##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632514.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237704
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.037159660754554	34.0	33.0	34.0	31.0	34.0
2	33.179702487126846	34.0	33.0	34.0	31.0	34.0
3	33.25208662874836	34.0	34.0	34.0	31.0	34.0
4	36.54488775956652	37.0	37.0	37.0	35.0	37.0
5	36.51125349173763	37.0	37.0	37.0	35.0	37.0
6	36.55518207518595	37.0	37.0	37.0	35.0	37.0
7	36.54912412075522	37.0	37.0	37.0	35.0	37.0
8	36.528274660922825	37.0	37.0	37.0	35.0	37.0
9	38.32731464342207	39.0	39.0	39.0	37.0	39.0
10	38.34414650153131	39.0	39.0	39.0	37.0	39.0
11	38.415079258237135	39.0	39.0	39.0	37.0	39.0
12	38.37647662639249	39.0	39.0	39.0	37.0	39.0
13	38.41557988086023	39.0	39.0	39.0	37.0	39.0
14	39.97069464544139	41.0	40.0	41.0	38.0	41.0
15	39.960147915054016	41.0	40.0	41.0	38.0	41.0
16	39.927775721064855	41.0	40.0	41.0	38.0	41.0
17	39.918659341029176	41.0	40.0	41.0	38.0	41.0
18	39.918735065459565	41.0	40.0	41.0	38.0	41.0
19	39.91894541109952	41.0	40.0	41.0	38.0	41.0
20	39.89462945512066	41.0	40.0	41.0	38.0	41.0
21	39.8669816242049	41.0	40.0	41.0	38.0	41.0
22	39.83708730185441	41.0	40.0	41.0	38.0	41.0
23	39.79881701612089	41.0	40.0	41.0	38.0	41.0
24	39.78161074277252	41.0	40.0	41.0	38.0	41.0
25	39.739192441019085	41.0	40.0	41.0	38.0	41.0
26	39.65313162588766	41.0	40.0	41.0	37.0	41.0
27	39.56362534917376	41.0	40.0	41.0	37.0	41.0
28	39.51742923972672	41.0	40.0	41.0	37.0	41.0
29	39.48235200080773	41.0	40.0	41.0	37.0	41.0
30	39.43999259583347	41.0	39.0	41.0	37.0	41.0
31	39.3726104735301	41.0	39.0	41.0	36.0	41.0
32	39.323650422374044	41.0	39.0	41.0	36.0	41.0
33	39.25358008279204	41.0	39.0	41.0	36.0	41.0
34	39.21376165314845	41.0	39.0	41.0	35.0	41.0
35	39.14470517955104	41.0	39.0	41.0	35.0	41.0
36	39.09557685188302	41.0	39.0	41.0	35.0	41.0
37	39.042388853363846	41.0	39.0	41.0	35.0	41.0
38	38.96567159155925	40.0	39.0	41.0	35.0	41.0
39	38.86679231312893	40.0	39.0	41.0	35.0	41.0
40	38.79839211792818	40.0	38.0	41.0	35.0	41.0
41	38.752961666610574	40.0	38.0	41.0	35.0	41.0
42	38.69154915356914	40.0	38.0	41.0	35.0	41.0
43	37.96145626493454	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	2.0
18	3.0
19	5.0
20	20.0
21	30.0
22	52.0
23	97.0
24	123.0
25	217.0
26	309.0
27	371.0
28	575.0
29	714.0
30	996.0
31	1341.0
32	1835.0
33	2406.0
34	3570.0
35	5656.0
36	9424.0
37	17774.0
38	46645.0
39	145535.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.492747282334335	15.90170968936156	12.32036482347794	28.285178204826174
2	21.603338605997376	19.211287988422576	31.54679769797732	27.63857570760273
3	21.37069632820651	19.496516676202337	28.28181267458688	30.850974321004276
4	16.309359539595462	15.057382290579882	32.80676808131121	35.826490088513445
5	17.40904654528321	32.91320297512873	32.711271160771375	16.96647931881668
6	36.158836199643254	32.655739911823105	14.645104836266952	16.540319052266685
7	31.268300070676137	26.85146232288897	19.343385050314676	22.536852556120216
8	27.926328543061956	30.595614714098208	19.69971056439942	21.778346178440415
9	28.042439336317436	12.556793322788003	16.910106687308584	42.49066065358597
10	19.578551475785012	23.27726920876384	29.379816915154983	27.764362400296168
11	40.10323764009019	18.89450745464948	18.395567596674855	22.606687308585467
12	23.535152963349375	22.910426412681318	25.88934136573217	27.665079258237135
13	35.54630969609262	17.01443812472655	20.414465048968463	27.024787130212363
14	24.794281964123446	18.620637431427323	22.064416248779995	34.52066435566923
15	29.76937704035271	23.738767542826373	19.27186753272978	27.21998788409114
16	26.891427994480534	23.140544542792718	21.6155386531148	28.352488809611952
17	27.79801770268906	22.88981253996567	21.390468818362333	27.921700938982934
18	27.19474640729647	20.86208057079393	22.75434994783428	29.18882307407532
19	29.14086426816545	21.64078012990947	22.31472756032713	26.903628041597955
20	30.03104701645744	20.60966580284724	21.97144347591963	27.387843704775687
21	28.12826035741931	21.54949012216875	21.267206273348364	29.055043247063573
22	28.93220139332952	21.975650388718744	20.668983273314712	28.423164944637026
23	28.55189647628984	21.774559956921212	21.602917914717466	28.070625652071485
24	28.694931511459632	21.145205802174132	21.607124827516575	28.552737858849664
25	29.13707804664625	21.538552148891057	21.76993235284219	27.5544374516205
26	28.730269578972166	21.841449870427084	21.55495910880759	27.873321441793152
27	27.872059367953423	21.433379328913272	22.726584323360147	27.967976979773162
28	27.15183589674553	22.411486554706695	21.577676437922793	28.859001110624977
29	27.607865244169215	22.913791942920607	21.760256453404235	27.71808635950594
30	27.467775047958803	22.023188503348702	22.883081479487092	27.625954969205395
31	28.259936728031505	21.915491535691448	22.081664591256352	27.742907145020695
32	26.17919765759095	21.588614411200485	22.79052939790664	29.441658533301922
33	26.780786187863896	21.391310200922156	23.359304008346516	28.468599602867435
34	27.01553192205432	21.332834113014506	23.51958738599266	28.132046578938514
35	26.3470534782755	22.477114394372833	23.860768014000605	27.315064113351056
36	26.735772220913407	21.05307441187359	24.60833641840272	27.602816948810283
37	26.89521421599973	20.469154915356917	24.449315114596303	28.186315754047047
38	25.592333322114904	20.041732574967185	26.193501161107935	28.172432941809983
39	25.016406959916537	19.314778043280718	27.14426345370713	28.524551543095615
40	24.72402652037829	19.74009692727089	28.538855046612593	26.99702150573823
41	23.140965234072628	19.50493050180056	29.03232591794837	28.32177834617844
42	21.93189849560798	19.645441389290884	30.123178406757983	28.299481708343148
43	20.908356611584157	19.982835795779625	30.28388247568404	28.824925116952176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.5
4	2.0
5	2.0
6	3.0
7	4.0
8	5.5
9	7.0
10	10.5
11	14.0
12	14.0
13	22.0
14	30.0
15	46.0
16	62.0
17	73.5
18	85.0
19	85.0
20	103.5
21	122.0
22	146.5
23	171.0
24	229.5
25	288.0
26	288.0
27	369.5
28	451.0
29	706.5
30	962.0
31	1197.5
32	1433.0
33	1433.0
34	1884.5
35	2336.0
36	2753.5
37	3171.0
38	4298.0
39	5425.0
40	5425.0
41	6562.5
42	7700.0
43	8704.0
44	9708.0
45	12418.0
46	15128.0
47	15128.0
48	17279.5
49	19431.0
50	21782.5
51	24134.0
52	25674.0
53	27214.0
54	27214.0
55	25288.5
56	23363.0
57	22577.5
58	21792.0
59	20613.5
60	19435.0
61	19435.0
62	18019.5
63	16604.0
64	14234.5
65	11865.0
66	10396.5
67	8928.0
68	8928.0
69	7655.0
70	6382.0
71	5465.5
72	4549.0
73	3710.5
74	2872.0
75	2872.0
76	2293.0
77	1714.0
78	1403.0
79	1092.0
80	847.0
81	602.0
82	602.0
83	458.5
84	315.0
85	250.5
86	186.0
87	128.5
88	71.0
89	71.0
90	50.0
91	29.0
92	21.5
93	14.0
94	10.0
95	6.0
96	6.0
97	5.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	237704.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.2545492074502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.5606578672076	56.01864490905444
2	5.697631270564579	7.208021951396683
3	1.7800632078013678	3.377912873107298
4	0.9279172656693742	2.3477995336690425
5	0.5515428031686325	1.7443795691522637
6	0.4042330620948857	1.5341748070535528
7	0.30287465285909815	1.3410739743075653
8	0.24402987441137455	1.2348799759233744
9	0.16731920004152737	0.9525330515140195
>10	1.2224263217335944	15.064218370724964
>50	0.09864651989762695	4.389812569097901
>100	0.041991424010476334	4.545386986884794
>500	6.665305398488307E-4	0.2411614281140991
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	572	0.2406354121091778	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	428	0.1800558678019722	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	367	0.15439369972739203	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	336	0.14135227005014642	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	308	0.1295729142126342	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	284	0.11947632349476661	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	275	0.11569010197556626	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	264	0.1110624978965436	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	239	0.10054521589876486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.2069127991115E-4	0.0	0.0	0.0	0.0
7	4.2069127991115E-4	0.0	0.0	0.0	0.0
8	4.2069127991115E-4	0.0	0.0	0.0	0.0
9	4.2069127991115E-4	0.0	0.0	0.0	0.0
10	4.2069127991115E-4	0.0	0.0	0.0	0.0
11	4.2069127991115E-4	0.0	0.0	0.0	0.0
12	4.2069127991115E-4	0.0	0.0	0.0	0.0
13	4.2069127991115E-4	0.0	0.0	0.0	0.0
14	4.2069127991115E-4	0.0	0.0	0.0	0.0
15	4.2069127991115E-4	0.0	0.0	0.0	0.0
16	4.2069127991115E-4	0.0	0.0	4.2069127991115E-4	0.0
17	4.2069127991115E-4	0.0	0.0	4.2069127991115E-4	0.0
18	4.2069127991115E-4	0.0	0.0	4.2069127991115E-4	0.0
19	4.2069127991115E-4	0.0	0.0	4.2069127991115E-4	0.0
20	4.2069127991115E-4	0.0	0.0	0.00126207383973345	0.0
21	4.2069127991115E-4	0.0	0.0	0.0016827651196446	0.0
22	4.2069127991115E-4	0.0	0.0	0.00378622151920035	0.0
23	4.2069127991115E-4	0.0	0.0	0.0050482953589338	0.0
24	4.2069127991115E-4	0.0	0.0	0.0075724430384007	0.0
25	4.2069127991115E-4	0.0	0.0	0.00883451687813415	0.0
26	4.2069127991115E-4	0.0	0.0	0.012200047117423351	0.0
27	4.2069127991115E-4	0.0	0.0	0.0403863628714704	0.0
28	4.2069127991115E-4	0.0	0.0	0.15944199508632584	0.0
29	4.2069127991115E-4	0.0	0.0	0.36642210480261167	0.0
30	4.2069127991115E-4	0.0	0.0	0.6184161814693905	0.0
31	4.2069127991115E-4	0.0	0.0	1.2002322215865109	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAATG	30	3.5909703E-4	30.833332	2
CATGCGG	25	0.005487686	29.599998	10
TCTTAGA	25	0.005487686	29.599998	2
AGGTGAC	25	0.005487686	29.599998	30
GGTATCA	310	0.0	27.451612	1
TTGTAAT	35	8.848629E-4	26.42857	1
GTAATGC	35	8.848629E-4	26.42857	3
ATACGGC	50	9.0515405E-6	25.899998	29
ATACACA	60	1.329965E-6	24.666666	37
GTCTTGG	55	1.8944405E-5	23.545454	1
GGCATAT	40	0.0019267085	23.125	8
TTAGCCC	120	0.0	23.124998	29
AGTGGTT	50	2.693375E-4	22.199999	27
CGCGGTA	240	0.0	21.583332	22
GCGGTAA	235	0.0	21.255318	23
TAATGCT	45	0.0038170204	20.555555	4
GTATCAA	415	0.0	20.506023	2
ACGACCA	55	5.126618E-4	20.181818	5
TAATACG	215	0.0	19.790697	27
CGTGCCA	255	0.0	19.588236	10
>>END_MODULE