FastQCFastQC Report
Fri 10 Feb 2017
ERR1632511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888283
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA40770.14115652794411074No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA35070.12142161969585391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.027.5090181
CTTATAC40000.018.91625237
ATACACA8350.018.61077937
GTATCAA36850.018.5251032
GCGGTAA18050.017.62880923
TAATACG17250.017.26666627
TATACAC12400.017.00806437
ATACGGC3500.016.91428629
GTAACGT11650.016.35622226
CGGTAAT19450.016.26478224
TCTTATA70050.016.2155637
GCTATAC803.3847828E-416.18753
GGTAATA20250.016.07901225
AACGTCA11250.015.95111228
ACTTATA1201.9378713E-615.41666731
AATACGG19650.015.34605528
GTAATAC20600.015.26699126
GTATTAG7950.015.1257861
TATTAGA5750.015.1217392
CGTGCCA22250.015.04943810
Produced by FastQC (version 0.11.5)