##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632517.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127132
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82914608438473	34.0	31.0	34.0	31.0	34.0
2	32.984079539376395	34.0	33.0	34.0	31.0	34.0
3	33.06475159676557	34.0	33.0	34.0	31.0	34.0
4	36.440054431614385	37.0	37.0	37.0	35.0	37.0
5	36.37206997451468	37.0	37.0	37.0	35.0	37.0
6	36.40208602082875	37.0	37.0	37.0	35.0	37.0
7	36.39923858666583	37.0	37.0	37.0	35.0	37.0
8	36.36722461693358	37.0	37.0	37.0	35.0	37.0
9	38.1070226221565	39.0	38.0	39.0	37.0	39.0
10	38.11624925274518	39.0	38.0	39.0	37.0	39.0
11	38.19432558285876	39.0	38.0	39.0	37.0	39.0
12	38.134466538715664	39.0	38.0	39.0	37.0	39.0
13	38.19807758864802	39.0	38.0	39.0	37.0	39.0
14	39.67466884812636	41.0	40.0	41.0	38.0	41.0
15	39.66114746877261	41.0	40.0	41.0	38.0	41.0
16	39.6347733064846	41.0	40.0	41.0	37.0	41.0
17	39.62136204889406	41.0	40.0	41.0	37.0	41.0
18	39.61422773180631	41.0	40.0	41.0	37.0	41.0
19	39.60992511720102	41.0	40.0	41.0	37.0	41.0
20	39.589820029575556	41.0	40.0	41.0	37.0	41.0
21	39.54983009785105	41.0	39.0	41.0	37.0	41.0
22	39.51983764905767	41.0	39.0	41.0	37.0	41.0
23	39.47962747380675	41.0	39.0	41.0	37.0	41.0
24	39.4473696630274	41.0	39.0	41.0	37.0	41.0
25	39.41126545637605	40.0	39.0	41.0	37.0	41.0
26	39.328320171160684	40.0	39.0	41.0	36.0	41.0
27	39.210458421168546	40.0	39.0	41.0	36.0	41.0
28	39.166464776767455	40.0	39.0	41.0	36.0	41.0
29	39.103254884686784	40.0	39.0	41.0	36.0	41.0
30	39.06940030834094	40.0	39.0	41.0	35.0	41.0
31	39.0051442595098	40.0	39.0	41.0	35.0	41.0
32	38.949170940439856	40.0	39.0	41.0	35.0	41.0
33	38.85594500204512	40.0	38.0	41.0	35.0	41.0
34	38.8178743353365	40.0	38.0	41.0	35.0	41.0
35	38.74905609917251	40.0	38.0	41.0	35.0	41.0
36	38.689991504892554	40.0	38.0	41.0	35.0	41.0
37	38.63403391750307	40.0	38.0	41.0	35.0	41.0
38	38.552465154327784	40.0	38.0	41.0	35.0	41.0
39	38.46305414844414	40.0	38.0	41.0	35.0	41.0
40	38.37807947644968	40.0	38.0	41.0	34.0	41.0
41	38.31017367775226	40.0	38.0	41.0	34.0	41.0
42	38.23645502312557	40.0	37.0	41.0	34.0	41.0
43	37.45770537708838	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	10.0
21	21.0
22	32.0
23	57.0
24	68.0
25	141.0
26	184.0
27	249.0
28	387.0
29	506.0
30	680.0
31	941.0
32	1204.0
33	1714.0
34	2516.0
35	3951.0
36	6567.0
37	12603.0
38	34035.0
39	61261.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.363590598747756	15.9920397696882	12.213290123651008	28.431079507913037
2	22.124248812258127	18.79149230720826	30.893087499606708	28.19117138092691
3	21.877261429065854	18.99443098511783	27.92451939716201	31.203788188654315
4	16.52062423308058	15.120504672309096	32.59132240505931	35.76754868955101
5	18.056036245791777	32.12015857533902	32.416700751974325	17.407104426894882
6	36.357486706730015	32.33096309347764	14.427524148129503	16.88402605166284
7	31.370544001510243	26.436302425825126	18.929144511216688	23.264009061447943
8	28.08498253783469	29.975143944876194	19.56627756977	22.373595947519114
9	28.015763143819022	12.424881225812541	16.7455872636315	42.81376836673694
10	19.811691784916466	22.844759777239403	29.117767359909386	28.225781077934748
11	40.709656105465186	18.58068778906963	18.268413931976212	22.441242173488973
12	23.568417078312308	22.65519302771922	26.344272095145204	27.43211779882327
13	36.17657238146179	16.873800459365068	19.65909448447283	27.290532674700312
14	24.60434823647862	18.774974042727244	22.102224459616775	34.518453261177356
15	30.16235094232766	23.111411761004312	18.882736053865273	27.843501242802756
16	27.048264795645473	22.64496743542145	21.671176415064657	28.635591353868424
17	27.96699493439889	22.521473743825315	21.21181134568795	28.299719976087843
18	27.544599314098733	20.737501179876034	22.405059308435327	29.312840197589907
19	28.843249535915426	21.83714564389768	22.23122423937325	27.08838058081364
20	30.001887801654973	20.547934430355852	21.635780134033915	27.814397633955256
21	28.265896863102917	20.94043985778561	21.44857313658245	29.345090142529024
22	29.108328351634523	21.72466412862222	20.63839159298996	28.528615926753297
23	28.900670169587517	21.306201428436587	21.437560960261777	28.355567441714125
24	28.60176824088349	20.88144605606771	21.638139886102632	28.878645816946165
25	29.353742566780983	21.166976056382342	21.678255671270804	27.80102570556587
26	28.95337129912217	21.63892647012554	21.54610955542271	27.86159267532958
27	27.909574300726803	21.417109775666237	22.78025988736117	27.893056036245788
28	27.38334958940314	22.258754680174935	21.304628260390775	29.053267470031148
29	27.98901928704024	22.688229556681243	21.374634238429348	27.948116917849163
30	27.926092565207817	21.240914954535445	22.750369694490765	28.082622785765977
31	28.673347386967873	21.73803605701161	21.59723751691156	27.99137903910896
32	26.981405153698518	21.319573356825977	22.323254570053173	29.37576691942233
33	27.034892867256083	21.18900040902369	23.253783469150175	28.522323254570054
34	27.438410471006513	21.071012805587895	23.073655727904853	28.41692099550074
35	26.696661737406792	22.1942547902967	23.578642670610076	27.530440801686435
36	27.024667274958308	20.868860711701224	24.351068181103106	27.755403832237356
37	27.255922977692475	20.225434980964664	24.082843029292388	28.435799012050467
38	26.17672969826637	19.950130572947803	25.643425730736556	28.22971399804927
39	25.66623666740081	19.312997514394485	26.201113802976433	28.819652015228264
40	25.409810275933676	19.942264732718748	27.603593115816633	27.044331875530947
41	23.91923355252808	19.298052417959287	28.181732372652046	28.600981656860586
42	22.342919170625805	20.212063052575278	29.482742346537457	27.962275430261464
43	21.658591070698172	20.555013686562	28.870779976717113	28.915615266022716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	2.0
6	4.5
7	7.0
8	7.5
9	8.0
10	8.5
11	9.0
12	9.0
13	16.0
14	23.0
15	48.0
16	73.0
17	69.0
18	65.0
19	65.0
20	80.0
21	95.0
22	94.5
23	94.0
24	109.5
25	125.0
26	125.0
27	175.5
28	226.0
29	306.0
30	386.0
31	520.0
32	654.0
33	654.0
34	874.5
35	1095.0
36	1285.5
37	1476.0
38	1958.5
39	2441.0
40	2441.0
41	2982.5
42	3524.0
43	4185.0
44	4846.0
45	6369.0
46	7892.0
47	7892.0
48	8957.5
49	10023.0
50	11240.0
51	12457.0
52	12999.0
53	13541.0
54	13541.0
55	13129.5
56	12718.0
57	12472.0
58	12226.0
59	11775.5
60	11325.0
61	11325.0
62	10563.5
63	9802.0
64	8379.0
65	6956.0
66	6092.0
67	5228.0
68	5228.0
69	4379.0
70	3530.0
71	3039.5
72	2549.0
73	2052.5
74	1556.0
75	1556.0
76	1235.0
77	914.0
78	743.0
79	572.0
80	453.0
81	334.0
82	334.0
83	262.5
84	191.0
85	141.5
86	92.0
87	71.0
88	50.0
89	50.0
90	33.0
91	16.0
92	11.5
93	7.0
94	4.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	127132.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.22543498096466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.50840623214866	63.55992197086493
2	5.042619204964213	7.082402542239562
3	1.4471488255916845	3.0487996727810462
4	0.7594171081665342	2.1332158701192463
5	0.4480336921336484	1.5731680458106534
6	0.28898173142620326	1.217632067457446
7	0.221776677606156	1.0902054557467828
8	0.17137288724112054	0.9627788440361199
9	0.12992977071875805	0.8211937199131611
>10	0.8815062892729533	11.753138470251393
>50	0.06832513805038139	3.35792719378284
>100	0.03248244267968951	3.3996161469968222
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	357	0.28081049617720166	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	266	0.20923135009281693	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	223	0.17540823710788786	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	199	0.15653022055816004	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	186	0.14630462826039076	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	179	0.1407985401000535	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	169	0.1329326998710002	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	156	0.12270710757323097	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	149	0.1172010194128937	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	145	0.11405468332127237	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	140	0.11012176320674574	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	138	0.1085485951609351	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	136	0.10697542711512442	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	135	0.10618884309221911	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	135	0.10618884309221911	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	132	0.10382909102350313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0015731680458106535	0.0
22	0.0	0.0	0.0	0.003932920114526633	0.0
23	0.0	0.0	0.0	0.005506088160337287	0.0
24	0.0	0.0	0.0	0.007079256206147941	0.0
25	0.0	0.0	0.0	0.008652424251958593	0.0
26	0.0	0.0	0.0	0.0117987603435799	0.0
27	0.0	0.0	0.0	0.03775603309945568	0.0
28	0.0	0.0	0.0	0.14237170814586414	0.0
29	0.0	0.0	0.0	0.3091275210017934	0.0
30	0.0	0.0	0.0	0.5340905515527169	0.0
31	0.0	0.0	0.0	1.1311078249378599	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGATT	20	0.0018357845	37.0	15
GATTCCT	20	0.0018357845	37.0	18
CTATGGG	35	2.3717545E-5	31.714287	3
TCTATGG	35	2.3717545E-5	31.714287	2
GGTATCA	150	0.0	29.6	1
GTGATTC	25	0.0054781972	29.599998	16
AACGACC	25	0.0054781972	29.599998	37
TATACAC	50	9.00831E-6	25.899998	37
TATGGGC	45	1.3141884E-4	24.666666	4
GGGCAGT	40	0.0019218035	23.125	7
CTCTATG	40	0.0019218035	23.125	1
CTTATAC	165	0.0	22.424242	37
TAATACG	105	9.586074E-10	21.142857	27
TGATAGT	45	0.0038073957	20.555555	18
CAGTTAA	45	0.0038073957	20.555555	11
GTGTCGA	45	0.0038073957	20.555555	25
CAGTCGG	45	0.0038073957	20.555555	10
TGAGCAA	45	0.0038073957	20.555555	36
GTATCAA	210	0.0	20.261904	2
ATGGGCA	55	5.108587E-4	20.181818	5
>>END_MODULE