FastQCFastQC Report
Fri 10 Feb 2017
ERR1632517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127132
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3570.28081049617720166No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2660.20923135009281693No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2230.17540823710788786No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1990.15653022055816004No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1860.14630462826039076No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1790.1407985401000535No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1690.1329326998710002No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1560.12270710757323097No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1490.1172010194128937No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1450.11405468332127237No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1400.11012176320674574No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1380.1085485951609351No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1360.10697542711512442No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1350.10618884309221911No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1350.10618884309221911No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1320.10382909102350313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGATT200.001835784537.015
GATTCCT200.001835784537.018
CTATGGG352.3717545E-531.7142873
TCTATGG352.3717545E-531.7142872
GGTATCA1500.029.61
GTGATTC250.005478197229.59999816
AACGACC250.005478197229.59999837
TATACAC509.00831E-625.89999837
TATGGGC451.3141884E-424.6666664
GGGCAGT400.001921803523.1257
CTCTATG400.001921803523.1251
CTTATAC1650.022.42424237
TAATACG1059.586074E-1021.14285727
TGATAGT450.003807395720.55555518
CAGTTAA450.003807395720.55555511
GTGTCGA450.003807395720.55555525
CAGTCGG450.003807395720.55555510
TGAGCAA450.003807395720.55555536
GTATCAA2100.020.2619042
ATGGGCA555.108587E-420.1818185
Produced by FastQC (version 0.11.5)