##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632516.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20492
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97135467499512	34.0	33.0	34.0	31.0	34.0
2	33.10135662697638	34.0	33.0	34.0	31.0	34.0
3	33.19119656451298	34.0	33.0	34.0	31.0	34.0
4	36.46759711106773	37.0	37.0	37.0	35.0	37.0
5	36.43826859262151	37.0	37.0	37.0	35.0	37.0
6	36.49058169041577	37.0	37.0	37.0	35.0	37.0
7	36.49565684169432	37.0	37.0	37.0	35.0	37.0
8	36.50009759906305	37.0	37.0	37.0	35.0	37.0
9	38.25502635174702	39.0	39.0	39.0	37.0	39.0
10	38.278157329689634	39.0	39.0	39.0	37.0	39.0
11	38.35482139371462	39.0	39.0	39.0	37.0	39.0
12	38.31934413429631	39.0	39.0	39.0	37.0	39.0
13	38.34037673238337	39.0	39.0	39.0	37.0	39.0
14	39.86092133515518	41.0	40.0	41.0	38.0	41.0
15	39.844622291626	41.0	40.0	41.0	38.0	41.0
16	39.779426117509274	41.0	40.0	41.0	38.0	41.0
17	39.81690415772009	41.0	40.0	41.0	38.0	41.0
18	39.81226820222526	41.0	40.0	41.0	38.0	41.0
19	39.80162990435292	41.0	40.0	41.0	38.0	41.0
20	39.8129513956666	41.0	40.0	41.0	38.0	41.0
21	39.77113019715011	41.0	40.0	41.0	38.0	41.0
22	39.70466523521374	41.0	40.0	41.0	38.0	41.0
23	39.64883857114972	41.0	40.0	41.0	37.0	41.0
24	39.6262931875854	41.0	40.0	41.0	37.0	41.0
25	39.62697638102674	41.0	40.0	41.0	37.0	41.0
26	39.49868241264884	41.0	39.0	41.0	37.0	41.0
27	39.324175287917235	41.0	39.0	41.0	36.0	41.0
28	39.308071442514155	41.0	39.0	41.0	36.0	41.0
29	39.29416357602967	41.0	39.0	41.0	36.0	41.0
30	39.28108530158111	41.0	39.0	41.0	36.0	41.0
31	39.162404840913524	41.0	39.0	41.0	35.0	41.0
32	39.1286843646301	41.0	39.0	41.0	35.0	41.0
33	39.043968377903575	40.0	39.0	41.0	35.0	41.0
34	39.00961350771033	40.0	39.0	41.0	35.0	41.0
35	38.927581495217645	40.0	39.0	41.0	35.0	41.0
36	38.843060706617216	40.0	38.0	41.0	35.0	41.0
37	38.74960960374781	40.0	38.0	41.0	35.0	41.0
38	38.621510833496	40.0	38.0	41.0	35.0	41.0
39	38.45783720476283	40.0	38.0	41.0	35.0	41.0
40	38.38102674214328	40.0	37.0	41.0	35.0	41.0
41	38.31339059145032	40.0	37.0	41.0	35.0	41.0
42	38.22223306656256	40.0	37.0	41.0	34.0	41.0
43	37.455592426312705	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0
22	4.0
23	11.0
24	8.0
25	23.0
26	34.0
27	46.0
28	56.0
29	89.0
30	85.0
31	146.0
32	184.0
33	252.0
34	387.0
35	582.0
36	916.0
37	1712.0
38	4669.0
39	11285.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.87526839742338	15.440171774350967	12.643958617997267	29.040601210228385
2	22.706422018348622	20.110286941245363	29.582276010150306	27.60101503025571
3	22.725941830958423	17.96798750731993	28.484286550849113	30.821784110872535
4	15.708569197735702	16.51376146788991	34.85750536794846	32.920163966425925
5	18.109506148740973	31.44153816123365	31.631856334179194	18.817099355846185
6	32.00273277376537	33.798555533866875	16.27464376341987	17.92406792894788
7	29.162600039039628	26.659184071832907	18.50966230724185	25.66855358188561
8	26.693343743900062	28.752683974233843	21.2717157915284	23.282256490337694
9	26.99102088619949	12.370681241460083	16.987116923677533	43.65118094866289
10	21.008198321296113	21.06675775912551	27.04470037087644	30.880343548701934
11	40.898887370681244	18.446222916260005	18.050946710911575	22.60394300214718
12	21.65235213741948	22.843060706617216	26.11262931875854	29.391957837204764
13	38.89810657817685	16.416162404840914	18.50966230724185	26.176068709740385
14	24.42904548116338	19.45149326566465	22.07202810853016	34.04743314464181
15	31.539137224282648	22.359945344524693	18.34374390005856	27.7571735311341
16	25.30255709545188	22.95529962912356	22.2525863751708	29.489556900253756
17	27.7571735311341	22.940659769666212	20.52996291235604	28.772203786843647
18	27.508295920359167	19.09037673238337	22.02810853015811	31.373218817099357
19	29.601795822760103	21.593792699590082	22.1842670310365	26.620144446613313
20	31.792894788210035	18.93909818465743	20.329884833105602	28.93812219402694
21	29.28459886785087	20.022447784501267	20.68612141323443	30.00683193441343
22	29.343158305680266	21.735311341011126	19.822369705250832	29.099160648057783
23	29.836033574077685	20.80812024204568	20.876439586179973	28.47940659769666
24	29.61643568221745	19.070856919773572	21.676751903181728	29.63595549482725
25	30.18251024790162	21.676751903181728	21.42787429240679	26.71286355650986
26	30.602186219012296	21.769471013078274	20.212765957446805	27.41557681046262
27	27.55709545188366	20.827640054655475	24.336326371266836	27.27893812219403
28	27.303337887956275	23.1358578957642	20.17372633222721	29.387077884052314
29	28.176849502244778	23.414015225453834	20.212765957446805	28.196369314854575
30	27.43509662307242	20.73004099160648	24.41440562170603	27.42045676361507
31	29.069880929143082	21.68651180948663	21.149716962717157	28.093890298653136
32	26.883661916845597	20.52508295920359	21.87195002927972	30.71930509467109
33	26.917821588912744	20.53484286550849	23.52625414796018	29.02108139761858
34	27.049580324028888	21.452274058169042	21.86219012297482	29.63595549482725
35	26.18582861604529	22.994339254343156	24.02400936951005	26.7958227601015
36	27.91821198516494	21.447394105016592	23.653132929923874	26.981260979894593
37	29.191879757954325	20.461643568221746	23.043138785867654	27.303337887956275
38	27.332617606870972	19.505172750341597	25.048799531524494	28.113410111262933
39	27.147179387077884	18.841499121608432	25.453835643177825	28.55748584813586
40	25.81983212961156	20.320124926800702	27.53269568612141	26.32734725746633
41	24.185047823540895	19.729650595354283	27.069100136638692	29.016201444466134
42	21.418114386101895	20.237165723209056	30.670505563146595	27.674214327542458
43	21.369314854577397	21.989068904938513	27.332617606870972	29.308998633613115
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.5
9	1.0
10	2.5
11	4.0
12	4.0
13	7.5
14	11.0
15	14.5
16	18.0
17	15.0
18	12.0
19	12.0
20	16.5
21	21.0
22	15.0
23	9.0
24	9.0
25	9.0
26	9.0
27	9.5
28	10.0
29	12.0
30	14.0
31	12.5
32	11.0
33	11.0
34	32.0
35	53.0
36	44.5
37	36.0
38	87.0
39	138.0
40	138.0
41	241.5
42	345.0
43	456.0
44	567.0
45	951.0
46	1335.0
47	1335.0
48	1601.0
49	1867.0
50	2143.0
51	2419.0
52	2511.5
53	2604.0
54	2604.0
55	2476.5
56	2349.0
57	2270.0
58	2191.0
59	2014.5
60	1838.0
61	1838.0
62	1744.5
63	1651.0
64	1297.0
65	943.0
66	857.0
67	771.0
68	771.0
69	643.0
70	515.0
71	416.0
72	317.0
73	252.5
74	188.0
75	188.0
76	152.5
77	117.0
78	95.5
79	74.0
80	54.0
81	34.0
82	34.0
83	23.5
84	13.0
85	9.0
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	20492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38746828030451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.20264317180617	45.52996291235604
2	7.691629955947136	8.52039820417724
3	3.101321585903084	5.153230528986922
4	1.7004405286343611	3.7673238336911967
5	1.1013215859030838	3.049970720281085
6	0.7312775330396476	2.430216669919969
7	0.5286343612334802	2.0495803240288892
8	0.46696035242290745	2.0691001366386885
9	0.30837004405286345	1.537185243021667
>10	2.0616740088105727	21.447394105016592
>50	0.0881057268722467	3.049970720281085
>100	0.01762114537444934	1.3956666016006247
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	157	0.7661526449346087	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	129	0.629513956666016	TruSeq Adapter, Index 12 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	92	0.4489556900253757	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	89	0.4343158305680266	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	60	0.2927971891469842	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	60	0.2927971891469842	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	57	0.27815732968963497	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	57	0.27815732968963497	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	55	0.2683974233847355	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	52	0.2537575639273863	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	52	0.2537575639273863	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	51	0.24887761077493656	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	49	0.2391177044700371	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	48	0.23423775131758734	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	47	0.22935779816513763	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	46	0.22447784501268786	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	46	0.22447784501268786	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	46	0.22447784501268786	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	45	0.21959789186023815	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	45	0.21959789186023815	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	45	0.21959789186023815	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	45	0.21959789186023815	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	43	0.2098379855553387	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	42	0.20495803240288893	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	41	0.20007807925043922	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	41	0.20007807925043922	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	40	0.19519812609798945	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	40	0.19519812609798945	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	38	0.18543821979309	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	38	0.18543821979309	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	36	0.17567831348819052	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	35	0.17079836033574078	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	35	0.17079836033574078	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	35	0.17079836033574078	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	35	0.17079836033574078	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	34	0.16591840718329104	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	34	0.16591840718329104	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	34	0.16591840718329104	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	34	0.16591840718329104	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	34	0.16591840718329104	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	33	0.1610384540308413	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	33	0.1610384540308413	RNA PCR Primer, Index 12 (95% over 23bp)
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	31	0.15127854772594185	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	29	0.14151864142104234	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	29	0.14151864142104234	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	28	0.1366386882685926	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	28	0.1366386882685926	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	27	0.1317587351161429	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	27	0.1317587351161429	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	26	0.12687878196369315	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	26	0.12687878196369315	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	25	0.12199882881124341	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	25	0.12199882881124341	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	25	0.12199882881124341	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	24	0.11711887565879367	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	24	0.11711887565879367	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	24	0.11711887565879367	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	24	0.11711887565879367	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	24	0.11711887565879367	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	24	0.11711887565879367	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	23	0.11223892250634393	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	23	0.11223892250634393	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	23	0.11223892250634393	No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA	23	0.11223892250634393	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	22	0.1073589693538942	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	22	0.1073589693538942	No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC	22	0.1073589693538942	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	22	0.1073589693538942	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	22	0.1073589693538942	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	21	0.10247901620144446	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	21	0.10247901620144446	No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG	21	0.10247901620144446	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	21	0.10247901620144446	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	21	0.10247901620144446	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	21	0.10247901620144446	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	21	0.10247901620144446	No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA	21	0.10247901620144446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.004879953152449736	0.0
26	0.0	0.0	0.0	0.014639859457349209	0.0
27	0.0	0.0	0.0	0.03415967206714816	0.0
28	0.0	0.0	0.0	0.15615850087839156	0.0
29	0.0	0.0	0.0	0.28303728284208474	0.0
30	0.0	0.0	0.0	0.4831153620925239	0.0
31	0.0	0.0	0.0	0.888151473745852	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	50	0.0068069957	18.499998	27
CTGTCTC	190	0.0021274833	8.763158	37
>>END_MODULE