FastQCFastQC Report
Fri 10 Feb 2017
ERR1632510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850357
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA43790.23665703429122056No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA34260.18515345957563864No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA26960.14570161325625272No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23870.12900213310188252No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20950.11322139457412812No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19810.1070604213132925No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18820.10171010242888265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.029.567331
ATACGGC2700.021.92592629
GTATCAA30300.021.8580862
GGACGTA2050.021.65853527
GCGGTAA18350.018.95367823
AAACACG2250.018.91111215
GTAGATC500.007036464318.53
TATACAT609.2397205E-418.55
TAGTACT609.2397205E-418.54
CTTATAC24650.018.0121737
TATACAC7900.017.56329237
CGCGGTA20450.017.18826522
TAATACG18950.017.18205827
TATTAGA2600.017.0769232
ACGTATA2500.017.0229
GGTAATA21100.016.921825
CGGTAAT20650.016.84261524
CGAAAGT2700.016.44444520
GACGTAT2600.016.36538528
TAATCAG803.3839606E-416.18755
Produced by FastQC (version 0.11.5)