##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632518.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153080
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.99519858897309	34.0	33.0	34.0	31.0	34.0
2	33.13153253200941	34.0	33.0	34.0	31.0	34.0
3	33.198308074209564	34.0	33.0	34.0	31.0	34.0
4	36.489763522341256	37.0	37.0	37.0	35.0	37.0
5	36.46767049908544	37.0	37.0	37.0	35.0	37.0
6	36.510739482623464	37.0	37.0	37.0	35.0	37.0
7	36.527639142931804	37.0	37.0	37.0	35.0	37.0
8	36.49486542984061	37.0	37.0	37.0	35.0	37.0
9	38.30580742095636	39.0	39.0	39.0	37.0	39.0
10	38.323151293441335	39.0	39.0	39.0	37.0	39.0
11	38.392879540109746	39.0	39.0	39.0	37.0	39.0
12	38.336588711784685	39.0	39.0	39.0	37.0	39.0
13	38.38695453357722	39.0	39.0	39.0	37.0	39.0
14	39.886307812908285	41.0	40.0	41.0	38.0	41.0
15	39.90118892082571	41.0	40.0	41.0	38.0	41.0
16	39.83211392735824	41.0	40.0	41.0	38.0	41.0
17	39.8558335510844	41.0	40.0	41.0	38.0	41.0
18	39.869408152599945	41.0	40.0	41.0	38.0	41.0
19	39.87003527567285	41.0	40.0	41.0	38.0	41.0
20	39.85372354324536	41.0	40.0	41.0	38.0	41.0
21	39.80099947739744	41.0	40.0	41.0	38.0	41.0
22	39.7682649594983	41.0	40.0	41.0	38.0	41.0
23	39.73856153645153	41.0	40.0	41.0	38.0	41.0
24	39.721544290567024	41.0	40.0	41.0	38.0	41.0
25	39.67903057224981	41.0	40.0	41.0	37.0	41.0
26	39.59136399268356	41.0	40.0	41.0	37.0	41.0
27	39.44370917167494	41.0	39.0	41.0	37.0	41.0
28	39.428462241964986	41.0	39.0	41.0	37.0	41.0
29	39.368068983538016	41.0	39.0	41.0	36.0	41.0
30	39.33539325842697	41.0	39.0	41.0	36.0	41.0
31	39.26295401097465	41.0	39.0	41.0	36.0	41.0
32	39.219564933368176	41.0	39.0	41.0	36.0	41.0
33	39.13398223151293	41.0	39.0	41.0	35.0	41.0
34	39.1063888163052	41.0	39.0	41.0	35.0	41.0
35	39.01065455970734	40.0	39.0	41.0	35.0	41.0
36	38.956362686177165	40.0	39.0	41.0	35.0	41.0
37	38.8871505095375	40.0	39.0	41.0	35.0	41.0
38	38.80243663443951	40.0	38.0	41.0	35.0	41.0
39	38.67034230467729	40.0	38.0	41.0	35.0	41.0
40	38.5891625293964	40.0	38.0	41.0	35.0	41.0
41	38.51401881369219	40.0	38.0	41.0	35.0	41.0
42	38.43882937026392	40.0	38.0	41.0	35.0	41.0
43	37.6832636529919	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	0.0
18	2.0
19	2.0
20	6.0
21	11.0
22	31.0
23	45.0
24	94.0
25	141.0
26	204.0
27	267.0
28	355.0
29	529.0
30	747.0
31	1003.0
32	1261.0
33	1739.0
34	2510.0
35	4108.0
36	6428.0
37	12467.0
38	32697.0
39	88431.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.10491246407107	15.69571465900183	12.67245884504834	28.526914031878757
2	22.830546119675986	19.623726156258165	30.442905670237785	27.102822053828064
3	22.068199634178207	18.581787300757775	29.155996864384637	30.194016200679386
4	15.629736085706819	16.456101384896783	35.203814998693495	32.7103475307029
5	17.81682780245623	32.093023255813954	32.2158348575908	17.874314084139012
6	31.99699503527567	34.13247974915077	16.1046511627907	17.76587405278286
7	29.168408675202507	26.972171413639927	19.175594460412857	24.68382545074471
8	26.72850796968905	28.872484975176377	21.20067938332898	23.19832767180559
9	27.933760125424616	11.964332375228638	17.015286124902012	43.08662137444473
10	20.52260256075255	21.669061928403448	27.514371570420693	30.293963940423307
11	40.888424353279326	18.485105827018554	18.00300496472433	22.62346485497779
12	22.36412333420434	23.25254768748367	26.4515286124902	27.93180036582179
13	37.92788084661615	16.83172197543768	19.02599947739744	26.21439770054873
14	24.113535406323493	20.1894434282728	22.67964463025869	33.01737653514502
15	31.116409720407628	23.012150509537495	18.792788084661616	27.078651685393258
16	24.996080480794355	23.689574078912987	22.646328717010714	28.66801672328194
17	28.02586882675725	22.940945910634962	20.432453619022734	28.600731643585053
18	27.324274888946952	19.517245884504835	22.43075516070029	30.727724065847923
19	29.059315390645413	21.746145806114452	22.620851842174027	26.573686961066112
20	32.13221844787039	19.042330807420957	21.257512411810815	27.56793833289783
21	28.504050169845836	20.62842957930494	21.224849751763784	29.642670499085444
22	29.22589495688529	21.998954794878493	20.047034230467727	28.728116017768485
23	29.962111314345442	20.386072641755945	21.207211915338387	28.44460412856023
24	28.59681212437941	19.86804285340998	22.120459890253464	29.414685131957146
25	29.69754376796446	21.08897308596812	21.6971518160439	27.51633133002352
26	29.456493336817353	21.80493859419911	21.316958453096422	27.421609615887117
27	27.288345962895217	21.148419127253725	24.38920825712046	27.1740266527306
28	27.055787823360333	22.942905670237785	20.721844787039455	29.279461719362427
29	27.905016984583224	23.592892605173766	20.970734256597858	27.531356153645152
30	27.4438202247191	20.91651424091978	23.979618500130652	27.660047034230466
31	29.38071596550823	21.475698980925007	21.62268095113666	27.520904102430098
32	26.215050953749675	20.748628168278024	22.30990331852626	30.72641755944604
33	26.67820747321662	20.995557878233605	23.85419388555004	28.47204076299974
34	26.676247713613797	21.3953488372093	23.25320094068461	28.67520250849229
35	25.881891821269924	23.528220538280635	23.76143193101646	26.828455709432973
36	27.190357982754115	21.02038149986935	24.56036059576692	27.228899921609617
37	27.62607786778155	20.9152077345179	23.70133263652992	27.75738176117063
38	26.13535406323491	20.118892082571204	25.647373922132218	28.09837993206167
39	25.84008361640972	18.999869349359813	26.416252939639406	28.743794094591063
40	25.719885027436636	20.047034230467727	27.711000783903838	26.522079958191796
41	23.50078390384113	19.235040501698457	28.563496211131433	28.700679383328982
42	21.142539848445256	20.60948523647766	30.049647243271494	28.19832767180559
43	20.36190227332114	21.429318003658217	28.975045727724062	29.23373399529658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	1.0
4	2.0
5	2.0
6	4.0
7	6.0
8	8.5
9	11.0
10	15.0
11	19.0
12	19.0
13	35.0
14	51.0
15	90.0
16	129.0
17	129.0
18	129.0
19	129.0
20	166.0
21	203.0
22	156.0
23	109.0
24	115.5
25	122.0
26	122.0
27	138.5
28	155.0
29	189.0
30	223.0
31	254.5
32	286.0
33	286.0
34	489.0
35	692.0
36	701.0
37	710.0
38	1129.0
39	1548.0
40	1548.0
41	2257.5
42	2967.0
43	3900.5
44	4834.0
45	7874.0
46	10914.0
47	10914.0
48	12643.5
49	14373.0
50	16424.5
51	18476.0
52	19019.5
53	19563.0
54	19563.0
55	18037.5
56	16512.0
57	16031.5
58	15551.0
59	14756.0
60	13961.0
61	13961.0
62	12754.0
63	11547.0
64	9126.5
65	6706.0
66	5768.5
67	4831.0
68	4831.0
69	4031.0
70	3231.0
71	2691.0
72	2151.0
73	1686.0
74	1221.0
75	1221.0
76	970.5
77	720.0
78	590.5
79	461.0
80	369.0
81	277.0
82	277.0
83	238.5
84	200.0
85	143.0
86	86.0
87	72.5
88	59.0
89	59.0
90	41.5
91	24.0
92	18.5
93	13.0
94	8.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	153080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.24091978050693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.87868407063965	37.10870133263653
2	6.929596597956412	6.131434544029266
3	2.5603921800248064	3.398223151293441
4	1.3791270450652648	2.4405539587143976
5	0.8977615025692517	1.9858897308596812
6	0.5950623117358691	1.5795662398745753
7	0.4429744256098282	1.3718317219754377
8	0.35585612190656196	1.2594721714136399
9	0.2894099580650877	1.1523386464593677
>10	2.2252081979800367	19.60870133263653
>50	0.26578465536589685	8.420433760125425
>100	0.17571318882523182	14.176247713613796
>500	0.004429744256098282	1.3666056963679123
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	911	0.5951136660569636	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	657	0.4291873530180298	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	524	0.34230467729291875	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	417	0.2724065847922655	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	405	0.2645675463809773	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	387	0.25280898876404495	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	377	0.2462764567546381	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	356	0.23255813953488372	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	352	0.22994512673112097	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	350	0.2286386203292396	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	344	0.22471910112359553	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	343	0.22406584792265483	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	338	0.2207995819179514	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	323	0.21100078390384114	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	321	0.20969427750195976	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	321	0.20969427750195976	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	311	0.2031617454925529	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	302	0.19728246668408675	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	296	0.19336294747844265	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	285	0.1861771622680951	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	283	0.18487065586621373	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	272	0.17768487065586622	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	271	0.17703161745492554	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	268	0.17507185785210347	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	260	0.169845832244578	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	252	0.1646198066370525	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	249	0.16266004703423048	Illumina PCR Primer Index 10 (95% over 22bp)
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	248	0.16200679383328978	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	241	0.15743402142670498	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	238	0.15547426182388294	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	236	0.1541677554220016	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	228	0.1489417298144761	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	219	0.14306245100600992	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	214	0.1397961850013065	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	214	0.1397961850013065	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	214	0.1397961850013065	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	206	0.13457015939378103	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	205	0.13391690619284036	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	204	0.13326365299189966	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	192	0.12542461458061144	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	192	0.12542461458061144	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	191	0.12477136137967076	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	185	0.12085184217402667	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	182	0.11889208257120461	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	182	0.11889208257120461	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	182	0.11889208257120461	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	176	0.11497256336556048	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	175	0.1143193101646198	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	174	0.11366605696367911	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	174	0.11366605696367911	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	173	0.11301280376273842	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	171	0.11170629736085708	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	171	0.11170629736085708	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	170	0.11105304415991639	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	168	0.10974653775803501	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	168	0.10974653775803501	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	164	0.10713352495427227	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	162	0.1058270185523909	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	160	0.10452051215050953	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	160	0.10452051215050953	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	159	0.10386725894956886	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	158	0.10321400574862817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.532532009406846E-4
2	0.0	0.0	0.0	0.0	6.532532009406846E-4
3	0.0	0.0	0.0	0.0	6.532532009406846E-4
4	0.0	0.0	0.0	0.0	6.532532009406846E-4
5	0.0	0.0	0.0	0.0	6.532532009406846E-4
6	0.0	0.0	0.0	0.0	6.532532009406846E-4
7	0.0	0.0	0.0	0.0	6.532532009406846E-4
8	0.0	0.0	0.0	0.0	6.532532009406846E-4
9	0.0	0.0	0.0	0.0	6.532532009406846E-4
10	0.0	0.0	0.0	0.0	6.532532009406846E-4
11	0.0	0.0	0.0	0.0	6.532532009406846E-4
12	0.0	0.0	0.0	0.0	6.532532009406846E-4
13	0.0	0.0	0.0	0.0	6.532532009406846E-4
14	0.0	0.0	0.0	0.0	6.532532009406846E-4
15	0.0	0.0	0.0	0.0	6.532532009406846E-4
16	6.532532009406846E-4	0.0	0.0	6.532532009406846E-4	6.532532009406846E-4
17	6.532532009406846E-4	0.0	0.0	0.0026130128037627383	6.532532009406846E-4
18	6.532532009406846E-4	0.0	0.0	0.0026130128037627383	6.532532009406846E-4
19	6.532532009406846E-4	0.0	0.0	0.003266266004703423	6.532532009406846E-4
20	6.532532009406846E-4	0.0	0.0	0.003919519205644107	6.532532009406846E-4
21	0.0013065064018813691	0.0	0.0	0.004572772406584792	6.532532009406846E-4
22	0.0013065064018813691	0.0	0.0	0.011105304415991639	6.532532009406846E-4
23	0.0013065064018813691	0.0	0.0	0.012411810817873008	6.532532009406846E-4
24	0.0013065064018813691	0.0	0.0	0.01567807682257643	6.532532009406846E-4
25	0.0013065064018813691	0.0	0.0	0.0169845832244578	6.532532009406846E-4
26	0.0013065064018813691	0.0	0.0	0.02286386203292396	6.532532009406846E-4
27	0.0013065064018813691	0.0	0.0	0.06271230729030572	6.532532009406846E-4
28	0.0013065064018813691	0.0	0.0	0.20120198588973087	6.532532009406846E-4
29	0.0013065064018813691	0.0	0.0	0.387379148157826	6.532532009406846E-4
30	0.0013065064018813691	0.0	0.0	0.644107656127515	6.532532009406846E-4
31	0.0013065064018813691	0.0	0.0	1.2228899921609615	6.532532009406846E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	230	0.0	31.369566	1
TGGAGAG	25	0.0054816534	29.599998	5
CACGAGA	50	2.6947964E-7	29.599998	11
CTGAAGG	25	0.0054816534	29.599998	4
CTGTTTG	25	0.0054816534	29.599998	9
GCCTATC	50	2.6947964E-7	29.599998	23
TCTCCGA	50	2.6947964E-7	29.599998	1
AGCCTAT	50	2.6947964E-7	29.599998	22
AAAGACT	25	0.0054816534	29.599998	36
TACCGCT	25	0.0054816534	29.599998	35
TATCTCG	60	4.2715328E-8	27.749998	26
ATCTCGT	55	6.2023355E-7	26.90909	27
CTATCTC	55	6.2023355E-7	26.90909	25
TCGTATG	55	6.2023355E-7	26.90909	30
GCCGTCT	55	6.2023355E-7	26.90909	36
CACAGCT	35	8.834167E-4	26.42857	34
GAGCCTA	65	9.2810296E-8	25.615385	21
CTCGTAT	60	1.3247936E-6	24.666666	29
AGCCCAC	60	1.3247936E-6	24.666666	7
TATGCCG	60	1.3247936E-6	24.666666	33
>>END_MODULE