FastQCFastQC Report
Fri 10 Feb 2017
ERR1632507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680414
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA34910.20774642439303648No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA29620.17626608680956002No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA22830.13585937751054206No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18770.11169866473380964No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT17720.10545020453293058No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT17180.10223671071533562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.029.2772561
ATACGGC3750.026.14666629
TAAACCG751.3747922E-824.6666665
GCGGTAA15700.021.91719823
GGTAATA17150.020.38775425
CGGTAAT16850.020.31157124
CGCGGTA16950.020.30088622
CTTATAC28750.020.0765237
GTCTAGT656.904555E-519.9230771
AACTCCG17300.019.8901735
TAATACG15650.019.85942527
TAACTCC17400.019.7758644
TAATAGT759.269948E-619.7333344
CTAACTC17700.019.5451983
GCTAACT17900.019.2234632
CGTGCCA18250.019.05753310
AATACGG16600.018.7228928
GTAATAC18500.018.526
CGTTAGA609.239235E-418.51
TAATTTA801.6173946E-518.515
Produced by FastQC (version 0.11.5)