FastQCFastQC Report
Fri 10 Feb 2017
ERR1632512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962246
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA52110.17591381674580706No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA44040.14867097465909312No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA42940.1449575761094791No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA42370.14303336049740636No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA34500.11646568178334951No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA34010.11481153152033963No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG33790.11406885181041683No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA33280.11234718521014123No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC33230.11217839436697694No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT31430.10610192401306305No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA30310.1023210091261833No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT29900.10093692421223625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35650.027.4516141
TTATAAG1153.0559022E-919.3043483
ATACGGC4600.019.30434829
GCGGTAA23100.018.7402623
GTATCAA52600.018.6055132
CTTATAC41300.018.49999837
ATACACA8700.017.64942437
CGGTAAT24800.017.45564724
GCTTATA852.7254633E-517.4117661
TAATACG24450.017.17586927
TCGCTAA650.001580729617.07692314
GGTAATA26000.016.93461625
CGTGCCA26150.016.62523810
AGCCGCG26550.016.30508619
AACTCCG26800.016.2910445
CGCGGTA27050.016.27726422
CGAACTA3550.016.1549324
GTTCTAT1059.352974E-615.8571421
GTATTAT1059.352974E-615.8571421
CTCTATG9000.015.8277781
Produced by FastQC (version 0.11.5)