
24	39.7194782470373	41.0	40.0	41.0	38.0	41.0
25	39.69255547602004	41.0	40.0	41.0	38.0	41.0
26	39.59504493756462	41.0	40.0	41.0	37.0	41.0
27	39.463373896444764	41.0	39.0	41.0	37.0	41.0
28	39.42925316153663	41.0	39.0	41.0	37.0	41.0
29	39.382466396245924	41.0	39.0	41.0	36.0	41.0
30	39.33991489700151	41.0	39.0	41.0	36.0	41.0
31	39.26755746440786	41.0	39.0	41.0	36.0	41.0
32	39.21432832259604	41.0	39.0	41.0	35.0	41.0
33	39.12880776266603	41.0	39.0	41.0	35.0	41.0
34	39.08796627694265	41.0	39.0	41.0	35.0	41.0
35	38.98480871709218	40.0	39.0	41.0	35.0	41.0
36	38.937982184045175	40.0	39.0	41.0	35.0	41.0
37	38.87644158116599	40.0	39.0	41.0	35.0	41.0
38	38.81404597152628	40.0	38.0	41.0	35.0	41.0
39	38.67316869482224	40.0	38.0	41.0	35.0	41.0
40	38.59707309313608	40.0	38.0	41.0	35.0	41.0
41	38.52730056470214	40.0	38.0	41.0	35.0	41.0
42	38.428239083750896	40.0	37.0	41.0	35.0	41.0
43	37.67611150878867	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	3.0
20	2.0
21	7.0
22	13.0
23	17.0
24	27.0
25	51.0
26	73.0
27	94.0
28	121.0
29	162.0
30	244.0
31	296.0
32	402.0
33	579.0
34	815.0
35	1262.0
36	2133.0
37	4186.0
38	10739.0
39	29064.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.014793605344785	14.928815716217292	13.857074683846339	28.199315994591583
2	23.262149049550622	19.31321084864392	30.680823987910603	26.74381611389485
3	19.766563270500278	18.468146027201147	31.41851586733477	30.346774834963814
4	13.88888888888889	17.535592141891353	35.74922452875209	32.82629444046767
5	15.511413346059017	33.295553964845304	33.848325777459635	17.344706911636045
6	28.644714865187304	36.351706036745405	16.487711763302315	18.515867334764973
7	28.02831464248787	28.06211723534558	19.444444444444446	24.465123677722104
8	27.42980991012487	28.791855563509106	21.18627217052414	22.592062355841882
9	28.404119939553013	11.045494313210849	16.57917760279965	43.97120814443649
10	21.391076115485564	21.206156048675734	25.97431002942814	31.428457806410563
11	40.113735783027124	19.852063946552136	16.487711763302315	23.54648850711843
12	21.921975662133143	26.795514197089	24.532728863437526	26.749781277340333
13	39.45160264057902	17.11206553726239	18.21760916249105	25.21872265966754
14	23.572337548715502	21.37318062514913	23.289986478962856	31.76449534717251
15	32.30334844508073	23.136880617195576	18.287202736021634	26.27256820170206
16	22.87640181340969	24.1609003420027	23.403324584426947	29.55937326016066
17	28.535353535353536	23.53654656804263	19.927622683528195	28.000477213075637
18	28.66658713115406	18.70874095283544	20.70309393144039	31.92157798457011
19	31.567644953471724	21.196214109599936	21.601845223892468	25.634295713035872
20	34.61385508629603	18.462180863755666	20.327288634375247	26.596675415573053
21	30.322914181181897	19.830191680585383	19.83615684403086	30.01073729420186
22	30.080330867732442	21.95776664280601	19.442456056629283	28.519446432832257
23	29.41620933746918	21.049073411278137	20.62355841883401	28.911158832418675
24	28.18937405551579	18.955301041915217	24.043585460908297	28.8117394416607
25	29.984888252604787	21.062992125984252	21.8503937007874	27.101725920623558
26	28.29873538534956	22.7531217688698	21.794718841962936	27.153424003817705
27	27.53519446432832	20.058856279328722	24.821045096635647	27.584904159707307
28	26.890956812216654	22.64773721466635	20.8840372226199	29.577268750497097
29	28.042233357193986	24.0237015827567	20.756780402449692	27.17728465759962
30	26.94663167104112	20.8840372226199	24.681857949574486	27.487473156764498
31	28.00843076433628	22.104907341127813	23.248230334844507	26.6384315596914
32	25.602481507993318	20.788594607492243	23.295951642408337	30.312972242106103
33	25.81722739203054	20.9675495108566	25.50306211723535	27.712160979877513
34	26.46941859540285	21.019247594050743	24.63015986638034	27.881173944166072
35	24.874731567644954	23.46894138232721	25.554760200429495	26.101566849598345
36	26.16917203531377	21.689334287759486	26.30637079455977	25.835122882366978
37	26.274556589517218	21.546170365068004	25.578620854211405	26.600652191203373
38	24.536705639067844	19.903762029746282	27.165354330708663	28.39417800047721
39	23.7294201861131	19.140221108725044	28.78787878787879	28.342479917283065
40	23.630000795355127	20.116519525968346	29.599141016463854	26.65433866221268
41	21.683369124314005	19.060685596118667	30.71860335639863	28.537341923168697
42	18.89763779527559	19.931599459158512	33.325379782072694	27.845382963493197
43	17.869641294838146	21.89612662053607	31.458283623637957	28.775948460987834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	2.0
6	3.5
7	5.0
8	7.0
9	9.0
10	11.0
11	13.0
12	13.0
13	30.0
14	47.0
15	87.0
16	127.0
17	123.0
18	119.0
19	119.0
20	141.0
21	163.0
22	127.0
23	91.0
24	69.5
25	48.0
26	48.0
27	50.5
28	53.0
29	53.5
30	54.0
31	58.5
32	63.0
33	63.0
34	85.0
35	107.0
36	114.5
37	122.0
38	216.0
39	310.0
40	310.0
41	502.0
42	694.0
43	1035.0
44	1376.0
45	2684.5
46	3993.0
47	3993.0
48	4829.5
49	5666.0
50	6311.0
51	6956.0
52	6865.0
53	6774.0
54	6774.0
55	6091.5
56	5409.0
57	5092.5
58	4776.0
59	4575.5
60	4375.0
61	4375.0
62	3974.0
63	3573.0
64	2784.5
65	1996.0
66	1688.5
67	1381.0
68	1381.0
69	1112.5
70	844.0
71	722.5
72	601.0
73	422.0
74	243.0
75	243.0
76	195.5
77	148.0
78	118.5
79	89.0
80	66.0
81	43.0
82	43.0
83	26.0
84	9.0
85	8.5
86	8.0
87	5.5
88	3.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	50292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.6222063151197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83426831222953	40.111747395211964
2	8.246513864401345	8.184204247196373
3	3.3619169738740187	5.004772130756383
4	1.735053694502324	3.4438876958562
5	1.2141368809104023	3.012407539966595
6	0.7413046962654272	2.2071104748270103
7	0.6130790190735695	2.129563350035791
8	0.4127263984612919	1.638431559691402
9	0.30854303574290753	1.3779527559055118
>10	2.2679916653309826	21.393064503300725
>50	0.18432441096329538	5.9930008748906385
>100	0.08014104824491104	5.5038574723614095
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	249	0.49510856597470776	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	186	0.36984013361966117	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	184	0.3658633579893422	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	162	0.322118826055834	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	157	0.3121768869800366	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	154	0.30621172353455817	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	151	0.3002465600890798	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	148	0.29428139664360137	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	146	0.2903046210132824	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	124	0.24656008907977411	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	122	0.2425833134494552	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	121	0.2405949256342957	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	116	0.23065298655849836	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	113	0.22468782311301996	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	110	0.21872265966754156	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	107	0.21275749622206314	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	106	0.2107691084069037	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	106	0.2107691084069037	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	104	0.20679233277658476	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	102	0.2028155571462658	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	95	0.18889684244014954	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	94	0.18690845462499006	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	92	0.1829316789946711	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	86	0.1710013521037143	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	85	0.16901296428855483	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	85	0.16901296428855483	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	82	0.16304780084307643	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	80	0.1590710252127575	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	79	0.157082637397598	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	77	0.15310586176727908	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	75	0.14912908613696013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.14714069832180068	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.14714069832180068	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	74	0.14714069832180068	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	67	0.1332219836156844	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	66	0.13123359580052493	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	65	0.12924520798536546	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	64	0.127256820170206	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	64	0.127256820170206	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	64	0.127256820170206	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	62	0.12328004453988706	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	62	0.12328004453988706	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	62	0.12328004453988706	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT	61	0.1212916567247276	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	61	0.1212916567247276	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	60	0.11930326890956812	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	60	0.11930326890956812	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT	59	0.11731488109440866	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT	59	0.11731488109440866	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	58	0.11532649327924918	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	58	0.11532649327924918	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	58	0.11532649327924918	RNA PCR Primer, Index 46 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	58	0.11532649327924918	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	57	0.11333810546408972	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	55	0.10936132983377078	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	55	0.10936132983377078	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	55	0.10936132983377078	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	54	0.10737294201861132	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	54	0.10737294201861132	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	53	0.10538455420345184	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	53	0.10538455420345184	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	52	0.10339616638829238	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	52	0.10339616638829238	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	52	0.10339616638829238	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG	51	0.1014077785731329	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	51	0.1014077785731329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.001988387815159469	0.0
16	0.0	0.0	0.0	0.007953551260637876	0.001988387815159469
17	0.0	0.0	0.0	0.01789549033643522	0.001988387815159469
18	0.0	0.0	0.0	0.021872265966754154	0.001988387815159469
19	0.0	0.0	0.0	0.025849041597073092	0.001988387815159469
20	0.0	0.0	0.0	0.02783742941223256	0.001988387815159469
21	0.0	0.0	0.0	0.04374453193350831	0.001988387815159469
22	0.0	0.0	0.0	0.05965163445478406	0.001988387815159469
23	0.0	0.0	0.0	0.0695935735305814	0.001988387815159469
24	0.0	0.0	0.0	0.08351228823669768	0.001988387815159469
25	0.0	0.0	0.0	0.09544261512765449	0.001988387815159469
26	0.0	0.0	0.0	0.1014077785731329	0.001988387815159469
27	0.0	0.0	0.0	0.1511174739521196	0.001988387815159469
28	0.0	0.0	0.0	0.4314801558896047	0.001988387815159469
29	0.0	0.0	0.0	0.9305654974946314	0.001988387815159469
30	0.0	0.0	0.0	1.5926986399427345	0.001988387815159469
31	0.0	0.0	0.0	2.841406187862881	0.001988387815159469
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCGC	20	0.0018252388	37.0	21
GCCTTAT	20	0.0018252388	37.0	26
TGATTCC	20	0.0018252388	37.0	17
GTGATTC	20	0.0018252388	37.0	16
GATTCCT	20	0.0018252388	37.0	18
ATTCCTC	20	0.0018252388	37.0	19
TCAATGA	30	3.5511487E-4	30.833332	32
GATATGC	25	0.0054470873	29.599998	34
TTATTGA	25	0.0054470873	29.599998	29
TTCTGCG	50	2.6342968E-7	29.599998	18
TATTGAT	25	0.0054470873	29.599998	30
TCTGCGG	50	2.6342968E-7	29.599998	19
CTGCGGG	50	2.6342968E-7	29.599998	20
TTCTTCT	50	2.6342968E-7	29.599998	15
CTTATTG	25	0.0054470873	29.599998	28
ATTGATA	25	0.0054470873	29.599998	31
ATATGCC	25	0.0054470873	29.599998	35
CCTTATT	25	0.0054470873	29.599998	27
TGGGCAG	25	0.0054470873	29.599998	6
CTTCTGC	50	2.6342968E-7	29.599998	17
>>END_MODULE