##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632512.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2962246
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.060438937211835	34.0	33.0	34.0	31.0	34.0
2	33.20073315990637	34.0	33.0	34.0	31.0	34.0
3	33.278652751999665	34.0	34.0	34.0	31.0	34.0
4	36.55514430604345	37.0	37.0	37.0	35.0	37.0
5	36.52502931896946	37.0	37.0	37.0	35.0	37.0
6	36.567288469627435	37.0	37.0	37.0	35.0	37.0
7	36.564249221705424	37.0	37.0	37.0	35.0	37.0
8	36.53931746384331	37.0	37.0	37.0	35.0	37.0
9	38.32533996163722	39.0	39.0	39.0	37.0	39.0
10	38.36602193065667	39.0	39.0	39.0	37.0	39.0
11	38.42825916551158	39.0	39.0	39.0	37.0	39.0
12	38.39670506770876	39.0	39.0	39.0	37.0	39.0
13	38.42903020208315	39.0	39.0	39.0	37.0	39.0
14	39.99499535150018	41.0	40.0	41.0	38.0	41.0
15	39.984224807797865	41.0	40.0	41.0	38.0	41.0
16	39.95405614523575	41.0	40.0	41.0	38.0	41.0
17	39.95284794038037	41.0	40.0	41.0	38.0	41.0
18	39.95285367926904	41.0	40.0	41.0	38.0	41.0
19	39.9580770807016	41.0	40.0	41.0	38.0	41.0
20	39.931963111773975	41.0	40.0	41.0	38.0	41.0
21	39.90076482506855	41.0	40.0	41.0	38.0	41.0
22	39.87086487752874	41.0	40.0	41.0	38.0	41.0
23	39.84070532967215	41.0	40.0	41.0	38.0	41.0
24	39.82426678945638	41.0	40.0	41.0	38.0	41.0
25	39.783338385805905	41.0	40.0	41.0	38.0	41.0
26	39.71031946705304	41.0	40.0	41.0	38.0	41.0
27	39.64048428118394	41.0	40.0	41.0	37.0	41.0
28	39.59886619814829	41.0	40.0	41.0	37.0	41.0
29	39.56633851476211	41.0	40.0	41.0	37.0	41.0
30	39.512826078590365	41.0	40.0	41.0	37.0	41.0
31	39.4600006211503	41.0	39.0	41.0	37.0	41.0
32	39.4150084091598	41.0	39.0	41.0	36.0	41.0
33	39.35962509528243	41.0	39.0	41.0	36.0	41.0
34	39.31964900956909	41.0	39.0	41.0	36.0	41.0
35	39.247930455471966	41.0	39.0	41.0	35.0	41.0
36	39.20058901252631	41.0	39.0	41.0	35.0	41.0
37	39.15001421218899	41.0	39.0	41.0	35.0	41.0
38	39.07775282674025	41.0	39.0	41.0	35.0	41.0
39	38.99401535186477	41.0	39.0	41.0	35.0	41.0
40	38.92015011582428	40.0	39.0	41.0	35.0	41.0
41	38.865647552566536	40.0	39.0	41.0	35.0	41.0
42	38.79519965593675	40.0	38.0	41.0	35.0	41.0
43	38.07835844828552	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	1.0
13	0.0
14	1.0
15	4.0
16	5.0
17	5.0
18	30.0
19	59.0
20	117.0
21	254.0
22	506.0
23	901.0
24	1529.0
25	2260.0
26	3236.0
27	4555.0
28	6412.0
29	8985.0
30	12375.0
31	16049.0
32	21471.0
33	28801.0
34	42905.0
35	68925.0
36	107534.0
37	209055.0
38	552269.0
39	1873996.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.10047173664848	16.575632138586734	12.461659159975234	27.862236964789556
2	20.96540935492866	18.826086692327376	32.86985618344999	27.33864776929397
3	21.332158098955993	19.694144240552607	28.660718927462476	30.312978733028924
4	15.678880147023577	15.680770604467014	33.63545768987451	35.004891558634895
5	17.47494975096599	32.45041093818677	32.93649480833125	17.138144502515996
6	34.70545660286148	33.39756387551878	15.208190001775678	16.688789519844065
7	30.492572190155716	27.518646324444358	19.84126233945459	22.147519145945342
8	27.195243068941604	31.036450044999636	19.812736686959827	21.95557019909893
9	27.808493960326047	13.301191055705704	18.019874109037534	40.870440874930715
10	19.631185256052333	23.182342047216874	29.911053977286155	27.275418719444637
11	38.966041307845465	19.69184868508557	19.141151680177813	22.200958326891147
12	23.04687051649323	23.42489448884394	26.83200517445209	26.696229820210743
13	34.27237305747058	17.824718136171	21.004737621385935	26.898171184972487
14	24.092799855244973	19.68752763950057	23.15185842094141	33.06781408431305
15	29.217559919061415	24.050703418959802	20.396381664453255	26.335354997525528
16	25.896397530792512	23.75329395330435	22.718572326538713	27.63173618936442
17	26.511707670463558	23.550103536303197	22.42055521384787	27.517633579385368
18	26.452056986489307	22.028825425032224	23.585819678716756	27.933297909761716
19	27.909768466224616	22.587860697592298	23.35538641962889	26.146984416554197
20	28.858541795651004	21.354877346445907	23.142439891892842	26.644140966010248
21	27.309244404414756	22.524766680417493	22.40067165252312	27.76531726264463
22	28.07271914621541	23.13146848708716	21.799101087485646	26.996711279211787
23	27.874052323811053	22.434024723132378	22.75601013555255	26.935912817504015
24	27.779326902627265	22.162946629010555	22.47716766264517	27.580558805717015
25	27.830301737262875	22.363706457870144	23.081371364836006	26.72462044003098
26	27.597100308347112	22.85414513176826	22.755672553866223	26.793082006018405
27	27.118848333325456	22.25277711574258	23.83333457113285	26.795039979799114
28	26.426805876351928	23.13649845421346	22.661791086898255	27.774904582536358
29	27.370515480483387	23.42702125346781	22.637080107459003	26.5653831585898
30	26.876093342686598	22.34000822348988	23.879110647799003	26.904787786024524
31	27.368051134173193	22.415221423203878	23.035730320844387	27.180997121778542
32	25.509393885585464	22.426226586178192	23.328008544867647	28.736370983368705
33	25.96823491364323	21.922926050030956	24.612540619516405	27.496298416809406
34	26.116298241266932	21.685707399047885	24.622195455745405	27.57579890393978
35	25.906930079405967	22.77596121321457	24.73744584345797	26.5796628639215
36	26.0030395855037	21.557055018388073	25.320854513770968	27.119050882337252
37	25.957331025174817	20.996095530215925	25.387931994844454	27.658641449764808
38	25.22828961537968	20.401647938759982	26.618923614041506	27.751138831818828
39	24.60237941075792	19.73782731076352	27.855957945423842	27.803835333054717
40	24.276680599788133	20.727211717055237	28.51950850807124	26.47659917508539
41	22.87885611120751	20.013023901458553	29.37213857323126	27.735981414102678
42	21.424621722841383	20.932731447692056	30.12629606048924	27.516350768977322
43	20.988297393261735	21.23871548818025	29.617796766372546	28.15519035218547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	24.5
2	26.0
3	82.0
4	138.0
5	138.0
6	178.0
7	218.0
8	250.0
9	282.0
10	404.0
11	526.0
12	526.0
13	1070.5
14	1615.0
15	2843.0
16	4071.0
17	3958.5
18	3846.0
19	3846.0
20	4674.5
21	5503.0
22	4823.5
23	4144.0
24	4815.0
25	5486.0
26	5486.0
27	6803.5
28	8121.0
29	10856.0
30	13591.0
31	17905.0
32	22219.0
33	22219.0
34	30283.5
35	38348.0
36	44871.5
37	51395.0
38	65837.5
39	80280.0
40	80280.0
41	93940.0
42	107600.0
43	127175.5
44	146751.0
45	182107.5
46	217464.0
47	217464.0
48	244184.5
49	270905.0
50	296059.0
51	321213.0
52	324188.0
53	327163.0
54	327163.0
55	309028.5
56	290894.0
57	275112.5
58	259331.0
59	239958.0
60	220585.0
61	220585.0
62	199575.5
63	178566.0
64	150308.0
65	122050.0
66	106262.5
67	90475.0
68	90475.0
69	77017.5
70	63560.0
71	53423.0
72	43286.0
73	34473.5
74	25661.0
75	25661.0
76	20859.0
77	16057.0
78	12671.5
79	9286.0
80	7445.0
81	5604.0
82	5604.0
83	4344.0
84	3084.0
85	2366.0
86	1648.0
87	1190.0
88	732.0
89	732.0
90	547.5
91	363.0
92	230.0
93	97.0
94	63.5
95	30.0
96	30.0
97	19.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2962246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.00242163425011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.20084456116054	36.61038642705891
2	9.317335276189114	8.199706306610912
3	2.5862128857133184	3.4139888949926442
4	1.202121396198543	2.115850101243269
5	0.7231635646030987	1.591047404009641
6	0.4621102322646395	1.2200381568965948
7	0.3409321204387053	1.0501287238541994
8	0.24617961750772852	0.8665999461866793
9	0.20673388073130616	0.8187112247421339
>10	1.3541283429357427	11.757024575900038
>50	0.1770697996728472	5.42351721626063
>100	0.15423679980506017	13.680036834707801
>500	0.01915943928748888	5.731262272037125
>1k	0.009695137952703695	7.34526839529209
>5k	7.694553930717218E-5	0.17643352020736724
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	5211	0.17591381674580706	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	4404	0.14867097465909312	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	4294	0.1449575761094791	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	4237	0.14303336049740636	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3450	0.11646568178334951	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	3401	0.11481153152033963	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	3379	0.11406885181041683	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	3328	0.11234718521014123	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	3323	0.11217839436697694	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	3143	0.10610192401306305	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	3031	0.1023210091261833	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	2990	0.10093692421223625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	6.751633726570988E-5	0.0	0.0	0.0	0.0
5	1.012745058985648E-4	0.0	0.0	0.0	0.0
6	1.012745058985648E-4	0.0	0.0	0.0	0.0
7	1.012745058985648E-4	0.0	0.0	0.0	0.0
8	1.3503267453141975E-4	0.0	0.0	0.0	0.0
9	1.3503267453141975E-4	0.0	0.0	0.0	0.0
10	1.3503267453141975E-4	0.0	3.375816863285494E-5	0.0	0.0
11	1.6879084316427466E-4	3.375816863285494E-5	3.375816863285494E-5	0.0	0.0
12	2.025490117971296E-4	3.375816863285494E-5	3.375816863285494E-5	3.375816863285494E-5	3.375816863285494E-5
13	2.3630718042998454E-4	3.375816863285494E-5	3.375816863285494E-5	6.751633726570988E-5	3.375816863285494E-5
14	2.3630718042998454E-4	3.375816863285494E-5	3.375816863285494E-5	6.751633726570988E-5	3.375816863285494E-5
15	2.700653490628395E-4	3.375816863285494E-5	3.375816863285494E-5	1.3503267453141975E-4	3.375816863285494E-5
16	2.700653490628395E-4	3.375816863285494E-5	3.375816863285494E-5	3.713398549614043E-4	3.375816863285494E-5
17	2.700653490628395E-4	3.375816863285494E-5	3.375816863285494E-5	9.452287217199381E-4	3.375816863285494E-5
18	2.700653490628395E-4	3.375816863285494E-5	3.375816863285494E-5	0.0013503267453141973	1.012745058985648E-4
19	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.0016879084316427468	1.012745058985648E-4
20	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.0023293136356669904	1.012745058985648E-4
21	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.003207026020121219	1.012745058985648E-4
22	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.0048274181144982555	1.012745058985648E-4
23	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.0061439866911795985	1.012745058985648E-4
24	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.008101960471885184	1.012745058985648E-4
25	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.009891143409426496	1.3503267453141975E-4
26	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.014380979837596203	1.3503267453141975E-4
27	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.046079900183846986	1.3503267453141975E-4
28	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.17115391496857452	1.3503267453141975E-4
29	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.35594613006482245	1.3503267453141975E-4
30	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	0.6059253687911133	1.3503267453141975E-4
31	3.038235176956944E-4	3.375816863285494E-5	3.375816863285494E-5	1.3563019411622128	1.3503267453141975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3565	0.0	27.451614	1
TTATAAG	115	3.0559022E-9	19.304348	3
ATACGGC	460	0.0	19.304348	29
GCGGTAA	2310	0.0	18.74026	23
GTATCAA	5260	0.0	18.605513	2
CTTATAC	4130	0.0	18.499998	37
ATACACA	870	0.0	17.649424	37
CGGTAAT	2480	0.0	17.455647	24
GCTTATA	85	2.7254633E-5	17.411766	1
TAATACG	2445	0.0	17.175869	27
TCGCTAA	65	0.0015807296	17.076923	14
GGTAATA	2600	0.0	16.934616	25
CGTGCCA	2615	0.0	16.625238	10
AGCCGCG	2655	0.0	16.305086	19
AACTCCG	2680	0.0	16.291044	5
CGCGGTA	2705	0.0	16.277264	22
CGAACTA	355	0.0	16.15493	24
GTTCTAT	105	9.352974E-6	15.857142	1
GTATTAT	105	9.352974E-6	15.857142	1
CTCTATG	900	0.0	15.827778	1
>>END_MODULE