FastQCFastQC Report
Fri 10 Feb 2017
ERR1632514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237704
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5720.2406354121091778No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4280.1800558678019722No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3670.15439369972739203No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3360.14135227005014642No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3080.1295729142126342No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2840.11947632349476661No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2750.11569010197556626No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2640.1110624978965436No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2390.10054521589876486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAATG303.5909703E-430.8333322
CATGCGG250.00548768629.59999810
TCTTAGA250.00548768629.5999982
AGGTGAC250.00548768629.59999830
GGTATCA3100.027.4516121
TTGTAAT358.848629E-426.428571
GTAATGC358.848629E-426.428573
ATACGGC509.0515405E-625.89999829
ATACACA601.329965E-624.66666637
GTCTTGG551.8944405E-523.5454541
GGCATAT400.001926708523.1258
TTAGCCC1200.023.12499829
AGTGGTT502.693375E-422.19999927
CGCGGTA2400.021.58333222
GCGGTAA2350.021.25531823
TAATGCT450.003817020420.5555554
GTATCAA4150.020.5060232
ACGACCA555.126618E-420.1818185
TAATACG2150.019.79069727
CGTGCCA2550.019.58823610
Produced by FastQC (version 0.11.5)