##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632509.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664562
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06955883890176	34.0	33.0	34.0	31.0	34.0
2	33.20019921156436	34.0	33.0	34.0	31.0	34.0
3	33.27853753720198	34.0	34.0	34.0	31.0	34.0
4	36.549049539758805	37.0	37.0	37.0	35.0	37.0
5	36.519095113309085	37.0	37.0	37.0	35.0	37.0
6	36.564175440746574	37.0	37.0	37.0	35.0	37.0
7	36.564708914417125	37.0	37.0	37.0	35.0	37.0
8	36.54190111272515	37.0	37.0	37.0	35.0	37.0
9	38.3310456444398	39.0	39.0	39.0	37.0	39.0
10	38.3692995514736	39.0	39.0	39.0	37.0	39.0
11	38.434253575415035	39.0	39.0	39.0	37.0	39.0
12	38.39760249242744	39.0	39.0	39.0	37.0	39.0
13	38.43247893439836	39.0	39.0	39.0	37.0	39.0
14	40.0002054594542	41.0	40.0	41.0	38.0	41.0
15	39.99590823291653	41.0	40.0	41.0	38.0	41.0
16	39.9599726534668	41.0	40.0	41.0	38.0	41.0
17	39.96446752959638	41.0	40.0	41.0	38.0	41.0
18	39.96026342064759	41.0	40.0	41.0	38.0	41.0
19	39.957905442993415	41.0	40.0	41.0	38.0	41.0
20	39.934811680189746	41.0	40.0	41.0	38.0	41.0
21	39.9038690057805	41.0	40.0	41.0	38.0	41.0
22	39.87694961197	41.0	40.0	41.0	38.0	41.0
23	39.849594067388296	41.0	40.0	41.0	38.0	41.0
24	39.82880301244411	41.0	40.0	41.0	38.0	41.0
25	39.78323126444074	41.0	40.0	41.0	38.0	41.0
26	39.70477639162735	41.0	40.0	41.0	38.0	41.0
27	39.62489111249686	41.0	40.0	41.0	37.0	41.0
28	39.58585561847501	41.0	40.0	41.0	37.0	41.0
29	39.554392687085254	41.0	40.0	41.0	37.0	41.0
30	39.51343056011131	41.0	40.0	41.0	37.0	41.0
31	39.449372868057786	41.0	39.0	41.0	37.0	41.0
32	39.41626746255171	41.0	39.0	41.0	36.0	41.0
33	39.33907117908495	41.0	39.0	41.0	36.0	41.0
34	39.30785455873677	41.0	39.0	41.0	36.0	41.0
35	39.22721352523967	41.0	39.0	41.0	35.0	41.0
36	39.17623494949422	41.0	39.0	41.0	35.0	41.0
37	39.11855971721089	41.0	39.0	41.0	35.0	41.0
38	39.039121402507085	41.0	39.0	41.0	35.0	41.0
39	38.947454044967984	41.0	39.0	41.0	35.0	41.0
40	38.863589941377974	40.0	39.0	41.0	35.0	41.0
41	38.804430234500124	40.0	38.0	41.0	35.0	41.0
42	38.731263839977125	40.0	38.0	41.0	35.0	41.0
43	37.999272481289374	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	1.0
14	3.0
15	7.0
16	10.0
17	8.0
18	19.0
19	34.0
20	78.0
21	138.0
22	277.0
23	523.0
24	846.0
25	1291.0
26	1878.0
27	2564.0
28	3606.0
29	5219.0
30	6841.0
31	9033.0
32	11968.0
33	16120.0
34	24037.0
35	38374.0
36	60466.0
37	118770.0
38	321378.0
39	1041068.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.08937606409374	15.988830695402154	11.975762993508202	27.946030246995907
2	21.9055823694161	18.724144850116726	30.79903301889626	28.57123976157091
3	22.428242384483124	19.09469277804011	27.748801186137857	30.72826365133891
4	16.341055484866292	14.833091227602216	32.87261153384494	35.953241753686555
5	17.975779814750066	32.16191406508139	32.52284985479664	17.33945626537191
6	36.15906166306812	32.26194037830973	14.464225423865257	17.11477253475689
7	31.11172788997947	26.735141136226826	19.088985570979034	23.064145402814674
8	28.53909917443748	29.147667674739665	19.459533498902417	22.853699651920444
9	28.073931761027826	12.952416311317933	17.28424654653897	41.68940538111527
10	20.299333998973903	22.716846834182206	28.98864686325892	27.99517230358497
11	39.57599656846666	19.43111761532463	18.309861693346356	22.68302412286235
12	24.338955232667814	22.974271910568667	25.460871989147897	27.22590086761563
13	35.67485020083361	17.31770880267602	20.15929716045422	26.848143836036147
14	24.882581724201323	19.22860187845211	22.05294846331948	33.83586793402709
15	29.773417872088874	23.87444865375997	19.395612779818354	26.956520694332802
16	26.526918192293223	23.315743120412456	21.84178180205964	28.31555688523467
17	27.68812456369904	22.682663667679545	21.18347048653039	28.445741282091024
18	26.890677547607115	21.103389360083913	23.056095237065367	28.949837855243604
19	29.356671604902672	21.66113367961061	22.689392164425236	26.292802551061477
20	30.466573188622593	20.443756375551043	21.573242690870032	27.516427744956328
21	28.091894444304266	21.458197411691486	21.86184714056911	28.58806100343514
22	28.764383663690506	22.22482550965359	21.17067432754082	27.84011649911508
23	28.836414624387675	21.43861268009242	21.605383278003462	28.119589417516437
24	28.353464755292983	21.200111500803214	22.26844058677298	28.177983157130825
25	28.842121831448754	21.795943917979624	21.8831139963546	27.478820254217023
26	28.690189971896512	22.09331944379362	21.810962883929825	27.405527700380038
27	27.82744049185311	21.029135592426115	23.149152750092817	27.994271165627953
28	26.95994501856945	22.632260017950667	21.37553302310157	29.032261940378312
29	28.151910232241274	23.04756446440565	21.676813480062624	27.123711823290453
30	28.08919103043323	21.37391097477895	22.573025216243074	27.963872778544747
31	27.713897109269585	22.167813514906623	22.47390004097174	27.64438933485205
32	26.14105091910064	21.427799024608277	22.47744451693599	29.953705539355095
33	26.370961249866333	21.869416699408013	23.480050607907664	28.279571442817993
34	27.487591330331945	20.78690970958126	23.174264461161556	28.551234498925243
35	26.543919661748856	22.04195458024393	23.410002150715925	28.004123607291287
36	26.445395245115535	21.38418394748889	24.65098926924921	27.51943153814637
37	26.982713770949957	20.269055763618297	24.542251955769746	28.205978509662
38	26.456449204054884	20.1203680007113	25.074163653862097	28.349019141371723
39	25.440025664409017	19.263866410503184	26.76602013022044	28.53008779486736
40	25.196898643607145	19.9566011959903	27.272940268971656	27.5735598914309
41	23.33088223809026	19.655861421803454	28.393655508175726	28.619600831930565
42	22.235038406499726	20.352320910846217	29.387190143713482	28.02545053894057
43	22.291810097791494	20.83671260067213	28.36253621072691	28.508941090809476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	27.5
2	21.0
3	50.0
4	79.0
5	79.0
6	113.5
7	148.0
8	137.5
9	127.0
10	211.0
11	295.0
12	295.0
13	550.5
14	806.0
15	1316.5
16	1827.0
17	1763.0
18	1699.0
19	1699.0
20	2140.0
21	2581.0
22	2229.0
23	1877.0
24	2228.0
25	2579.0
26	2579.0
27	3056.0
28	3533.0
29	4496.0
30	5459.0
31	7095.0
32	8731.0
33	8731.0
34	12014.0
35	15297.0
36	17493.5
37	19690.0
38	26006.0
39	32322.0
40	32322.0
41	39002.0
42	45682.0
43	55070.5
44	64459.0
45	84055.0
46	103651.0
47	103651.0
48	121213.5
49	138776.0
50	154147.5
51	169519.0
52	174078.5
53	178638.0
54	178638.0
55	169750.0
56	160862.0
57	155580.0
58	150298.0
59	148842.0
60	147386.0
61	147386.0
62	136095.5
63	124805.0
64	108283.5
65	91762.0
66	79653.0
67	67544.0
68	67544.0
69	59013.5
70	50483.0
71	42036.0
72	33589.0
73	26394.5
74	19200.0
75	19200.0
76	14082.0
77	8964.0
78	7095.5
79	5227.0
80	4147.0
81	3067.0
82	3067.0
83	2428.0
84	1789.0
85	1371.0
86	953.0
87	703.0
88	453.0
89	453.0
90	338.0
91	223.0
92	152.0
93	81.0
94	52.0
95	23.0
96	23.0
97	16.0
98	9.0
99	11.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1664562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.70894919403457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.20152598315758	33.43554117325385
2	8.188016328714985	6.502750487937376
3	2.3124435489576003	2.7547411019889103
4	1.1201398563825589	1.7791830658923282
5	0.7098650975558319	1.409399854673146
6	0.4962220674877922	1.182267412009892
7	0.3444926214420656	0.9575608001793936
8	0.27158977047464494	0.8627635517917749
9	0.21558919100615673	0.7704738209201843
>10	1.6588149818605717	13.28887029728865
>50	0.22999514217151257	6.489081819539509
>100	0.21555677836553122	17.667366659108037
>500	0.02525222545648371	6.74771087082864
>1k	0.010496406966851663	6.1522890845883484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	4115	0.24721217954032354	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3227	0.19386481248520632	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2538	0.15247254232644983	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2515	0.15109079745903126	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2284	0.13721327292104468	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2217	0.13318819004639057	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2136	0.12832204507852515	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1945	0.11684755509257089	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1924	0.11558596195275395	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1840	0.1105395893934861	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1805	0.10843693416045784	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1798	0.10801640311385216	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1785	0.10723541688444167	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1740	0.10453200301340532	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1728	0.10381109264779563	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1716	0.10309018228218593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	6.007586380080766E-5	0.0	0.0	0.0	0.0
9	1.2015172760161532E-4	0.0	0.0	0.0	0.0
10	2.4030345520323064E-4	0.0	0.0	6.007586380080766E-5	0.0
11	2.4030345520323064E-4	0.0	0.0	6.007586380080766E-5	0.0
12	2.4030345520323064E-4	0.0	0.0	6.007586380080766E-5	0.0
13	2.4030345520323064E-4	0.0	0.0	1.2015172760161532E-4	0.0
14	2.4030345520323064E-4	0.0	0.0	1.8022759140242297E-4	0.0
15	2.4030345520323064E-4	0.0	0.0	3.6045518280484594E-4	0.0
16	2.4030345520323064E-4	0.0	0.0	6.007586380080766E-4	6.007586380080766E-5
17	3.003793190040383E-4	0.0	0.0	8.410620932113072E-4	6.007586380080766E-5
18	3.003793190040383E-4	6.007586380080766E-5	0.0	0.0010212896846137302	6.007586380080766E-5
19	3.6045518280484594E-4	6.007586380080766E-5	0.0	0.0012015172760161531	6.007586380080766E-5
20	3.6045518280484594E-4	6.007586380080766E-5	0.0	0.0018022759140242299	6.007586380080766E-5
21	3.6045518280484594E-4	6.007586380080766E-5	0.0	0.003184020781442806	6.007586380080766E-5
22	4.205310466056536E-4	6.007586380080766E-5	0.0	0.004926220831666228	6.007586380080766E-5
23	4.205310466056536E-4	6.007586380080766E-5	0.0	0.005707207061076728	1.2015172760161532E-4
24	4.205310466056536E-4	6.007586380080766E-5	0.0	0.007869938157905803	1.2015172760161532E-4
25	4.205310466056536E-4	6.007586380080766E-5	0.0	0.009732289935730842	1.2015172760161532E-4
26	4.205310466056536E-4	6.007586380080766E-5	0.0	0.01435813144839303	1.8022759140242297E-4
27	4.205310466056536E-4	6.007586380080766E-5	0.0	0.042293408115768594	1.8022759140242297E-4
28	4.205310466056536E-4	1.8022759140242297E-4	0.0	0.1615439977603718	1.8022759140242297E-4
29	4.205310466056536E-4	1.8022759140242297E-4	0.0	0.3403898442953762	1.8022759140242297E-4
30	4.205310466056536E-4	1.8022759140242297E-4	0.0	0.5590659885303161	1.8022759140242297E-4
31	4.205310466056536E-4	1.8022759140242297E-4	0.0	1.1998952276935313	1.8022759140242297E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1970	0.0	28.17259	1
TTTTACG	65	2.6840462E-6	22.76923	4
ATCTTAC	150	0.0	22.2	1
ATACGGC	255	0.0	21.039215	29
TGTTTAC	130	3.274181E-11	19.923077	14
AACGACC	160	0.0	19.65625	37
GTATCAA	2815	0.0	19.45293	2
CTTATAC	2200	0.0	19.256819	37
CTAGACA	90	2.153396E-6	18.5	4
ATACACA	430	0.0	18.5	37
AGTATTA	135	1.1532393E-9	17.814816	15
TATACAC	655	0.0	17.793894	37
TACCTTA	125	8.58563E-9	17.760002	31
GCGGTAA	1595	0.0	17.514105	23
GGTAATA	1710	0.0	17.309942	25
CTCTATG	385	0.0	17.298702	1
TAATACG	1535	0.0	17.234528	27
GTTTAGG	120	1.0420081E-7	16.958332	1
CGTGCCA	1735	0.0	16.847261	10
AACGTCA	880	0.0	16.818182	28
>>END_MODULE