##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632507.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1680414
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.029717081623936	34.0	33.0	34.0	31.0	34.0
2	33.16447673013912	34.0	33.0	34.0	31.0	34.0
3	33.24862861175877	34.0	34.0	34.0	31.0	34.0
4	36.538848164797486	37.0	37.0	37.0	35.0	37.0
5	36.50627761968182	37.0	37.0	37.0	35.0	37.0
6	36.544152809962306	37.0	37.0	37.0	35.0	37.0
7	36.53841493822355	37.0	37.0	37.0	35.0	37.0
8	36.51288849057435	37.0	37.0	37.0	35.0	37.0
9	38.30820916750277	39.0	39.0	39.0	37.0	39.0
10	38.337656077609445	39.0	39.0	39.0	37.0	39.0
11	38.408007788556866	39.0	39.0	39.0	37.0	39.0
12	38.362765366153816	39.0	39.0	39.0	37.0	39.0
13	38.40180098475733	39.0	39.0	39.0	37.0	39.0
14	39.951485169726034	41.0	40.0	41.0	38.0	41.0
15	39.941047265733324	41.0	40.0	41.0	38.0	41.0
16	39.90826784351951	41.0	40.0	41.0	38.0	41.0
17	39.90253116196366	41.0	40.0	41.0	38.0	41.0
18	39.89885468699975	41.0	40.0	41.0	38.0	41.0
19	39.900522133236215	41.0	40.0	41.0	38.0	41.0
20	39.86484580585498	41.0	40.0	41.0	38.0	41.0
21	39.83460266339128	41.0	40.0	41.0	38.0	41.0
22	39.804028054991214	41.0	40.0	41.0	38.0	41.0
23	39.758497608327474	41.0	40.0	41.0	38.0	41.0
24	39.73693149426272	41.0	40.0	41.0	38.0	41.0
25	39.687512124988245	41.0	40.0	41.0	37.0	41.0
26	39.593970295415296	41.0	40.0	41.0	37.0	41.0
27	39.5013722808784	41.0	40.0	41.0	37.0	41.0
28	39.44855077379741	41.0	39.0	41.0	37.0	41.0
29	39.39083285428472	41.0	39.0	41.0	36.0	41.0
30	39.33829580091572	41.0	39.0	41.0	36.0	41.0
31	39.26488829538435	41.0	39.0	41.0	36.0	41.0
32	39.192115157336225	41.0	39.0	41.0	35.0	41.0
33	39.115124606198236	41.0	39.0	41.0	35.0	41.0
34	39.049663356768036	41.0	39.0	41.0	35.0	41.0
35	38.956835636932325	40.0	39.0	41.0	35.0	41.0
36	38.88768244016058	40.0	39.0	41.0	35.0	41.0
37	38.81448202645301	40.0	38.0	41.0	35.0	41.0
38	38.7237389119586	40.0	38.0	41.0	35.0	41.0
39	38.6201977607899	40.0	38.0	41.0	35.0	41.0
40	38.522938394943154	40.0	38.0	41.0	35.0	41.0
41	38.44369958831574	40.0	37.0	41.0	35.0	41.0
42	38.351723444341694	40.0	37.0	41.0	35.0	41.0
43	37.5764329504515	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	2.0
12	0.0
13	2.0
14	2.0
15	2.0
16	1.0
17	5.0
18	19.0
19	50.0
20	95.0
21	187.0
22	384.0
23	692.0
24	1058.0
25	1591.0
26	2250.0
27	2990.0
28	4349.0
29	5798.0
30	7809.0
31	10335.0
32	13330.0
33	18365.0
34	27275.0
35	43929.0
36	74181.0
37	145594.0
38	346960.0
39	973158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38691893783317	15.439469083214016	11.454974785975361	28.718637192977447
2	21.758269093211556	18.16826091665506	31.733906049342604	28.33956394079078
3	21.715898582135118	18.57667217721347	27.42586053198795	32.281568708663464
4	16.339366370430145	14.516125192958402	32.41623790327859	36.72827053333286
5	18.28567245928682	31.973013793029576	31.850543973092343	17.890769774591263
6	36.43423584902292	32.213073683032874	14.433764536596339	16.918925931347868
7	32.307098131769905	26.429320393664895	18.42593551351036	22.837645961054836
8	28.43186262432948	30.438272949404134	18.791619208123713	22.33824521814267
9	28.37187740640104	12.26751264866872	16.559193151211545	42.80141679371869
10	20.102367630833832	22.220060056628903	28.307607530049143	29.369964782488122
11	41.35712985014407	18.511093099676625	18.13939898144148	21.99237806873782
12	23.884114271840154	21.892105159799907	25.97996684150453	28.243813726855404
13	36.29504395940524	16.354957766359956	19.88349299636875	27.466505277866048
14	24.870954419565656	17.78073736591102	21.957862764771065	35.390445449752264
15	30.420658242552136	22.487613171516067	18.917719085891928	28.17400950003987
16	27.323028729824912	22.45321688583885	21.12324700936793	29.10050737496831
17	28.162167180230586	21.99826947407008	21.132768472531176	28.706794873168164
18	28.17799661273948	20.303568049302136	21.87788247419981	29.640552863758572
19	29.85288149229892	21.078912696514074	21.54641653782937	27.521789273357637
20	30.38060858812174	20.247034361770375	21.186326702824424	28.18603034728347
21	28.88264439596433	20.84153071802544	20.77071483574881	29.505110050261425
22	29.648705616592103	21.383837554316973	20.110520383667357	28.85693644542357
23	29.518856662703357	20.909728197932175	20.93662633136834	28.634788807996124
24	29.09794848174319	20.252211657365386	21.14943103306685	29.50040882782457
25	29.517785498097492	20.600518681705818	21.45923564074091	28.422460179455776
26	29.07021722028024	21.26493828306596	21.49476260016877	28.170081896485033
27	28.441741142361348	20.807193941492987	22.358418818219796	28.392646097925866
28	28.290766442079153	21.76874270269112	20.937637986829436	29.002852868400286
29	28.452452788419997	22.37460530559731	21.182280080980046	27.99066182500265
30	28.23857692211562	21.076770367302345	22.204766206422942	28.479886504159097
31	28.82670579988027	21.115927384561186	21.39222834372958	28.665138471828968
32	26.78530409768069	21.111345180413874	22.417927962990074	29.685422758915365
33	27.22602882384936	20.66948978049457	23.175360357626158	28.929121038029916
34	27.63765357822537	20.41407653114054	23.323895182972766	28.624374707661325
35	26.947049953166303	21.343847409031348	23.75652666545268	27.95257597234967
36	27.037265816638044	20.258281590131954	24.453200223278312	28.25125236995169
37	27.000013092011848	19.638255810770442	24.326921818075782	29.034809279141925
38	25.660283715798606	19.375106372596278	26.02632446528058	28.938285446324535
39	25.224557757790638	18.407428169486806	27.203236821402342	29.164777251320213
40	25.017525443134847	19.019598741738644	28.24696771152823	27.71590810359828
41	23.611205333923664	18.79721306773212	29.060874284551307	28.53070731379291
42	22.247315244933688	19.44276827020008	29.992549455074762	28.31736702979147
43	21.36009340555363	19.49829030227075	29.780815917982117	29.360800374193502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.0
2	6.0
3	31.0
4	56.0
5	56.0
6	78.5
7	101.0
8	102.5
9	104.0
10	141.5
11	179.0
12	179.0
13	391.5
14	604.0
15	1097.0
16	1590.0
17	1515.5
18	1441.0
19	1441.0
20	1687.5
21	1934.0
22	1741.0
23	1548.0
24	1838.5
25	2129.0
26	2129.0
27	2673.5
28	3218.0
29	4257.5
30	5297.0
31	7017.0
32	8737.0
33	8737.0
34	11743.0
35	14749.0
36	17377.0
37	20005.0
38	25606.5
39	31208.0
40	31208.0
41	38565.5
42	45923.0
43	54523.5
44	63124.0
45	80867.5
46	98611.0
47	98611.0
48	112686.5
49	126762.0
50	138933.0
51	151104.0
52	157373.5
53	163643.0
54	163643.0
55	159877.5
56	156112.0
57	153847.0
58	151582.0
59	148880.0
60	146178.0
61	146178.0
62	138147.5
63	130117.0
64	115749.0
65	101381.0
66	91342.5
67	81304.0
68	81304.0
69	71427.0
70	61550.0
71	52408.0
72	43266.0
73	35569.5
74	27873.0
75	27873.0
76	22732.5
77	17592.0
78	13984.0
79	10376.0
80	8023.0
81	5670.0
82	5670.0
83	4313.5
84	2957.0
85	2178.0
86	1399.0
87	1005.0
88	611.0
89	611.0
90	431.5
91	252.0
92	166.0
93	80.0
94	55.0
95	30.0
96	30.0
97	18.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1680414.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.906326618188544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.53869805463255	51.842155107736154
2	7.695351243087778	9.220004500201991
3	2.293037072388633	4.121022834183849
4	0.992670075074918	2.378688709661591
5	0.5667677285351452	1.6976486331137617
6	0.34200336043755997	1.2292899008934317
7	0.2555527126937191	1.071645699235582
8	0.17960964971643825	0.8607803471753109
9	0.14515877581796843	0.7826336132082862
>10	0.8255530489277584	9.485027633138394
>50	0.0912826499837369	3.8285156077567764
>100	0.06435407162596464	7.879784060844765
>500	0.00627578095903833	2.5723352383674047
>1k	0.00368577611880029	3.0304681144826247
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3491	0.20774642439303648	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2962	0.17626608680956002	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2283	0.13585937751054206	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1877	0.11169866473380964	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1772	0.10545020453293058	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1718	0.10223671071533562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.950914477027685E-5	0.0
4	0.0	0.0	0.0	5.950914477027685E-5	0.0
5	0.0	0.0	0.0	5.950914477027685E-5	0.0
6	0.0	0.0	0.0	5.950914477027685E-5	0.0
7	0.0	0.0	0.0	1.190182895405537E-4	0.0
8	1.7852743431083054E-4	0.0	0.0	1.190182895405537E-4	5.950914477027685E-5
9	1.7852743431083054E-4	0.0	0.0	1.190182895405537E-4	5.950914477027685E-5
10	1.7852743431083054E-4	0.0	0.0	1.190182895405537E-4	1.7852743431083054E-4
11	1.7852743431083054E-4	0.0	0.0	1.190182895405537E-4	1.7852743431083054E-4
12	2.9754572385138424E-4	0.0	0.0	1.7852743431083054E-4	1.7852743431083054E-4
13	2.9754572385138424E-4	0.0	0.0	2.380365790811074E-4	1.7852743431083054E-4
14	3.570548686216611E-4	0.0	0.0	4.165640133919379E-4	1.7852743431083054E-4
15	3.570548686216611E-4	0.0	0.0	5.950914477027685E-4	1.7852743431083054E-4
16	4.165640133919379E-4	0.0	0.0	0.001547237764027198	2.9754572385138424E-4
17	4.165640133919379E-4	0.0	0.0	0.0017852743431083055	2.9754572385138424E-4
18	4.760731581622148E-4	0.0	0.0	0.001963801777419136	2.9754572385138424E-4
19	4.760731581622148E-4	0.0	0.0	0.0028564389489732886	2.9754572385138424E-4
20	4.760731581622148E-4	0.0	0.0	0.004284658423459933	2.9754572385138424E-4
21	4.760731581622148E-4	0.0	0.0	0.007081588227662945	2.9754572385138424E-4
22	4.760731581622148E-4	0.0	0.0	0.010830664348190387	2.9754572385138424E-4
23	4.760731581622148E-4	0.0	0.0	0.012734956980839245	2.9754572385138424E-4
24	4.760731581622148E-4	0.0	0.0	0.014996304482109766	2.9754572385138424E-4
25	4.760731581622148E-4	0.0	0.0	0.016305505667055856	2.9754572385138424E-4
26	4.760731581622148E-4	0.0	0.0	0.019280962905569698	2.9754572385138424E-4
27	4.760731581622148E-4	0.0	0.0	0.05028522733088394	2.9754572385138424E-4
28	4.760731581622148E-4	1.190182895405537E-4	0.0	0.2082224975511987	2.9754572385138424E-4
29	4.760731581622148E-4	1.7852743431083054E-4	0.0	0.4580418872968209	2.9754572385138424E-4
30	4.760731581622148E-4	1.7852743431083054E-4	0.0	0.7539808642394077	2.9754572385138424E-4
31	4.760731581622148E-4	1.7852743431083054E-4	0.0	1.6754799710071446	2.9754572385138424E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1605	0.0	29.277256	1
ATACGGC	375	0.0	26.146666	29
TAAACCG	75	1.3747922E-8	24.666666	5
GCGGTAA	1570	0.0	21.917198	23
GGTAATA	1715	0.0	20.387754	25
CGGTAAT	1685	0.0	20.311571	24
CGCGGTA	1695	0.0	20.300886	22
CTTATAC	2875	0.0	20.07652	37
GTCTAGT	65	6.904555E-5	19.923077	1
AACTCCG	1730	0.0	19.890173	5
TAATACG	1565	0.0	19.859425	27
TAACTCC	1740	0.0	19.775864	4
TAATAGT	75	9.269948E-6	19.733334	4
CTAACTC	1770	0.0	19.545198	3
GCTAACT	1790	0.0	19.223463	2
CGTGCCA	1825	0.0	19.057533	10
AATACGG	1660	0.0	18.72289	28
GTAATAC	1850	0.0	18.5	26
CGTTAGA	60	9.239235E-4	18.5	1
TAATTTA	80	1.6173946E-5	18.5	15
>>END_MODULE