FastQCFastQC Report
Fri 10 Feb 2017
ERR1632515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330730
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20890.6316330541529345No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15730.4756145496326309No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12430.37583527348592505No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10130.30629214162609986No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT9650.2917787923683972No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9630.2911740694826596No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9610.29056934659692196No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT9520.28784809361110275No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8810.26638043116741755No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC8210.24823874459528922No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8080.24430804583799476No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8070.24400568439512596No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7740.23402775678045534No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7720.23342303389471772No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7720.23342303389471772No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7590.22949233513742326No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7380.22314274483717836No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC7220.21830496175127748No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6970.21074592567955736No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6930.2095364799080821No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6680.201977443836362No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6380.19290660055029782No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6310.19079007045021618No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6280.18988298612160975No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6200.1874640945786593No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5940.1796026970640704No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5930.1793003356212016No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5930.1793003356212016No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5620.1699271308922686No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC5480.16569407069210534No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5350.16176337193481086No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5170.15632086596317238No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5160.15601850452030358No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5160.15601850452030358No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4960.14997127566292748No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA4920.14876182989145226No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4920.14876182989145226No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4860.1469476612342394No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4860.1469476612342394No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4800.1451334925770266No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4670.1412027938197321No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4490.1357602878480936No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4490.1357602878480936No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4490.1357602878480936No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4360.13182958909079914No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4310.13031778187645512No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4300.1300154204335863No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4290.1297130589907175No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4290.1297130589907175No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT4220.12759652889063586RNA PCR Primer, Index 17 (95% over 21bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4200.12699180600489826No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4120.12457291446194782No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4050.12245638436186618No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4020.12154930003325977No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG3940.11913040849030933No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3920.11852568560457172No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA3920.11852568560457172No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3850.11640915550449006No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3680.11126901097572037No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC3660.11066428808998276No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3640.11005956520424515No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3560.1076406736612947No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3470.10491942067547547No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3460.10461705923260665No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3440.10401233634686904No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC3430.10370997490400025No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3420.10340761346113143No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3420.10340761346113143No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC3410.10310525201826264No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3390.10250052913252503No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3390.10250052913252503No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC3370.10189580624678742No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3340.100988721918181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGA451.076005E-732.888895
CTTTTTA451.076005E-732.888892
TTTTTAC451.076005E-732.888893
GCTTTTT451.076005E-732.888891
CCGCTTA303.5939846E-430.83333425
GCTTATT303.5939846E-430.83333427
GGAATTT250.005490753329.621
GTAGGCT250.005490753329.611
CGTGTAG250.005490753329.68
AACAGGC250.005490753329.629
TTTTACG502.7108763E-729.64
GGTATCA6600.029.1515141
TCAATGC453.9969163E-628.77777732
CAATGCT453.9969163E-628.77777733
TTATTGA905.456968E-1226.72222129
AGTCGGT905.456968E-1226.72222111
ATTGATA706.555638E-926.42857231
ATACGGC1600.025.43750229
CTTATTG951.0913936E-1125.3157928
GGTGATT951.0913936E-1125.3157915
Produced by FastQC (version 0.11.5)