FastQCFastQC Report
Fri 10 Feb 2017
ERR1632519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7673
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA480.6255701811546983No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT450.5864720448325297RNA PCR Primer, Index 27 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA340.44311221165124465No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA280.36491593900690733No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC230.2997523784699596No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA230.2997523784699596No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA220.28671966636257007No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA220.28671966636257007No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT200.260654242147791No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT200.260654242147791No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA190.2476215300404014No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC190.2476215300404014No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA190.2476215300404014No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA190.2476215300404014No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC180.23458881793301184No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG180.23458881793301184No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA180.23458881793301184No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA170.22155610582562232No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG170.22155610582562232No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT170.22155610582562232No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC170.22155610582562232No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT160.20852339371823275No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC160.20852339371823275No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC160.20852339371823275No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT150.19549068161084324No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC150.19549068161084324No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT150.19549068161084324No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC150.19549068161084324No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC150.19549068161084324RNA PCR Primer, Index 27 (95% over 24bp)
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG150.19549068161084324No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA150.19549068161084324No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC140.18245796950345367No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA140.18245796950345367No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC140.18245796950345367No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC130.16942525739606412No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG130.16942525739606412No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC130.16942525739606412No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA130.16942525739606412No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC120.15639254528867458RNA PCR Primer, Index 27 (95% over 21bp)
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG120.15639254528867458No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA120.15639254528867458No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT120.15639254528867458No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC110.14335983318128503No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC110.14335983318128503No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC110.14335983318128503No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA110.14335983318128503No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG110.14335983318128503No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG110.14335983318128503No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC100.1303271210738955No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC100.1303271210738955No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA100.1303271210738955No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT100.1303271210738955No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC100.1303271210738955No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG100.1303271210738955No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC90.11729440896650592No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA90.11729440896650592No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC90.11729440896650592No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA90.11729440896650592No Hit
CTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA90.11729440896650592No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG90.11729440896650592No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA90.11729440896650592No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC90.11729440896650592No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT80.10426169685911638No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC80.10426169685911638No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG80.10426169685911638No Hit
CTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGA80.10426169685911638No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA80.10426169685911638No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT80.10426169685911638No Hit
GGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGC80.10426169685911638No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA80.10426169685911638No Hit
TTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCAT80.10426169685911638No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA80.10426169685911638No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA80.10426169685911638No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT80.10426169685911638No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG80.10426169685911638No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC80.10426169685911638No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTC80.10426169685911638No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA80.10426169685911638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGT352.0952562E-531.7142872
TTTAGTG352.0952562E-531.7142873
TCTTTAG352.0952562E-531.7142871
TAGTGAC303.2790177E-430.8333345
TGACCAT303.2790177E-430.8333348
AGTGACC303.2790177E-430.8333346
GTGACCA303.2790177E-430.8333347
GACCATG303.2790177E-430.8333349
TTAGTGA303.2790177E-430.8333344
GTGCCAG303.2790177E-430.83333411
GCGGTAA250.00516606729.623
ACTCCGT250.00516606729.66
AACTCCG250.00516606729.65
GCAGCCG250.00516606729.617
TAACTCC250.00516606729.64
CTAACTC250.00516606729.63
GCTAACT250.00516606729.62
TGCCAGC250.00516606729.612
CGCGGTA250.00516606729.622
CGTGCCA250.00516606729.610
Produced by FastQC (version 0.11.5)