FastQCFastQC Report
Fri 10 Feb 2017
ERR1632513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31354
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1900.6059832876188046No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1290.4114307584359252No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1240.39548382981437774No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1120.3572112011226638TruSeq Adapter, Index 11 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1040.33169611532818777No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1000.3189385724309498No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC950.3029916438094023No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA930.2966128723607833No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA890.28385532946354536No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC840.26790840084199785No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC790.25196147222045034No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT770.24558270077183134No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA710.22644638642597437No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT650.20731007208011737No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA650.20731007208011737No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG630.20093130063149836No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA620.19774191490718887No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC610.19455252918287938No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT600.1913631434585699No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC590.18817375773426037No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA590.18817375773426037No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC580.18498437200995088No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC580.18498437200995088No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC550.17541621483702238No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA550.17541621483702238No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA540.1722268291127129No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT540.1722268291127129No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC530.16903744338840337No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT530.16903744338840337No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC530.16903744338840337No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA530.16903744338840337No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG520.16584805766409388No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC510.1626586719397844No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC510.1626586719397844No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC490.1562799004911654No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA490.1562799004911654No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA480.1530905147668559No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC470.1499011290425464No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT460.14671174331823691No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG440.14033297186961793No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG440.14033297186961793No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA430.13714358614530842No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA420.13395420042099893No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA410.1307648146966894No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA410.1307648146966894No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA400.12757542897237992No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC400.12757542897237992No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT400.12757542897237992No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG400.12757542897237992No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG390.12438604324807043No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC390.12438604324807043No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC390.12438604324807043No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC380.12119665752376091No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC370.11800727179945142RNA PCR Primer, Index 11 (95% over 22bp)
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC370.11800727179945142No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG360.11481788607514193No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA360.11481788607514193No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC350.11162850035083244No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA350.11162850035083244No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC350.11162850035083244No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG340.10843911462652293No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA340.10843911462652293No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC330.10524972890221343No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG330.10524972890221343No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG320.10206034317790393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA800.032.3751
AGCCTGC250.005416183729.66
CCAGTAC250.005416183729.631
CAGTACC250.005416183729.632
GCGGTAA652.759407E-928.46153823
TAATACG652.759407E-928.46153827
GTAATAC652.759407E-928.46153826
CGGTAAT652.759407E-928.46153824
GGTAATA652.759407E-928.46153825
CAGCAGC706.0845196E-926.42857215
AGGGTGC358.677636E-426.42857235
GCAGCCG706.0845196E-926.42857217
CGCGGTA706.0845196E-926.42857222
GGAGGGT358.677636E-426.42857233
GAGGGTG358.677636E-426.42857234
CGGAGGG508.728595E-625.90000232
CTTATAC508.728595E-625.90000237
ACGGAGG508.728595E-625.90000231
TAACTCC751.2685632E-824.6666664
CTAACTC751.2685632E-824.6666663
Produced by FastQC (version 0.11.5)