FastQCFastQC Report
Fri 10 Feb 2017
ERR1632509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664562
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA41150.24721217954032354No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA32270.19386481248520632No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA25380.15247254232644983No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA25150.15109079745903126No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC22840.13721327292104468No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22170.13318819004639057No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA21360.12832204507852515No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19450.11684755509257089No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19240.11558596195275395No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18400.1105395893934861No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18050.10843693416045784No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17980.10801640311385216No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17850.10723541688444167No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17400.10453200301340532No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT17280.10381109264779563No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17160.10309018228218593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.028.172591
TTTTACG652.6840462E-622.769234
ATCTTAC1500.022.21
ATACGGC2550.021.03921529
TGTTTAC1303.274181E-1119.92307714
AACGACC1600.019.6562537
GTATCAA28150.019.452932
CTTATAC22000.019.25681937
CTAGACA902.153396E-618.54
ATACACA4300.018.537
AGTATTA1351.1532393E-917.81481615
TATACAC6550.017.79389437
TACCTTA1258.58563E-917.76000231
GCGGTAA15950.017.51410523
GGTAATA17100.017.30994225
CTCTATG3850.017.2987021
TAATACG15350.017.23452827
GTTTAGG1201.0420081E-716.9583321
CGTGCCA17350.016.84726110
AACGTCA8800.016.81818228
Produced by FastQC (version 0.11.5)