FastQCFastQC Report
Fri 10 Feb 2017
ERR1632516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20492
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1570.7661526449346087No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1290.629513956666016TruSeq Adapter, Index 12 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA920.4489556900253757No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA890.4343158305680266No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA600.2927971891469842No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT600.2927971891469842No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT570.27815732968963497No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC570.27815732968963497No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC550.2683974233847355No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA520.2537575639273863No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA520.2537575639273863No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT510.24887761077493656No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA490.2391177044700371No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC480.23423775131758734No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA470.22935779816513763No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC460.22447784501268786No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC460.22447784501268786No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA460.22447784501268786No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT450.21959789186023815No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC450.21959789186023815No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA450.21959789186023815No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA450.21959789186023815No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC430.2098379855553387No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG420.20495803240288893No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT410.20007807925043922No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC410.20007807925043922No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT400.19519812609798945No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA400.19519812609798945No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG380.18543821979309No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA380.18543821979309No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG360.17567831348819052No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC350.17079836033574078No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC350.17079836033574078No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC350.17079836033574078No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT350.17079836033574078No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC340.16591840718329104No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT340.16591840718329104No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC340.16591840718329104No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC340.16591840718329104No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG340.16591840718329104No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC330.1610384540308413No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC330.1610384540308413RNA PCR Primer, Index 12 (95% over 23bp)
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT310.15127854772594185No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC290.14151864142104234No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC290.14151864142104234No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC280.1366386882685926No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG280.1366386882685926No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA270.1317587351161429No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG270.1317587351161429No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA260.12687878196369315No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA260.12687878196369315No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT250.12199882881124341No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG250.12199882881124341No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA250.12199882881124341No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA240.11711887565879367No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA240.11711887565879367No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC240.11711887565879367No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG240.11711887565879367No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG240.11711887565879367No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA240.11711887565879367No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC230.11223892250634393No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC230.11223892250634393No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT230.11223892250634393No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA230.11223892250634393No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG220.1073589693538942No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG220.1073589693538942No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC220.1073589693538942No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA220.1073589693538942No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG220.1073589693538942No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC210.10247901620144446No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA210.10247901620144446No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG210.10247901620144446No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA210.10247901620144446No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC210.10247901620144446No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC210.10247901620144446No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC210.10247901620144446No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA210.10247901620144446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG500.006806995718.49999827
CTGTCTC1900.00212748338.76315837
Produced by FastQC (version 0.11.5)