FastQCFastQC Report
Fri 10 Feb 2017
ERR1632518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153080
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9110.5951136660569636No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6570.4291873530180298No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5240.34230467729291875No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4170.2724065847922655No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4050.2645675463809773No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3870.25280898876404495No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3770.2462764567546381No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3560.23255813953488372No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3520.22994512673112097No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3500.2286386203292396No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3440.22471910112359553No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3430.22406584792265483No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3380.2207995819179514No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3230.21100078390384114No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3210.20969427750195976No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3210.20969427750195976No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3110.2031617454925529No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3020.19728246668408675No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2960.19336294747844265No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2850.1861771622680951No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2830.18487065586621373No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2720.17768487065586622No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2710.17703161745492554No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2680.17507185785210347No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2600.169845832244578No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2520.1646198066370525No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT2490.16266004703423048Illumina PCR Primer Index 10 (95% over 22bp)
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2480.16200679383328978No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2410.15743402142670498No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2380.15547426182388294No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2360.1541677554220016No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2280.1489417298144761No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2190.14306245100600992No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2140.1397961850013065No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2140.1397961850013065No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2140.1397961850013065No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2060.13457015939378103No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2050.13391690619284036No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2040.13326365299189966No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1920.12542461458061144No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1920.12542461458061144No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1910.12477136137967076No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG1850.12085184217402667No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1820.11889208257120461No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1820.11889208257120461No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1820.11889208257120461No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC1760.11497256336556048No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1750.1143193101646198No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1740.11366605696367911No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1740.11366605696367911No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1730.11301280376273842No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1710.11170629736085708No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1710.11170629736085708No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1700.11105304415991639No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1680.10974653775803501No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1680.10974653775803501No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC1640.10713352495427227No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1620.1058270185523909No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1600.10452051215050953No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1600.10452051215050953No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1590.10386725894956886No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1580.10321400574862817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2300.031.3695661
TGGAGAG250.005481653429.5999985
CACGAGA502.6947964E-729.59999811
CTGAAGG250.005481653429.5999984
CTGTTTG250.005481653429.5999989
GCCTATC502.6947964E-729.59999823
TCTCCGA502.6947964E-729.5999981
AGCCTAT502.6947964E-729.59999822
AAAGACT250.005481653429.59999836
TACCGCT250.005481653429.59999835
TATCTCG604.2715328E-827.74999826
ATCTCGT556.2023355E-726.9090927
CTATCTC556.2023355E-726.9090925
TCGTATG556.2023355E-726.9090930
GCCGTCT556.2023355E-726.9090936
CACAGCT358.834167E-426.4285734
GAGCCTA659.2810296E-825.61538521
CTCGTAT601.3247936E-624.66666629
AGCCCAC601.3247936E-624.6666667
TATGCCG601.3247936E-624.66666633
Produced by FastQC (version 0.11.5)