##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632513.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31354
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87940932576386	34.0	31.0	34.0	31.0	34.0
2	33.01001467117433	34.0	33.0	34.0	31.0	34.0
3	33.07660904509791	34.0	33.0	34.0	31.0	34.0
4	36.429131849205845	37.0	37.0	37.0	35.0	37.0
5	36.376411303183005	37.0	37.0	37.0	35.0	37.0
6	36.416342412451364	37.0	37.0	37.0	35.0	37.0
7	36.43704152580213	37.0	37.0	37.0	35.0	37.0
8	36.41736301588314	37.0	37.0	37.0	35.0	37.0
9	38.171110544109204	39.0	39.0	39.0	37.0	39.0
10	38.170982968680235	39.0	38.0	39.0	37.0	39.0
11	38.26947119984691	39.0	39.0	39.0	37.0	39.0
12	38.20399311092684	39.0	39.0	39.0	37.0	39.0
13	38.26079607067679	39.0	39.0	39.0	37.0	39.0
14	39.70332333992473	41.0	40.0	41.0	38.0	41.0
15	39.721279581552594	41.0	40.0	41.0	38.0	41.0
16	39.66186132550871	41.0	40.0	41.0	38.0	41.0
17	39.68090195828283	41.0	40.0	41.0	38.0	41.0
18	39.69098041717165	41.0	40.0	41.0	38.0	41.0
19	39.678573706704086	41.0	40.0	41.0	38.0	41.0
20	39.67407667283281	41.0	40.0	41.0	37.0	41.0
21	39.62591694839574	41.0	40.0	41.0	37.0	41.0
22	39.576800408241375	41.0	39.0	41.0	37.0	41.0
23	39.55479364674364	41.0	40.0	41.0	37.0	41.0
24	39.528704471518786	41.0	39.0	41.0	37.0	41.0
25	39.492855775977546	41.0	39.0	41.0	37.0	41.0
26	39.381067806340496	41.0	39.0	41.0	37.0	41.0
27	39.20731007208012	40.0	39.0	41.0	36.0	41.0
28	39.177106589270906	40.0	39.0	41.0	36.0	41.0
29	39.12537475282261	40.0	39.0	41.0	35.0	41.0
30	39.089781208139314	40.0	39.0	41.0	35.0	41.0
31	39.013172163041396	40.0	39.0	41.0	35.0	41.0
32	38.944217643681824	40.0	39.0	41.0	35.0	41.0
33	38.86732155386873	40.0	38.0	41.0	35.0	41.0
34	38.82997384703706	40.0	38.0	41.0	35.0	41.0
35	38.71349748038528	40.0	38.0	41.0	35.0	41.0
36	38.65698156535051	40.0	38.0	41.0	35.0	41.0
37	38.565382407348345	40.0	38.0	41.0	35.0	41.0
38	38.517956241627864	40.0	38.0	41.0	35.0	41.0
39	38.36665178286662	40.0	38.0	41.0	34.0	41.0
40	38.28969190533903	40.0	37.0	41.0	34.0	41.0
41	38.188109970019774	40.0	37.0	41.0	34.0	41.0
42	38.10582381833259	40.0	37.0	41.0	34.0	41.0
43	37.341902149645975	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	1.0
21	2.0
22	6.0
23	16.0
24	18.0
25	35.0
26	44.0
27	59.0
28	89.0
29	145.0
30	178.0
31	223.0
32	284.0
33	435.0
34	633.0
35	975.0
36	1529.0
37	3104.0
38	8155.0
39	15420.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.92275307775723	15.11449894750271	12.814951840275562	29.147796134464503
2	23.225106844421763	19.76143394782165	29.951521336990496	27.06193787076609
3	22.019519040632773	18.584550615551446	28.940486062384384	30.455444281431397
4	15.988390635963516	16.020284493206606	35.111947438923266	32.87937743190661
5	17.7297952414365	31.84282707150603	32.340371244498314	18.087006442559165
6	31.574918670664033	34.80895579511386	16.342412451361866	17.27371308286024
7	28.6438731900236	27.329846271608087	18.683421573005038	25.34285896536327
8	26.542067997703644	28.742744147477197	21.432672067359825	23.282515787459335
9	27.84014798749761	11.743318236907571	16.90055495311603	43.51597882247879
10	20.223894877846526	21.35293742425209	26.924794284620784	31.4983734132806
11	41.350385915672646	18.49524781527078	17.53843209797793	22.61593417107865
12	21.73247432544492	23.301652101805193	25.99987242457103	28.96600114817886
13	38.993429865407926	16.087261593417107	18.68661095872935	26.232697582445617
14	24.45620973400523	19.410601518147605	22.584040313835555	33.54914843401161
15	31.750334885501054	22.217260955539963	18.447407029406136	27.584997129552846
16	24.97926899279199	23.65248453147924	22.49154812783058	28.876698347898195
17	28.14632901703132	23.148561587038337	20.1473496204631	28.557759775467247
18	27.25011162850035	18.830133316323273	22.22363972698858	31.69611532818779
19	29.964278879887736	21.435861453084136	21.78988326848249	26.809976398545636
20	32.37864387319002	18.118900299802256	21.190278752312306	28.312177074695416
21	28.627926261402052	20.051668048733813	20.931938508643235	30.388467181220896
22	29.530522421381644	22.23320788416151	19.31173056069401	28.92453913376284
23	30.305543152388847	20.220705492122217	20.797984308222237	28.6757670472667
24	28.64706257574791	19.49352554697965	22.03546596925432	29.823945908018118
25	30.79670855393251	20.880908337054283	21.049945780442687	27.272437328570515
26	30.729731453722014	21.611277667921158	20.625757479109524	27.0332333992473
27	27.805064744530206	20.884097722778595	24.593353320150538	26.717484212540665
28	27.18632391401416	22.915736429163744	20.520507750207308	29.377431906614788
29	28.028321745231864	24.044778975569304	20.310008292402884	27.616890986795944
30	28.187791031447347	20.258978120813932	23.856605217835046	27.696625629903682
31	29.90686993685016	21.41353575301397	21.60170951074823	27.077884799387636
32	26.36665178286662	20.82668877974102	22.54257829941953	30.264081137972827
33	26.70153728391912	20.874529565605666	23.677999617273713	28.745933533201505
34	27.368118900299805	20.852203865535497	22.963577215028387	28.816100019136314
35	25.983925495949478	23.12942527269248	24.185111947438923	26.70153728391912
36	27.594565286725775	20.46309880716974	24.574217005804684	27.368118900299805
37	28.018753588058942	20.683166422147096	23.26019008738917	28.037889902404796
38	26.191235568029597	19.416980289596225	26.156152325062195	28.235631817311983
39	25.770236652420742	18.447407029406136	26.430439497352808	29.35191682082031
40	25.66498692351853	19.471199846909485	28.51310837532691	26.350704854245073
41	23.524909102506857	19.11079926006251	28.956432991005933	28.4078586464247
42	20.59067423614212	19.780570262167508	31.491994641831983	28.13676085985839
43	19.89219876251834	20.836256936913948	29.556037507176118	29.715506793391594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.5
7	1.0
8	1.5
9	2.0
10	1.5
11	1.0
12	1.0
13	8.5
14	16.0
15	24.5
16	33.0
17	29.0
18	25.0
19	25.0
20	32.0
21	39.0
22	30.0
23	21.0
24	21.0
25	21.0
26	21.0
27	18.5
28	16.0
29	21.5
30	27.0
31	31.5
32	36.0
33	36.0
34	64.5
35	93.0
36	91.5
37	90.0
38	166.0
39	242.0
40	242.0
41	390.5
42	539.0
43	698.5
44	858.0
45	1502.0
46	2146.0
47	2146.0
48	2574.0
49	3002.0
50	3389.5
51	3777.0
52	3889.5
53	4002.0
54	4002.0
55	3742.0
56	3482.0
57	3296.5
58	3111.0
59	2989.0
60	2867.0
61	2867.0
62	2669.0
63	2471.0
64	1961.5
65	1452.0
66	1274.0
67	1096.0
68	1096.0
69	922.0
70	748.0
71	620.0
72	492.0
73	397.0
74	302.0
75	302.0
76	246.0
77	190.0
78	136.5
79	83.0
80	63.0
81	43.0
82	43.0
83	29.5
84	16.0
85	13.5
86	11.0
87	5.5
88	0.0
89	0.0
90	1.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	31354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38049371691013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.70767104353835	46.35772150283856
2	6.97419949320433	7.724692224277605
3	2.7988942639944714	4.650124386043248
4	1.4627965906473164	3.2404158958984497
5	1.0251094217922136	2.838553294635453
6	0.73715733701912	2.4494482362696943
7	0.506795669200645	1.9646616061746507
8	0.3916148352914075	1.735025834024367
9	0.2706749596867081	1.3491101613829177
>10	1.9292789679797282	19.586017732984626
>50	0.16701220916839438	6.002423933150475
>100	0.02879520847730938	2.101805192319959
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	190	0.6059832876188046	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	129	0.4114307584359252	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	124	0.39548382981437774	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	112	0.3572112011226638	TruSeq Adapter, Index 11 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	104	0.33169611532818777	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	100	0.3189385724309498	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	95	0.3029916438094023	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	93	0.2966128723607833	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	89	0.28385532946354536	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	84	0.26790840084199785	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	79	0.25196147222045034	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	77	0.24558270077183134	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	71	0.22644638642597437	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	65	0.20731007208011737	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	65	0.20731007208011737	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	63	0.20093130063149836	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	62	0.19774191490718887	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	61	0.19455252918287938	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	60	0.1913631434585699	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	59	0.18817375773426037	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	59	0.18817375773426037	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	58	0.18498437200995088	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	58	0.18498437200995088	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	55	0.17541621483702238	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	55	0.17541621483702238	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	54	0.1722268291127129	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	54	0.1722268291127129	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	53	0.16903744338840337	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	53	0.16903744338840337	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	53	0.16903744338840337	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	53	0.16903744338840337	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	52	0.16584805766409388	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	51	0.1626586719397844	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	51	0.1626586719397844	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	49	0.1562799004911654	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	49	0.1562799004911654	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	48	0.1530905147668559	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	47	0.1499011290425464	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	46	0.14671174331823691	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	44	0.14033297186961793	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	44	0.14033297186961793	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	43	0.13714358614530842	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	42	0.13395420042099893	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	41	0.1307648146966894	No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA	41	0.1307648146966894	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	40	0.12757542897237992	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	40	0.12757542897237992	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	40	0.12757542897237992	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	40	0.12757542897237992	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	39	0.12438604324807043	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	39	0.12438604324807043	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	39	0.12438604324807043	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	38	0.12119665752376091	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	37	0.11800727179945142	RNA PCR Primer, Index 11 (95% over 22bp)
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	37	0.11800727179945142	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	36	0.11481788607514193	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	36	0.11481788607514193	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	35	0.11162850035083244	No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA	35	0.11162850035083244	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	35	0.11162850035083244	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	34	0.10843911462652293	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	34	0.10843911462652293	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	33	0.10524972890221343	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	33	0.10524972890221343	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	32	0.10206034317790393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.006378771448618996	0.0
25	0.0	0.0	0.0	0.012757542897237992	0.0
26	0.0	0.0	0.0	0.01594692862154749	0.0
27	0.0	0.0	0.0	0.044651400140332975	0.0
28	0.0	0.0	0.0	0.22644638642597437	0.0
29	0.0	0.0	0.0	0.4560821585762582	0.0
30	0.0	0.0	0.0	0.7526950309370415	0.0
31	0.0	0.0	0.0	1.4384129616635837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	80	0.0	32.375	1
AGCCTGC	25	0.0054161837	29.6	6
CCAGTAC	25	0.0054161837	29.6	31
CAGTACC	25	0.0054161837	29.6	32
GCGGTAA	65	2.759407E-9	28.461538	23
TAATACG	65	2.759407E-9	28.461538	27
GTAATAC	65	2.759407E-9	28.461538	26
CGGTAAT	65	2.759407E-9	28.461538	24
GGTAATA	65	2.759407E-9	28.461538	25
CAGCAGC	70	6.0845196E-9	26.428572	15
AGGGTGC	35	8.677636E-4	26.428572	35
GCAGCCG	70	6.0845196E-9	26.428572	17
CGCGGTA	70	6.0845196E-9	26.428572	22
GGAGGGT	35	8.677636E-4	26.428572	33
GAGGGTG	35	8.677636E-4	26.428572	34
CGGAGGG	50	8.728595E-6	25.900002	32
CTTATAC	50	8.728595E-6	25.900002	37
ACGGAGG	50	8.728595E-6	25.900002	31
TAACTCC	75	1.2685632E-8	24.666666	4
CTAACTC	75	1.2685632E-8	24.666666	3
>>END_MODULE